目的:总结后前路联合手术治疗颈椎后纵韧带骨化症(ossification of posterior longitudinal ligament,OPLL)的效果。方法:2002年9月～2006年1月采用后前路联合手术治疗25例重型颈椎后纵韧带骨化症患者,男17例,女8例;年龄32～75岁,平均5...目的:总结后前路联合手术治疗颈椎后纵韧带骨化症(ossification of posterior longitudinal ligament,OPLL)的效果。方法:2002年9月～2006年1月采用后前路联合手术治疗25例重型颈椎后纵韧带骨化症患者,男17例,女8例;年龄32～75岁,平均53.5岁。均先采用颈椎后路单开门椎管成形术,1周后再行颈椎前路减压植骨或钛网置入内固定术。前路减压涉及1个椎间隙2例,2个椎间隙1个椎体10例,3个椎间隙2个椎体及以上13例。术前、术后依据JOA评分标准评估患者的神经功能,比较术前、术后随访时JOA评分。结果:术后并发脑脊液漏2例,节断性神经根麻痹1例,并发颈部血肿者3例。随访3～24个月,平均13.5个月,术前患者JOA评分为7.1±1.1分,术后为14.9±0.9分,3个月随访时为15.1±0.8分,术后3个月平均改善率为79.48%。结论:采用先行后路椎管扩大成形术1周后再行前路减压植骨术治疗颈椎OPLL可彻底切除病灶、使脊髓减压完全,疗效较好。展开更多
Objective： To evaluate the clinical efficacy and quality of life of combined warming needle and patented Chinese medicine for patients with irritable bowel syndrome （IBS） due to liver-qi stagnation with spleen defi...Objective： To evaluate the clinical efficacy and quality of life of combined warming needle and patented Chinese medicine for patients with irritable bowel syndrome （IBS） due to liver-qi stagnation with spleen deficiency. Method： Sixty IBS cases were randomized into a treatment or control group by single-blind method. Warming needles on Tianshu （ST 25） and Dachangshu （BL 25） combined with patented Chinese medicine were adopted for cases in the treatment group, whereas the patented Chinese medicine alone was adopted in the control group. Results： The therapeutic efficacies in the two groups did not show substantial differences. The main symptoms were significantly improved after the treatment （P〈0.01）. After 2 weeks of treatment, the cases in the treatment group obtained a better improvement than the control group （P〈0.01, P〈0.05） in the severity or frequency of abdominal pain, abdominal distension, restlessness, insomnia, anxiety, suspiciousness, and loose stools with a sense of incomplete emptying. The patient＇s quality of life was improved in both groups （P〈0.01）. Conclusion： Both treatment methods could improve the clinical symptoms and increase the patient＇s quality of life. The total effective rate in the treatment group was slightly higher than the control group. Also, the combined warming needle and Chinese herbs could improve the main symptoms in a faster way.展开更多
Insect wings are subject to strong selective pressure,resulting in the evolution of remarkably diverse wing morphologies that largely determine flight capacity.However,the genetic basis and regulatory mechanisms under...Insect wings are subject to strong selective pressure,resulting in the evolution of remarkably diverse wing morphologies that largely determine flight capacity.However,the genetic basis and regulatory mechanisms underlying wing size and shape development are not well understood.The silkworm Bombyx mori micropterous(mp)mutant exhibits shortened wing length and enlarged vein spacings,albeit without changes in total wing area.Thus,the mp mutant comprises a valuable genetic resource for studying wing de-velopment.In this study,we used molecular mapping to identify the gene responsible for the mp phenotype and designated it Bmmp.Phenotype-causing mutations were identified as indels and single nucleotide polymorphisms in noncoding regions.These mutations resulted in decreased Bmmp messenger RNA levels and changes in transcript isoform composition.Bmmp null mutants were generated by clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 and exhibited changed wing shape,similar to mp mutants,and significantly smaller total wing area.By examining the expression of genes critical to wingdevelopment inwildtype and Bmmp null mutants,we found that Bmmp exerts its function by coordinately modulating anterior-posterior and proximal-distal axes development.We also studied a Drosophila mp mutant and found that Bmmp is functionally conserved in Drosophila.The Drosophila mp mutant strain exhibits curly wings of reduced size and a complete loss of flight capacity.Our results increase our understanding of the mechanisms underpinning insect wing development and reveal potential targets for pest control.展开更多
BACKGROUND Thoracolumbar fractures are generally combined with spinal cord injury to varying degrees,which may cause deterioration of the patients’condition and increase the difficulty of clinical treatment.At presen...BACKGROUND Thoracolumbar fractures are generally combined with spinal cord injury to varying degrees,which may cause deterioration of the patients’condition and increase the difficulty of clinical treatment.At present,anterior or combined anterior-posterior surgery is preferred for severe thoracolumbar fractures.AIM To investigate the effectiveness and postoperative rehabilitation of one-stage combined anterior-posterior surgery for severe thoracolumbar fractures with spinal cord injury.METHODS One-hundred-and-twenty patients who received surgery for severe thoracolumbar fractures with spinal cord injury at our hospital from February 2018 to February 2020 were randomly enrolled.They were randomly divided into group 1(one-stage combined anterior-posterior surgery,n=60)and group 2(onestage anterior-approach surgery,n=60).Treatment efficacy was compared between the two groups.RESULTS Blood loss was greater and the operation time was longer in group 1 than in group 2,and the differences were statistically significant(P<0.05).Incision length,intraoperative X-rays,and length of hospital stay were not significantly different between the two groups(P>0.05).Preoperative function of the affected vertebrae was not significantly different between the two groups(P>0.05).In each group,the patients showed significant improvement after surgery.The anterior vertebral height ratio and the posterior vertebral height ratio in group 1 after surgery were significantly higher than those in group 2.The Cobb angle after surgery was significantly lower in group 1 than in group 2(P<0.05).The canal-occupying ratio of the affected vertebrae was not significantly different between the two groups(P>0.05).Before surgery,there was no significant difference in the quality of life scores between the two groups(P>0.05).The above indicators were significantly improved after surgery compared with before surgery in each group.In addition,these indicators were markedly better in group 1 than in group 2 after surgery(P<0.05 for each).CONCLUSION One-st展开更多
BACKGROUND The coaxial radiography-guided puncture technique(CR-PT)is a novel technique for endoscopic lumbar discectomy.As the X-ray beam and the puncturing needle are maintained in a parallel and coaxial direction,t...BACKGROUND The coaxial radiography-guided puncture technique(CR-PT)is a novel technique for endoscopic lumbar discectomy.As the X-ray beam and the puncturing needle are maintained in a parallel and coaxial direction,the X-ray beam can be used to guide the trajectory angle,facilitating the choice of the puncture site and providing real-time guidance.This puncture technique offers numerous advantages over the conventional anterior-posterior and lateral radiography-guided puncture technique(AP-PT),especially in cases of herniated lumbar discs with a hypertrophied transverse process or articular process,high iliac crest,and narrowed intervertebral foramen.AIM To confirm whether CR-PT is a superior approach to percutaneous transforaminal endoscopic lumbar discectomy compared to AP-PT.METHODS In this parallel,controlled,randomized clinical trial,herniated lumbar disc patients appointed to receive percutaneous endoscopic lumbar discectomy treatment were recruited from the Pain Management Department of the Affiliated Hospital of Xuzhou Medical University and Nantong Hospital of Traditional Chinese Medicine.Sixty-five participants were enrolled and divided into either a CR-PT group or an AP-PT group.The CR-PT group underwent CR-PT,and the AP-PT group underwent AP-PT.The number of fluoroscopies during puncturing,puncture duration(min),surgery duration(min),VAS score during puncturing,and puncture success rate were recorded.RESULTS Sixty-five participants were included,with 31 participants in the CR-PT group and 34 in the AP-PT group.One participant in the AP-PT group dropped out due to unsuccessful puncturing.The number of fluoroscopies[median(P25,P75)]was 12(11,14)in the CR-PT group vs 16(12,23)in the AP-PT group,while the puncture duration(mean±SD)was 20.42±5.78 vs 25.06±5.46,respectively.The VAS score was 3(2,4)in the CR-PT group vs 3(3,4)in the AP-PT group.Further subgroup analysis was performed,considering only the participants with L5/S1 segment herniation:9 patients underwent CR-PT,and 9 underwent AP-PT.The numbe展开更多
文摘目的:总结后前路联合手术治疗颈椎后纵韧带骨化症(ossification of posterior longitudinal ligament,OPLL)的效果。方法:2002年9月～2006年1月采用后前路联合手术治疗25例重型颈椎后纵韧带骨化症患者,男17例,女8例;年龄32～75岁,平均53.5岁。均先采用颈椎后路单开门椎管成形术,1周后再行颈椎前路减压植骨或钛网置入内固定术。前路减压涉及1个椎间隙2例,2个椎间隙1个椎体10例,3个椎间隙2个椎体及以上13例。术前、术后依据JOA评分标准评估患者的神经功能,比较术前、术后随访时JOA评分。结果:术后并发脑脊液漏2例,节断性神经根麻痹1例,并发颈部血肿者3例。随访3～24个月,平均13.5个月,术前患者JOA评分为7.1±1.1分,术后为14.9±0.9分,3个月随访时为15.1±0.8分,术后3个月平均改善率为79.48%。结论:采用先行后路椎管扩大成形术1周后再行前路减压植骨术治疗颈椎OPLL可彻底切除病灶、使脊髓减压完全,疗效较好。
文摘Objective： To evaluate the clinical efficacy and quality of life of combined warming needle and patented Chinese medicine for patients with irritable bowel syndrome （IBS） due to liver-qi stagnation with spleen deficiency. Method： Sixty IBS cases were randomized into a treatment or control group by single-blind method. Warming needles on Tianshu （ST 25） and Dachangshu （BL 25） combined with patented Chinese medicine were adopted for cases in the treatment group, whereas the patented Chinese medicine alone was adopted in the control group. Results： The therapeutic efficacies in the two groups did not show substantial differences. The main symptoms were significantly improved after the treatment （P〈0.01）. After 2 weeks of treatment, the cases in the treatment group obtained a better improvement than the control group （P〈0.01, P〈0.05） in the severity or frequency of abdominal pain, abdominal distension, restlessness, insomnia, anxiety, suspiciousness, and loose stools with a sense of incomplete emptying. The patient＇s quality of life was improved in both groups （P〈0.01）. Conclusion： Both treatment methods could improve the clinical symptoms and increase the patient＇s quality of life. The total effective rate in the treatment group was slightly higher than the control group. Also, the combined warming needle and Chinese herbs could improve the main symptoms in a faster way.
基金supported by the National Natural Science Foundation of China(awards U20A2058 and 31830094).
文摘Insect wings are subject to strong selective pressure,resulting in the evolution of remarkably diverse wing morphologies that largely determine flight capacity.However,the genetic basis and regulatory mechanisms underlying wing size and shape development are not well understood.The silkworm Bombyx mori micropterous(mp)mutant exhibits shortened wing length and enlarged vein spacings,albeit without changes in total wing area.Thus,the mp mutant comprises a valuable genetic resource for studying wing de-velopment.In this study,we used molecular mapping to identify the gene responsible for the mp phenotype and designated it Bmmp.Phenotype-causing mutations were identified as indels and single nucleotide polymorphisms in noncoding regions.These mutations resulted in decreased Bmmp messenger RNA levels and changes in transcript isoform composition.Bmmp null mutants were generated by clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 and exhibited changed wing shape,similar to mp mutants,and significantly smaller total wing area.By examining the expression of genes critical to wingdevelopment inwildtype and Bmmp null mutants,we found that Bmmp exerts its function by coordinately modulating anterior-posterior and proximal-distal axes development.We also studied a Drosophila mp mutant and found that Bmmp is functionally conserved in Drosophila.The Drosophila mp mutant strain exhibits curly wings of reduced size and a complete loss of flight capacity.Our results increase our understanding of the mechanisms underpinning insect wing development and reveal potential targets for pest control.
文摘BACKGROUND Thoracolumbar fractures are generally combined with spinal cord injury to varying degrees,which may cause deterioration of the patients’condition and increase the difficulty of clinical treatment.At present,anterior or combined anterior-posterior surgery is preferred for severe thoracolumbar fractures.AIM To investigate the effectiveness and postoperative rehabilitation of one-stage combined anterior-posterior surgery for severe thoracolumbar fractures with spinal cord injury.METHODS One-hundred-and-twenty patients who received surgery for severe thoracolumbar fractures with spinal cord injury at our hospital from February 2018 to February 2020 were randomly enrolled.They were randomly divided into group 1(one-stage combined anterior-posterior surgery,n=60)and group 2(onestage anterior-approach surgery,n=60).Treatment efficacy was compared between the two groups.RESULTS Blood loss was greater and the operation time was longer in group 1 than in group 2,and the differences were statistically significant(P<0.05).Incision length,intraoperative X-rays,and length of hospital stay were not significantly different between the two groups(P>0.05).Preoperative function of the affected vertebrae was not significantly different between the two groups(P>0.05).In each group,the patients showed significant improvement after surgery.The anterior vertebral height ratio and the posterior vertebral height ratio in group 1 after surgery were significantly higher than those in group 2.The Cobb angle after surgery was significantly lower in group 1 than in group 2(P<0.05).The canal-occupying ratio of the affected vertebrae was not significantly different between the two groups(P>0.05).Before surgery,there was no significant difference in the quality of life scores between the two groups(P>0.05).The above indicators were significantly improved after surgery compared with before surgery in each group.In addition,these indicators were markedly better in group 1 than in group 2 after surgery(P<0.05 for each).CONCLUSION One-st
文摘BACKGROUND The coaxial radiography-guided puncture technique(CR-PT)is a novel technique for endoscopic lumbar discectomy.As the X-ray beam and the puncturing needle are maintained in a parallel and coaxial direction,the X-ray beam can be used to guide the trajectory angle,facilitating the choice of the puncture site and providing real-time guidance.This puncture technique offers numerous advantages over the conventional anterior-posterior and lateral radiography-guided puncture technique(AP-PT),especially in cases of herniated lumbar discs with a hypertrophied transverse process or articular process,high iliac crest,and narrowed intervertebral foramen.AIM To confirm whether CR-PT is a superior approach to percutaneous transforaminal endoscopic lumbar discectomy compared to AP-PT.METHODS In this parallel,controlled,randomized clinical trial,herniated lumbar disc patients appointed to receive percutaneous endoscopic lumbar discectomy treatment were recruited from the Pain Management Department of the Affiliated Hospital of Xuzhou Medical University and Nantong Hospital of Traditional Chinese Medicine.Sixty-five participants were enrolled and divided into either a CR-PT group or an AP-PT group.The CR-PT group underwent CR-PT,and the AP-PT group underwent AP-PT.The number of fluoroscopies during puncturing,puncture duration(min),surgery duration(min),VAS score during puncturing,and puncture success rate were recorded.RESULTS Sixty-five participants were included,with 31 participants in the CR-PT group and 34 in the AP-PT group.One participant in the AP-PT group dropped out due to unsuccessful puncturing.The number of fluoroscopies[median(P25,P75)]was 12(11,14)in the CR-PT group vs 16(12,23)in the AP-PT group,while the puncture duration(mean±SD)was 20.42±5.78 vs 25.06±5.46,respectively.The VAS score was 3(2,4)in the CR-PT group vs 3(3,4)in the AP-PT group.Further subgroup analysis was performed,considering only the participants with L5/S1 segment herniation:9 patients underwent CR-PT,and 9 underwent AP-PT.The numbe
