Background:Rubiadin is a type of anthraquinone compound that can be found in Rubiaceae plants,such as Ronas.Nonetheless,only limited research has been done to explore the potential anticancer properties of rubiadin on...Background:Rubiadin is a type of anthraquinone compound that can be found in Rubiaceae plants,such as Ronas.Nonetheless,only limited research has been done to explore the potential anticancer properties of rubiadin on liver cancer cells.Thus,the objective of the present study is to examine how rubiadin affects the viability of liver cancer cells as well as normal cells.Methods:HepG2 and AGO cell lines were assigned into controls(not exposed to rubiadin)and groups with exposure to rubiadin with 12.5,6.25,3.125,1.56,0.78,and 0.39μg/mL concentrations.3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide and reverse transcription-polymerase chain reaction were used to measure cell viability,and one-way analysis of variance was used for data analysis.Results:The viability of liver cancer cells was significantly reduced when exposed to 12.5,6.25,3.125,and 1.56μg/mL concentrations(P<0.01).An IC50 of 44.73μg/mL was reported.Furthermore,the BAX gene’s relative expression(P<0.05)was significantly increased and the BCL2 gene expression(P<0.05)was significantly reduced.The average ratio of BAX gene expression to BCL2 increased significantly(P<0.01).Conclusion:This research showed that rubiadin decreases cell viability by increasing the ratio of BAX gene expression to BCL2.In addition rubiadin has no cytotoxic effect on normal cells.展开更多
MicroRNAs （miRNAs） post-transcriptionally regulate gene expression by binding to target mRNAs with perfect or imperfect complementarity, recruiting an Argonaute （AGO） protein complex that usually results in degrad...MicroRNAs （miRNAs） post-transcriptionally regulate gene expression by binding to target mRNAs with perfect or imperfect complementarity, recruiting an Argonaute （AGO） protein complex that usually results in degradation or translational repression of the target mRNA. AGO proteins function as the Slicer enzyme in miRNA and small interfering RNA （siRNA） pathways involved in human physiological and pathophysiological processes, such as antiviral responses and disease formation. Although the past decade has witnessed rapid advancement in studies of AGO protein functions, to further elucidate the molecular mechanism of AGO proteins in cellular function and biochemical process is really a challenging area for researchers. In order to understand the molecular causes underlying the pathological processes, we mainly focus on five fundamental problems of AGO proteins, including evolution, functional domain, subcellular location, post-translational modification and protein-protein interactions. Our discussion highlight their roles in early diagnosis, disease prevention, drug target identification, drug response, etc.展开更多
在浓氢氧化钠溶液中,通过电化学方法氧化银纳米颗粒制备得到AgO有序阵列结构电极材料。性能表征表明,所制备的AgO材料具有独特的直通孔阵列结构,有利于电解质溶液在孔隙中的扩散,可直接用作Al/AgO电池阴极,无需黏结剂等。与常规AgO阴极...在浓氢氧化钠溶液中,通过电化学方法氧化银纳米颗粒制备得到AgO有序阵列结构电极材料。性能表征表明,所制备的AgO材料具有独特的直通孔阵列结构,有利于电解质溶液在孔隙中的扩散,可直接用作Al/AgO电池阴极,无需黏结剂等。与常规AgO阴极材料相比,同等条件下,以AgO有序阵列结构材料为阴极所组成电池的放电性能大幅提高,3 C倍率下质量比容量可达422.6 m A·h·g-1,电极活性材料的利用率为97.8%,7 C倍率下质量比容量依然有387.8 m A·h·g-1,活性物质利用率89.7%。同时,循环性能相比传统电极也得到大幅提升,在第10个循环时依然保持着405.2 m A·h·g-1的质量比容量。制备方法易于操作且高效环保,有利于工业化生产;所得材料具有独特结构和性能优势。展开更多
Recently, fault or health condition prediction of complex systems becomes an interesting research topic. However, it is difficult to establish precise physical model for complex systems, and the time series properties...Recently, fault or health condition prediction of complex systems becomes an interesting research topic. However, it is difficult to establish precise physical model for complex systems, and the time series properties are often necessary to be incorporated for the prediction in practice. Currently, the LS-SVR is widely adopted for prediction of systems with time series data. In this paper, in order to improve the prediction accuracy, accumulated generating operation （AGO） is carried out to improve the data quality and regularity of raw time series data based on grey system theory; then, the inverse accumulated generating operation （IAGO） is performed to obtain the prediction results. In addition, due to the reason that appropriate kernel function plays an important role in improving the accuracy of prediction through LS-SVR, a modified Gaussian radial basis function （RBF） is proposed. The requirements of distance functions-based kernel functions are satisfied, which ensure fast damping at the place adjacent to the test point and a moderate damping at infinity. The presented model is applied to the analysis of benchmarks. As indicated by the results, the proposed method is an effective prediction one with good precision.展开更多
采用正交实验法研究了液相沉淀法制备AgO材料的各影响因素,分析了影响AgO材料的规律和机理。利用化学滴定法测试其纯度,且通过扫描电子显微镜法(SEM)、X射线衍射光谱法(XRD)分析了不同纯度AgO的形貌特征。研究结果表明:制备AgO粉体的最...采用正交实验法研究了液相沉淀法制备AgO材料的各影响因素,分析了影响AgO材料的规律和机理。利用化学滴定法测试其纯度,且通过扫描电子显微镜法(SEM)、X射线衍射光谱法(XRD)分析了不同纯度AgO的形貌特征。研究结果表明:制备AgO粉体的最优工艺为:温度控制在90℃;滴定AgNO3的速率为10 m L/min;n(K2S2O8)∶n(Ag NO3)=1.2∶2;n(KOH)∶n(AgNO3)=3.0∶1;反应时间60 min;纯度高达95.7%。以KOH水溶液为电解质装配电池,测试其电化学性能,在8 C下恒流放电,终止电压为1.0V,最佳工艺制备出的样品放电比容量为382 m Ah/g,为理论值的88%。展开更多
文摘Background:Rubiadin is a type of anthraquinone compound that can be found in Rubiaceae plants,such as Ronas.Nonetheless,only limited research has been done to explore the potential anticancer properties of rubiadin on liver cancer cells.Thus,the objective of the present study is to examine how rubiadin affects the viability of liver cancer cells as well as normal cells.Methods:HepG2 and AGO cell lines were assigned into controls(not exposed to rubiadin)and groups with exposure to rubiadin with 12.5,6.25,3.125,1.56,0.78,and 0.39μg/mL concentrations.3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide and reverse transcription-polymerase chain reaction were used to measure cell viability,and one-way analysis of variance was used for data analysis.Results:The viability of liver cancer cells was significantly reduced when exposed to 12.5,6.25,3.125,and 1.56μg/mL concentrations(P<0.01).An IC50 of 44.73μg/mL was reported.Furthermore,the BAX gene’s relative expression(P<0.05)was significantly increased and the BCL2 gene expression(P<0.05)was significantly reduced.The average ratio of BAX gene expression to BCL2 increased significantly(P<0.01).Conclusion:This research showed that rubiadin decreases cell viability by increasing the ratio of BAX gene expression to BCL2.In addition rubiadin has no cytotoxic effect on normal cells.
文摘MicroRNAs （miRNAs） post-transcriptionally regulate gene expression by binding to target mRNAs with perfect or imperfect complementarity, recruiting an Argonaute （AGO） protein complex that usually results in degradation or translational repression of the target mRNA. AGO proteins function as the Slicer enzyme in miRNA and small interfering RNA （siRNA） pathways involved in human physiological and pathophysiological processes, such as antiviral responses and disease formation. Although the past decade has witnessed rapid advancement in studies of AGO protein functions, to further elucidate the molecular mechanism of AGO proteins in cellular function and biochemical process is really a challenging area for researchers. In order to understand the molecular causes underlying the pathological processes, we mainly focus on five fundamental problems of AGO proteins, including evolution, functional domain, subcellular location, post-translational modification and protein-protein interactions. Our discussion highlight their roles in early diagnosis, disease prevention, drug target identification, drug response, etc.
文摘在浓氢氧化钠溶液中,通过电化学方法氧化银纳米颗粒制备得到AgO有序阵列结构电极材料。性能表征表明,所制备的AgO材料具有独特的直通孔阵列结构,有利于电解质溶液在孔隙中的扩散,可直接用作Al/AgO电池阴极,无需黏结剂等。与常规AgO阴极材料相比,同等条件下,以AgO有序阵列结构材料为阴极所组成电池的放电性能大幅提高,3 C倍率下质量比容量可达422.6 m A·h·g-1,电极活性材料的利用率为97.8%,7 C倍率下质量比容量依然有387.8 m A·h·g-1,活性物质利用率89.7%。同时,循环性能相比传统电极也得到大幅提升,在第10个循环时依然保持着405.2 m A·h·g-1的质量比容量。制备方法易于操作且高效环保,有利于工业化生产;所得材料具有独特结构和性能优势。
基金supported by National Natural Science Foundation(NNSF)of China under Grant No.61371024Aviation Science Fund of China under Grant No.2013ZD53051+1 种基金Aerospace Technology Support Fund of Chinathe Industry-Academy-Research Project of AVIC(cxy2013XGD14)
文摘Recently, fault or health condition prediction of complex systems becomes an interesting research topic. However, it is difficult to establish precise physical model for complex systems, and the time series properties are often necessary to be incorporated for the prediction in practice. Currently, the LS-SVR is widely adopted for prediction of systems with time series data. In this paper, in order to improve the prediction accuracy, accumulated generating operation （AGO） is carried out to improve the data quality and regularity of raw time series data based on grey system theory; then, the inverse accumulated generating operation （IAGO） is performed to obtain the prediction results. In addition, due to the reason that appropriate kernel function plays an important role in improving the accuracy of prediction through LS-SVR, a modified Gaussian radial basis function （RBF） is proposed. The requirements of distance functions-based kernel functions are satisfied, which ensure fast damping at the place adjacent to the test point and a moderate damping at infinity. The presented model is applied to the analysis of benchmarks. As indicated by the results, the proposed method is an effective prediction one with good precision.
文摘采用正交实验法研究了液相沉淀法制备AgO材料的各影响因素,分析了影响AgO材料的规律和机理。利用化学滴定法测试其纯度,且通过扫描电子显微镜法(SEM)、X射线衍射光谱法(XRD)分析了不同纯度AgO的形貌特征。研究结果表明:制备AgO粉体的最优工艺为:温度控制在90℃;滴定AgNO3的速率为10 m L/min;n(K2S2O8)∶n(Ag NO3)=1.2∶2;n(KOH)∶n(AgNO3)=3.0∶1;反应时间60 min;纯度高达95.7%。以KOH水溶液为电解质装配电池,测试其电化学性能,在8 C下恒流放电,终止电压为1.0V,最佳工艺制备出的样品放电比容量为382 m Ah/g,为理论值的88%。
