应用缺氧/好氧—移动床生物膜反应器(Anoxic/Aerobic-Moving Bed Biofilm Reactor,A/O-MBBR)系统,通过固定进水COD与无机氮之比C/N为12,将COD依次设置为150、300、350和450mg·L^(-1)时,探讨反应器对海水养殖废水中氨氮、硝氮、亚...应用缺氧/好氧—移动床生物膜反应器(Anoxic/Aerobic-Moving Bed Biofilm Reactor,A/O-MBBR)系统,通过固定进水COD与无机氮之比C/N为12,将COD依次设置为150、300、350和450mg·L^(-1)时,探讨反应器对海水养殖废水中氨氮、硝氮、亚硝氮及COD的去除效果,并分析微生物群落变化及响应。结果表明,在进水COD为150mg·L^(-1)、无机氮12.5mg·L^(-1)时,反应器运行效果最佳,此时氨氮、硝氮、COD和亚硝氮的去除率分别为93.7%(出水0.3 mg·L^(-1)),87.5%(出水0.7mg·L^(-1)),98.2%(出水3mg·L^(-1)),86.9%(出水0.1mg·L^(-1))。当COD提高至450mg·L^(-1)时,氨氮去除率逐渐降低到40.7%,亚硝氮在COD为350mg·L^(-1)时去除率降低至22.5%。在整个系统运行过程中,变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)是反应器中的绝对优势类群。当COD由150mg·L^(-1)上升到350mg·L^(-1)时,变形菌门的相对丰度却由63.9%~75.2%提高到88.1%~92.4%;拟杆菌门的相对丰度则由11.9%~13.0%降低到4.5%~5.4%;引起污泥膨胀的发硫菌属(Thiothrix)的相对丰度由6.24%~7.08%增加到58.16%~76.74%,表明反应器污泥膨胀趋势加剧。应用A/O-MBBR工艺处理海水养殖废水时,在COD为150mg·L^(-1)时效果较好,随着COD浓度提高,引起污泥膨胀的微生物开始大量滋生。展开更多
In order to explore the effect of carbon and nitrogen(C/N)ratio on the performance of anoxic/aerobic-moving bed bio-film reactor(A/O-MBBR)process for treating mariculture wastewater,a laboratory-scale A/O-MBBR was con...In order to explore the effect of carbon and nitrogen(C/N)ratio on the performance of anoxic/aerobic-moving bed bio-film reactor(A/O-MBBR)process for treating mariculture wastewater,a laboratory-scale A/O-MBBR was conducted.The results showed that the reduction of C/N ratio was conducive to improving the removal efficiency of chemical oxygen demand(COD)and ammonia nitrogen(NH_(4)^(+)-N),while inhibiting that of nitrite nitrogen(NO_(2)^(−)-N)and nitrate nitrogen(NO_(3)^(−)-N).The extracellular polymeric substances(EPS)in anoxic zone were significantly higher in concentration than that in aerobic zone although they both declined with decrease of C/N ratio.The result provides solid support for better controlling the pollution of mariculture wastewater.展开更多
The wide application of antibiotics in aquaculture requires an efficient treatment of the wastewater before discharging it into the environment.During the wastewater treatment,the influence of antibiotics on the perfo...The wide application of antibiotics in aquaculture requires an efficient treatment of the wastewater before discharging it into the environment.During the wastewater treatment,the influence of antibiotics on the performance of bioreactor should be well revealed due to their toxicity to the functional microbial community.In this study,the effect of feeding 10-30 mg L−1 sulfamethoxazole(SMX)in influent on the performance of an anoxic/oxic-moving bed biofilm reactor(A/O-MBBR)treating mariculture wastewater and the responding change of biofilm microbial communities was investigated.The COD average removal rate remained at 94.61%-97.34%with the dosage of SMX.Compared with that,the nitrifying removals of NH4+-N and NO2−-N were violently inhibited by 30 mg L−1 SMX and denitrifying removal of the NO3−-N decreased obviously with 20 mg L−1 or more SMX.The microbial community in the successful startup bioreactor was relatively abundant,while the diversity of microbial community decreased with the increase of feeding SMX.The salt-tolerant and SMX-resistant genera Arcobacter,Thiothrix,Desulfuromusa and Nitrosomonas were gradually enriched and finally played a vital role in converting COD and recycling nitrogen and sulfur.Hence,the present A/O-MBBR reactor with the salt-tolerant functional microbiota achieved efficient removal of pollutants in the presence of low concentration(e.g.,10 mg L−1)SMX.展开更多
文摘应用缺氧/好氧—移动床生物膜反应器(Anoxic/Aerobic-Moving Bed Biofilm Reactor,A/O-MBBR)系统,通过固定进水COD与无机氮之比C/N为12,将COD依次设置为150、300、350和450mg·L^(-1)时,探讨反应器对海水养殖废水中氨氮、硝氮、亚硝氮及COD的去除效果,并分析微生物群落变化及响应。结果表明,在进水COD为150mg·L^(-1)、无机氮12.5mg·L^(-1)时,反应器运行效果最佳,此时氨氮、硝氮、COD和亚硝氮的去除率分别为93.7%(出水0.3 mg·L^(-1)),87.5%(出水0.7mg·L^(-1)),98.2%(出水3mg·L^(-1)),86.9%(出水0.1mg·L^(-1))。当COD提高至450mg·L^(-1)时,氨氮去除率逐渐降低到40.7%,亚硝氮在COD为350mg·L^(-1)时去除率降低至22.5%。在整个系统运行过程中,变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)是反应器中的绝对优势类群。当COD由150mg·L^(-1)上升到350mg·L^(-1)时,变形菌门的相对丰度却由63.9%~75.2%提高到88.1%~92.4%;拟杆菌门的相对丰度则由11.9%~13.0%降低到4.5%~5.4%;引起污泥膨胀的发硫菌属(Thiothrix)的相对丰度由6.24%~7.08%增加到58.16%~76.74%,表明反应器污泥膨胀趋势加剧。应用A/O-MBBR工艺处理海水养殖废水时,在COD为150mg·L^(-1)时效果较好,随着COD浓度提高,引起污泥膨胀的微生物开始大量滋生。
基金This work was supported by the National Key Research and Development Program of China(No.2018 YFC1407601)the Start-up Foundation for Introducing Talent of NUIST and Guangxi Innovation Driven Development Project(major science and technology project).
文摘In order to explore the effect of carbon and nitrogen(C/N)ratio on the performance of anoxic/aerobic-moving bed bio-film reactor(A/O-MBBR)process for treating mariculture wastewater,a laboratory-scale A/O-MBBR was conducted.The results showed that the reduction of C/N ratio was conducive to improving the removal efficiency of chemical oxygen demand(COD)and ammonia nitrogen(NH_(4)^(+)-N),while inhibiting that of nitrite nitrogen(NO_(2)^(−)-N)and nitrate nitrogen(NO_(3)^(−)-N).The extracellular polymeric substances(EPS)in anoxic zone were significantly higher in concentration than that in aerobic zone although they both declined with decrease of C/N ratio.The result provides solid support for better controlling the pollution of mariculture wastewater.
基金the Fundamental Research Funds for the Central Universities of China(No.201964004)the National Natural Science Foundation of China(No.41977315).
文摘The wide application of antibiotics in aquaculture requires an efficient treatment of the wastewater before discharging it into the environment.During the wastewater treatment,the influence of antibiotics on the performance of bioreactor should be well revealed due to their toxicity to the functional microbial community.In this study,the effect of feeding 10-30 mg L−1 sulfamethoxazole(SMX)in influent on the performance of an anoxic/oxic-moving bed biofilm reactor(A/O-MBBR)treating mariculture wastewater and the responding change of biofilm microbial communities was investigated.The COD average removal rate remained at 94.61%-97.34%with the dosage of SMX.Compared with that,the nitrifying removals of NH4+-N and NO2−-N were violently inhibited by 30 mg L−1 SMX and denitrifying removal of the NO3−-N decreased obviously with 20 mg L−1 or more SMX.The microbial community in the successful startup bioreactor was relatively abundant,while the diversity of microbial community decreased with the increase of feeding SMX.The salt-tolerant and SMX-resistant genera Arcobacter,Thiothrix,Desulfuromusa and Nitrosomonas were gradually enriched and finally played a vital role in converting COD and recycling nitrogen and sulfur.Hence,the present A/O-MBBR reactor with the salt-tolerant functional microbiota achieved efficient removal of pollutants in the presence of low concentration(e.g.,10 mg L−1)SMX.
