目的:探讨姜黄素对APP/PS1双转基因鼠海马组织中三磷酸腺苷结合盒转运子A1(ATP binding cassette transport protein A1,ABCA1)和载脂蛋白A1(apolipoprotein A1,apoA1)的表达及血清中总胆固醇(total cholesterol,TC)和高密度脂蛋白(high...目的:探讨姜黄素对APP/PS1双转基因鼠海马组织中三磷酸腺苷结合盒转运子A1(ATP binding cassette transport protein A1,ABCA1)和载脂蛋白A1(apolipoprotein A1,apoA1)的表达及血清中总胆固醇(total cholesterol,TC)和高密度脂蛋白(high-density lipoprotein,HDL)含量的影响。方法:用APP/PS1双转基因鼠建立阿尔茨海默病(Alzheimer’s disease,AD)模型,不同浓度姜黄素饲料喂养6个月。免疫组化SP法检测转基因鼠的海马组织CA1区ABCA1和apoA1的表达变化。胆固醇酶法比色法检测血清中TC和HDL的含量。结果:经过不同浓度姜黄素饲喂转基因鼠后,其海马组织CA1区ABCA1和apoA1的表达增加(P=0.005和0.003;P=0.025和0.001),且血清中HDL的含量也随之增加,TC的含量逐渐减少,其差异均有统计学意义(P=0.041和0.010;P=0.046和0.002)。结论:ABCA1在AD的发生发展中起着重要的作用,姜黄素可能是通过增加ABCA1表达和升高apoA1和HDL含量降低胆固醇水平。展开更多
目的研究肝X受体-β(Liver X receptor-β,LXR-β)和三磷酸腺苷结合盒转运子A1(ATP binding cassette transport protein A1,ABCA1)蛋白在人脑胶质母细胞瘤(Glioblastoma,GBM)中的表达及相关性,并探讨其对GBM的意义。方法分析48例GBM患...目的研究肝X受体-β(Liver X receptor-β,LXR-β)和三磷酸腺苷结合盒转运子A1(ATP binding cassette transport protein A1,ABCA1)蛋白在人脑胶质母细胞瘤(Glioblastoma,GBM)中的表达及相关性,并探讨其对GBM的意义。方法分析48例GBM患者的资料,并取患者肿瘤组织石蜡切片,另取19份瘤旁正常脑组织石蜡切片为对照组,采用SP免疫组化法检测LXR-β和ABCA1蛋白的表达,并分析LXR-β与ABCA1表达的相关性。结果 LXR-β和ABCA1蛋白在GBM中的表达率分别为81.3%和77.1%,与对照组(15.8%和26.3%)相比,差异有统计学意义(P<0.001),且LXR-β与ABCA1的表达呈正相关(rs=0.500,P<0.05)。患者性别、年龄、胶质瘤复发、手术切除范围、肿瘤最大直径、术后放化疗情况与LXR-β、ABCA1蛋白的表达差异无统计学意义(P>0.05)。结论 LXR-β和ABCA1蛋白与GBM的形成和进展有一定关系,有可能成为临床诊断及治疗GBM的生物学指标。展开更多
MOLECULAR PHYSIOLLGY OF HEPATOCELLULAR TRANSPORT PROTEINS Basolaferal transport systems Na+-dependent bile salt uptake Uptake of bile salts into the liver was first isolated perfused rat liver[1],isolated hepatocyte...MOLECULAR PHYSIOLLGY OF HEPATOCELLULAR TRANSPORT PROTEINS Basolaferal transport systems Na+-dependent bile salt uptake Uptake of bile salts into the liver was first isolated perfused rat liver[1],isolated hepatocyte cultures and basolateral plasma membrane vesicles [2,4].展开更多
This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol(HDL-C).Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco’s modified Eagle’s medium(D...This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol(HDL-C).Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco’s modified Eagle’s medium(DMEM) containing glucose of various concentrations.The cells were divided into 3 groups in terms of different glucose concentrations in the cultures:Control group(5.6 mmol/L glucose),high glucose concentration groups(16.7 mmol/L and 30 mmol/L glucose).ATP-binding cassette transporter A1(ABCA1) mRNA expression in the macrophages was detected by semi-quantitative RT-PCR 24,48 and 72 h after glucose treatment.The results showed that ABCA1 mRNA expression in the 16.7 mmol/L glucose group was not significantly different from that in the control group at all testing time points(P>0.05 for each).In the 30 mmol/L glucose group,macrophage ABCA1 mRNA expression was not changed significantly at 24 h(P=0.14),but was substantially decreased by 40.4% at 48 h(P=0.009) and by 48.1% at 72 h(P=0.015) as compared with that in the control group.It was concluded that ABCA1 is of vital importance for HDL-C biogenesis.High glucose may hamper HDL-C biogenesis by decreasing ABCA1 expression,which contributes to low HDL-C level in diabetes.展开更多
文摘目的:探讨姜黄素对APP/PS1双转基因鼠海马组织中三磷酸腺苷结合盒转运子A1(ATP binding cassette transport protein A1,ABCA1)和载脂蛋白A1(apolipoprotein A1,apoA1)的表达及血清中总胆固醇(total cholesterol,TC)和高密度脂蛋白(high-density lipoprotein,HDL)含量的影响。方法:用APP/PS1双转基因鼠建立阿尔茨海默病(Alzheimer’s disease,AD)模型,不同浓度姜黄素饲料喂养6个月。免疫组化SP法检测转基因鼠的海马组织CA1区ABCA1和apoA1的表达变化。胆固醇酶法比色法检测血清中TC和HDL的含量。结果:经过不同浓度姜黄素饲喂转基因鼠后,其海马组织CA1区ABCA1和apoA1的表达增加(P=0.005和0.003;P=0.025和0.001),且血清中HDL的含量也随之增加,TC的含量逐渐减少,其差异均有统计学意义(P=0.041和0.010;P=0.046和0.002)。结论:ABCA1在AD的发生发展中起着重要的作用,姜黄素可能是通过增加ABCA1表达和升高apoA1和HDL含量降低胆固醇水平。
文摘目的研究肝X受体-β(Liver X receptor-β,LXR-β)和三磷酸腺苷结合盒转运子A1(ATP binding cassette transport protein A1,ABCA1)蛋白在人脑胶质母细胞瘤(Glioblastoma,GBM)中的表达及相关性,并探讨其对GBM的意义。方法分析48例GBM患者的资料,并取患者肿瘤组织石蜡切片,另取19份瘤旁正常脑组织石蜡切片为对照组,采用SP免疫组化法检测LXR-β和ABCA1蛋白的表达,并分析LXR-β与ABCA1表达的相关性。结果 LXR-β和ABCA1蛋白在GBM中的表达率分别为81.3%和77.1%,与对照组(15.8%和26.3%)相比,差异有统计学意义(P<0.001),且LXR-β与ABCA1的表达呈正相关(rs=0.500,P<0.05)。患者性别、年龄、胶质瘤复发、手术切除范围、肿瘤最大直径、术后放化疗情况与LXR-β、ABCA1蛋白的表达差异无统计学意义(P>0.05)。结论 LXR-β和ABCA1蛋白与GBM的形成和进展有一定关系,有可能成为临床诊断及治疗GBM的生物学指标。
基金supported by the Biotechnology and Biological Sciences Research Council and the Cystic Fibrosis Trust.H Li was supported by EuroCareCF(LSHM-CT-2005-018932)while J-H Chen and Z Xu were supported by scholarships from the University of Bristol and ORS awards from Universities UK.
基金supported by"H+Die Spitaler der Schweiz" the Swiss Agency for Development and Cooperation(DEZA)by the University Hospital Zurich/Switzerland
文摘MOLECULAR PHYSIOLLGY OF HEPATOCELLULAR TRANSPORT PROTEINS Basolaferal transport systems Na+-dependent bile salt uptake Uptake of bile salts into the liver was first isolated perfused rat liver[1],isolated hepatocyte cultures and basolateral plasma membrane vesicles [2,4].
基金supported by a grant from the Scientific Research Foundation for the Returned Overseas Chinese Scholars by the State Education Ministry of China (No.2005383-6144)
文摘This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol(HDL-C).Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco’s modified Eagle’s medium(DMEM) containing glucose of various concentrations.The cells were divided into 3 groups in terms of different glucose concentrations in the cultures:Control group(5.6 mmol/L glucose),high glucose concentration groups(16.7 mmol/L and 30 mmol/L glucose).ATP-binding cassette transporter A1(ABCA1) mRNA expression in the macrophages was detected by semi-quantitative RT-PCR 24,48 and 72 h after glucose treatment.The results showed that ABCA1 mRNA expression in the 16.7 mmol/L glucose group was not significantly different from that in the control group at all testing time points(P>0.05 for each).In the 30 mmol/L glucose group,macrophage ABCA1 mRNA expression was not changed significantly at 24 h(P=0.14),but was substantially decreased by 40.4% at 48 h(P=0.009) and by 48.1% at 72 h(P=0.015) as compared with that in the control group.It was concluded that ABCA1 is of vital importance for HDL-C biogenesis.High glucose may hamper HDL-C biogenesis by decreasing ABCA1 expression,which contributes to low HDL-C level in diabetes.
