AIM To inquire into the ERCP and CT features of pancreas divisum (PD) and its role in the etiology of chronic pancreatitis. METHODS Fourteen patients with PD were analyzed in regard to the findings in ERCP and CT, ...AIM To inquire into the ERCP and CT features of pancreas divisum (PD) and its role in the etiology of chronic pancreatitis. METHODS Fourteen patients with PD were analyzed in regard to the findings in ERCP and CT, the activities of serum amylase and the incidence of pancreatitis. Dorsal ductography via minor papilla cannulation was performed in six of them. RESULTS The length of dorsal and ventral pancreatic duct was 16 56cm±2 52cm and 5 55cm±1 46cm. Most of the patients had dilatation of dorsal (10/14) and ventral (8/14) duct and the stenosis of dorsal duct terminal (10/14). Delayed clearance of contrast in dorsal duct was found in 8 patients. The size and contour of the pancreas were normal in all the patients at conventional CT. Pancreatitis was identified in 13 patients. CONCLUSION Dorsal ductography was necessary in the diagnosis of PD. Conventional CT play little role in the diagnosis of PD. Patients with PD run a higher risk of pancreatitis due to the stenosis of the minor papilla.展开更多
AIM To investigate the potential role of intestinal microflora barrier in the pathogenesis of pancreatic infection. METHODS Fifteen dogs were colonized with a strain of E.coli JM109 bearing ampicillin resistan...AIM To investigate the potential role of intestinal microflora barrier in the pathogenesis of pancreatic infection. METHODS Fifteen dogs were colonized with a strain of E.coli JM109 bearing ampicillin resistance plasmid PUC18. The animals were divided into two groups. In experimental group ( n =8), acute necrotizing pancreatitis (ANP) was induced by injection of 0 5ml/kg of sodium tarocholate with 3000U/kg trypsin into the pancreatic duct. The control group ( n =7) underwent laparotomy only. All animals were sacrificed 7 days later. Mucosal and luminal microflora of intestine were analyzed quantitatively, and various organs were harvested for culturing, blood samples were obtained for determination of serum amylase activities and plasma lipopolysaccharide (LPS) concentrations. RESULTS In the experimental group, the number of E.coli in the intestine was much higher than those of the controls, while bifidobacterium and lactobacillus were decreased significantly (jejunum, 1 75±0 95 vs 2 35±0 79, P <0 05; 1 13±0 8 vs 1 83±0 64, P <0 05; ileum, 2 89±0 86 vs 3 87±1 05, P <0 05; 1 78±0 79 vs 3 79±1 11, P <0 01; cecum, 2 70±0 88 vs 4 89±0 87, P <001; 2 81±0 73 vs 3 24±0 84, P <0 05. Content of cecum, 3 06±0 87 vs 5 15±1 44, P <0 01; 2 67±0 61 vs 4 25±0 81, P <0 01), resulting in reversal of bifido bacterium/ E.coli ratio as compared with the control group (jejunum, 0 51±0 76 vs 1 23±0 53, P <0 05; ileum, 0 62±0 68 vs 1 16±0 32, P <0 05; cecum, 0 46±0 44 vs 1 03±0 64, P <0 05). In addition, intestinal bacteria were isolated from organs of all animals in the experimental group, and JM109 was also detected in most cases. Positive blood culture was 75 0% and 62 5% on day 1 and 2 after induction of ANP, respectively, but no bacterium was found in the controls. As compared with the control group, blood LPS levels and serum amylase activities in展开更多
AIM To observe the protective effect of combined i.v. administration of Yuanhu injection (YHI) and Huoxuehuayu injection I (HHI I) against acute pancreatitis (AP) in rabbits. METHODS Severe acute pancreatitis...AIM To observe the protective effect of combined i.v. administration of Yuanhu injection (YHI) and Huoxuehuayu injection I (HHI I) against acute pancreatitis (AP) in rabbits. METHODS Severe acute pancreatitis (SAP) was induced by retrograde infusion of artificial bile juice into biliary pancreatic duct, and treated with YHI and HHI I intravenously. The protective effect was judged by the survival time and rate, serum amylase, serum interleukin 6, pancreatic microcirculation and pathological alteration. RESULTS Combined use of YHI and HHI I could markedly increase the rabbits′ 5 day survival rate after AP (83 3% in the treatment group and 33 3% in control). The serum amylase value ( ±s ) decreased to 1596 6U/L ± 760 50U/L in the 5th day from the high level ( 6320 83U/L ± 2614 12U/L ) in the 1st day after AP in the treatment group, while in the control group the amylase activity in the 5th day was 2095 0U/L ± 1081 87U/L , being significantly different from that before AP ( 837 17U/L ± 189 12U/L ). YHI and HHI I also obviously improved the pancreatic microcirculation and lowered the serum interleukin 6 level, one of the indices of severe pancreatitis. Pathological examination indicated all the changes typical for AP in YHI and HHI I treatment group were milder than those in the control. CONCLUSION YHI and HHI I used in combination might have protective effect against acute pancreatitis in rabbits.展开更多
文摘AIM To inquire into the ERCP and CT features of pancreas divisum (PD) and its role in the etiology of chronic pancreatitis. METHODS Fourteen patients with PD were analyzed in regard to the findings in ERCP and CT, the activities of serum amylase and the incidence of pancreatitis. Dorsal ductography via minor papilla cannulation was performed in six of them. RESULTS The length of dorsal and ventral pancreatic duct was 16 56cm±2 52cm and 5 55cm±1 46cm. Most of the patients had dilatation of dorsal (10/14) and ventral (8/14) duct and the stenosis of dorsal duct terminal (10/14). Delayed clearance of contrast in dorsal duct was found in 8 patients. The size and contour of the pancreas were normal in all the patients at conventional CT. Pancreatitis was identified in 13 patients. CONCLUSION Dorsal ductography was necessary in the diagnosis of PD. Conventional CT play little role in the diagnosis of PD. Patients with PD run a higher risk of pancreatitis due to the stenosis of the minor papilla.
文摘AIM To investigate the potential role of intestinal microflora barrier in the pathogenesis of pancreatic infection. METHODS Fifteen dogs were colonized with a strain of E.coli JM109 bearing ampicillin resistance plasmid PUC18. The animals were divided into two groups. In experimental group ( n =8), acute necrotizing pancreatitis (ANP) was induced by injection of 0 5ml/kg of sodium tarocholate with 3000U/kg trypsin into the pancreatic duct. The control group ( n =7) underwent laparotomy only. All animals were sacrificed 7 days later. Mucosal and luminal microflora of intestine were analyzed quantitatively, and various organs were harvested for culturing, blood samples were obtained for determination of serum amylase activities and plasma lipopolysaccharide (LPS) concentrations. RESULTS In the experimental group, the number of E.coli in the intestine was much higher than those of the controls, while bifidobacterium and lactobacillus were decreased significantly (jejunum, 1 75±0 95 vs 2 35±0 79, P <0 05; 1 13±0 8 vs 1 83±0 64, P <0 05; ileum, 2 89±0 86 vs 3 87±1 05, P <0 05; 1 78±0 79 vs 3 79±1 11, P <0 01; cecum, 2 70±0 88 vs 4 89±0 87, P <001; 2 81±0 73 vs 3 24±0 84, P <0 05. Content of cecum, 3 06±0 87 vs 5 15±1 44, P <0 01; 2 67±0 61 vs 4 25±0 81, P <0 01), resulting in reversal of bifido bacterium/ E.coli ratio as compared with the control group (jejunum, 0 51±0 76 vs 1 23±0 53, P <0 05; ileum, 0 62±0 68 vs 1 16±0 32, P <0 05; cecum, 0 46±0 44 vs 1 03±0 64, P <0 05). In addition, intestinal bacteria were isolated from organs of all animals in the experimental group, and JM109 was also detected in most cases. Positive blood culture was 75 0% and 62 5% on day 1 and 2 after induction of ANP, respectively, but no bacterium was found in the controls. As compared with the control group, blood LPS levels and serum amylase activities in
文摘AIM To observe the protective effect of combined i.v. administration of Yuanhu injection (YHI) and Huoxuehuayu injection I (HHI I) against acute pancreatitis (AP) in rabbits. METHODS Severe acute pancreatitis (SAP) was induced by retrograde infusion of artificial bile juice into biliary pancreatic duct, and treated with YHI and HHI I intravenously. The protective effect was judged by the survival time and rate, serum amylase, serum interleukin 6, pancreatic microcirculation and pathological alteration. RESULTS Combined use of YHI and HHI I could markedly increase the rabbits′ 5 day survival rate after AP (83 3% in the treatment group and 33 3% in control). The serum amylase value ( ±s ) decreased to 1596 6U/L ± 760 50U/L in the 5th day from the high level ( 6320 83U/L ± 2614 12U/L ) in the 1st day after AP in the treatment group, while in the control group the amylase activity in the 5th day was 2095 0U/L ± 1081 87U/L , being significantly different from that before AP ( 837 17U/L ± 189 12U/L ). YHI and HHI I also obviously improved the pancreatic microcirculation and lowered the serum interleukin 6 level, one of the indices of severe pancreatitis. Pathological examination indicated all the changes typical for AP in YHI and HHI I treatment group were milder than those in the control. CONCLUSION YHI and HHI I used in combination might have protective effect against acute pancreatitis in rabbits.
