Objective:To examine the effect of moxibustion on the wound healing process and its mechanism using a rat wound model.Methods:Sixty male Sprague-Dawley rats were randomly divided into a sham-treated group(n=30,wound s...Objective:To examine the effect of moxibustion on the wound healing process and its mechanism using a rat wound model.Methods:Sixty male Sprague-Dawley rats were randomly divided into a sham-treated group(n=30,wound surgery only)and a moxibustion group(n=30,wound treated with moxibustion).Circular fullthick ness skin wounds were produced in rats.Moxibustion was applied to the edge of wound and was continued on alternating days till 14 days after surgery,followed by measurement of wound size.Expression of collagens,prolyl-4-hydroxylase(P4H)and transtorming growth factor-β(TGF-β)were evaluated by histochemical study and real-time polymerase chain reaction.Results:The size of the wound lesion was significantly reduced in rats treated with moxibustion as compared to that in sham-treated rats at 4-10 days after wounding(P<0.01).Moxibusti on stimulated mRNA expressi on of collage ns at 4 days(P<0.01),but not at 7 days,accompa nied by enhanced proliferation of P4H-positive fibroblasts.Of importanee,expression of TGF-β in tissue from the wound lesio n treated with moxibusti on was sign ifica ntly in creased as compared to that in sham-treated rats at 4 days(P<0.01 or P<0.05),but not at 7 days.Conclusions:The treatment with moxibustion promoted the wound healing process in the early phase through proliferation of fibroblasts and rapid formation of granulation,possibly mediated by induction of TGF-β which is a key molecule in the physiological process of wound healing.Moxibustion can be expected to be effective as complementary treatment for intractable ulcers.展开更多
AIM:To evaluate the serum levels of endozepine-4,their relation with ammonia serum levels,the grading of coma and the severity of cirrhosis,in patients with hepatic coma. METHODS:In this study we included 20 subjects ...AIM:To evaluate the serum levels of endozepine-4,their relation with ammonia serum levels,the grading of coma and the severity of cirrhosis,in patients with hepatic coma. METHODS:In this study we included 20 subjects with Hepatic coma,20 subjects with minimal hepatic encephalopathy(MHE) and 20 subjects control. All subjects underwent blood analysis,Child Pugh and Model for End- stage liver disease(MELD) assessment,endozepine-4 analysis. RESULTS:Subjects with hepatic coma showed significant difference in endozepine-4(P < 0.001) and NH3 levels(P < 0.001) compared both to MHE and controls patients. Between NH3 and endozepine-4 we observed a significant correlation(P = 0.009; Pearson correlation 0.570). There was a significant correlation between endozepine-4 and MELD(P = 0.017; Pearsoncorrelation = 0.529). In our study blood ammonia concentration was noted to be raised in patients with hepatic coma,with the highest ammonia levels being found in those who were comatose. We also found a high correlation between endozepine-4 and ammonia(P < 0.001). In patients with grade Ⅳ hepatic coma,endozepine levels were significantly higher compared to other groups. CONCLUSION:This study suggests that an increased level of endozepine in subjects with higher levels of MELD was observed. In conclusion,data concerning involvement of the GABA-ergic system in HE coma could be explained by stage-specific alterations.展开更多
在拉丁语中,用来表示英语“Other”一词的是“alter”。就alter本身而言,它在英语里具有become different or making something other”的涵义。汉语可译为“改变”。例如: Iwant to alter the original plan. (我想改变原计划。) 要改...在拉丁语中,用来表示英语“Other”一词的是“alter”。就alter本身而言,它在英语里具有become different or making something other”的涵义。汉语可译为“改变”。例如: Iwant to alter the original plan. (我想改变原计划。) 要改变原计划,就得提出其它的想法(other ideas),其它的意见(other opinions)和其它的建议(other suggestions)等。换句话说就得对原计划作出较大的“更改”(alteration)。 “Alteration”一词是“alter”的名词形式。展开更多
文摘Objective:To examine the effect of moxibustion on the wound healing process and its mechanism using a rat wound model.Methods:Sixty male Sprague-Dawley rats were randomly divided into a sham-treated group(n=30,wound surgery only)and a moxibustion group(n=30,wound treated with moxibustion).Circular fullthick ness skin wounds were produced in rats.Moxibustion was applied to the edge of wound and was continued on alternating days till 14 days after surgery,followed by measurement of wound size.Expression of collagens,prolyl-4-hydroxylase(P4H)and transtorming growth factor-β(TGF-β)were evaluated by histochemical study and real-time polymerase chain reaction.Results:The size of the wound lesion was significantly reduced in rats treated with moxibustion as compared to that in sham-treated rats at 4-10 days after wounding(P<0.01).Moxibusti on stimulated mRNA expressi on of collage ns at 4 days(P<0.01),but not at 7 days,accompa nied by enhanced proliferation of P4H-positive fibroblasts.Of importanee,expression of TGF-β in tissue from the wound lesio n treated with moxibusti on was sign ifica ntly in creased as compared to that in sham-treated rats at 4 days(P<0.01 or P<0.05),but not at 7 days.Conclusions:The treatment with moxibustion promoted the wound healing process in the early phase through proliferation of fibroblasts and rapid formation of granulation,possibly mediated by induction of TGF-β which is a key molecule in the physiological process of wound healing.Moxibustion can be expected to be effective as complementary treatment for intractable ulcers.
文摘AIM:To evaluate the serum levels of endozepine-4,their relation with ammonia serum levels,the grading of coma and the severity of cirrhosis,in patients with hepatic coma. METHODS:In this study we included 20 subjects with Hepatic coma,20 subjects with minimal hepatic encephalopathy(MHE) and 20 subjects control. All subjects underwent blood analysis,Child Pugh and Model for End- stage liver disease(MELD) assessment,endozepine-4 analysis. RESULTS:Subjects with hepatic coma showed significant difference in endozepine-4(P < 0.001) and NH3 levels(P < 0.001) compared both to MHE and controls patients. Between NH3 and endozepine-4 we observed a significant correlation(P = 0.009; Pearson correlation 0.570). There was a significant correlation between endozepine-4 and MELD(P = 0.017; Pearsoncorrelation = 0.529). In our study blood ammonia concentration was noted to be raised in patients with hepatic coma,with the highest ammonia levels being found in those who were comatose. We also found a high correlation between endozepine-4 and ammonia(P < 0.001). In patients with grade Ⅳ hepatic coma,endozepine levels were significantly higher compared to other groups. CONCLUSION:This study suggests that an increased level of endozepine in subjects with higher levels of MELD was observed. In conclusion,data concerning involvement of the GABA-ergic system in HE coma could be explained by stage-specific alterations.
文摘在拉丁语中,用来表示英语“Other”一词的是“alter”。就alter本身而言,它在英语里具有become different or making something other”的涵义。汉语可译为“改变”。例如: Iwant to alter the original plan. (我想改变原计划。) 要改变原计划,就得提出其它的想法(other ideas),其它的意见(other opinions)和其它的建议(other suggestions)等。换句话说就得对原计划作出较大的“更改”(alteration)。 “Alteration”一词是“alter”的名词形式。
