Objective：To explore the effects of Iptakalim on intracellular free calcium concentration and on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1 （ET-1） in vitro. ...Objective：To explore the effects of Iptakalim on intracellular free calcium concentration and on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1 （ET-1） in vitro. Methods：A cell culture model, [^3H]-thymidine（[^3H]-TdR） incorporation test and confocal microscope were used to observe proliferation and intracellular free calcium concentration（[Ca^2±]） of rabbit PASMC induced by ET-1 in vitro. Results：The value of [^3H]-TdR incorporation in ET-1 group was increased 1.468 times higher than that in control group. Iptakalim at the concentration of 10^-7mol/L, 10^-6mol/L ,10^-5 mol/L lowered [^3H]-TdR incorporation by （19.8 ± 4.6）%, （41.2 ± 9.5）%, （54.7 ± 10.1）%, respectively, compared with the value of the cells treated with ET-1（P〈 0.01）; The intracellular fluorescence intensity of PASMC in ET-1 group was increased from 73.70 ± 10.12 to 143.84 ± 28.23, significantly higher than that in control group（P 〈 0.01）; whereas with Iptakalim,the fluorescence intensity（FI） was only increased from 74.30 ± 10.20 to 86.03 ± 9.82, significantly lower than that in ET-1 group（P 〈 0.01）. Conclusion：Iptakalim inhibited proliferation of PASMC and decreased intracellular free calcium concentration of cultured rabbit PASMC induced by ET-1.展开更多
基金This research was supported by the Innovation Fund of Nanjing Medical University(CX2003002)Natural Science Foundation of Jiangsu Province(BK2006246)+1 种基金Science Development Project of Jiangsu Science and Technology Department(BJ200051)and College Science Research Project of Jiangsu Education Department(OOKJBS20009).
文摘Objective：To explore the effects of Iptakalim on intracellular free calcium concentration and on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1 （ET-1） in vitro. Methods：A cell culture model, [^3H]-thymidine（[^3H]-TdR） incorporation test and confocal microscope were used to observe proliferation and intracellular free calcium concentration（[Ca^2±]） of rabbit PASMC induced by ET-1 in vitro. Results：The value of [^3H]-TdR incorporation in ET-1 group was increased 1.468 times higher than that in control group. Iptakalim at the concentration of 10^-7mol/L, 10^-6mol/L ,10^-5 mol/L lowered [^3H]-TdR incorporation by （19.8 ± 4.6）%, （41.2 ± 9.5）%, （54.7 ± 10.1）%, respectively, compared with the value of the cells treated with ET-1（P〈 0.01）; The intracellular fluorescence intensity of PASMC in ET-1 group was increased from 73.70 ± 10.12 to 143.84 ± 28.23, significantly higher than that in control group（P 〈 0.01）; whereas with Iptakalim,the fluorescence intensity（FI） was only increased from 74.30 ± 10.20 to 86.03 ± 9.82, significantly lower than that in ET-1 group（P 〈 0.01）. Conclusion：Iptakalim inhibited proliferation of PASMC and decreased intracellular free calcium concentration of cultured rabbit PASMC induced by ET-1.
