DEM数据源及分辨率会影响流域特征参数的提取,进而影响水文模拟结果。将ASTER 30 m DEM、SRTM 90 m DEM及基于ASTER 30 m DEM的40 m、50 m、60 m、70 m、80 m、90 m重采样DEM作为HEC-geo HMS模型输入,提取流域特征,采用HEC-HMS模型,以...DEM数据源及分辨率会影响流域特征参数的提取,进而影响水文模拟结果。将ASTER 30 m DEM、SRTM 90 m DEM及基于ASTER 30 m DEM的40 m、50 m、60 m、70 m、80 m、90 m重采样DEM作为HEC-geo HMS模型输入,提取流域特征,采用HEC-HMS模型,以西笤溪流域为研究区域,分析2011年6月和2011年8—9月的两场降雨径流过程中,DEM数据源和分辨率对水文模拟输出的影响。研究结果表明,两次径流模拟结果与实测数据拟合都较好,模型确定性系数都大于0.82,但是单峰的洪水模拟效果总体更好,基于SRTM 90 m的模型确定性系数比基于ASTER 30 m DEM、重采样90 m DEM的模型确定性系数都大。基于重采样DEM的模型确定性系数变化较大,而且与分辨率的变化呈非线性关系。在HEC-HMS的模拟中,基于ASTER 30 m DEM和基于SRTM 90 m DEM的模拟输出结果相对误差相差3%~5%,基于SRTM 90 m DEM和基于重采样90 m DEM的模拟输出结果相对误差相差2%~4%,基于重采样DEM的模拟输出结果相对误差相差最大达到了11%。展开更多
<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels ...<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. Results: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1±3.2 and 243.0±21.6, respectively, while those after thawing were 23.2±2.5 and 105.7±28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. Conclusion: HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.展开更多
This study presents the numerical evaluation about the impact of flow disturbance near the intrados and extrados regions of the 90 degree bended elbow using CFX for several practical cases where the 90 degree bended u...This study presents the numerical evaluation about the impact of flow disturbance near the intrados and extrados regions of the 90 degree bended elbow using CFX for several practical cases where the 90 degree bended upward elbow is located in a proximity to the butterfly valve and the butterfly valve open angle is changed. For the change of a butterfly valve open angle from 60% to 100% and the increase of the distance between a valve and a 90 degree bended elbow, the effect of FAC (Flow-Accelerated Corrosion) in the 90 degree bended elbow may be neglected because the value and distribution of the velocity and shear stress is rapidly decreased comparing with the present status installed in an industry, and the data of 100% valve open (Case 3) and L/D ≈ 5 (Case 4) are very good agreement comparing with the reference data, L/D ≈ 8 (Case 2). The reasons are that flow already maintains a fully developed condition and a steady state in spite of less distance than the reference case, L/D = 8. Therefore, smooth flow fields have approached at a 90 degree bended elbow. Then, the effect of shear stress and vortex is hardly investigated around the intrados area of 90 degree bended elbow.展开更多
Objective To investigate the expression of glucocerticoid receptor (GR) and heat shock protein 90(HSP90) mRNA in peripheral blood mononuclear cells (PBMCs) from steroid-sensitive (SS), steroiddependent (SD) and stero...Objective To investigate the expression of glucocerticoid receptor (GR) and heat shock protein 90(HSP90) mRNA in peripheral blood mononuclear cells (PBMCs) from steroid-sensitive (SS), steroiddependent (SD) and steroid-resistant (SR) asthmatics patients, and to evaluate the role of GR and HSP90in the pathogenesis of SR.Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expressions of GR and HSP90 mRNA in PBMC stimulated with IL-2 and/or IL-4 from 10 normal volunteers,10 SS, 5 SD and 6 SR patients.Results The expressions of GR and HSP90 mRNA were the highest in PBMC from SR patients (0.730±0.171 and 1.122±0.165, respectively) compared with the normals (P＜0.05). The second was from SS patients (0.359±0.350 and 0.885±0.250, respectively). The lowest was from the SD patients (0.017±0.008 and 0.078 ± 0.039, respectively). The ratio of HSP90/GR in SR was significantly lower than that in the others, and it suggested that the expression of HSP90 mRNA was not sufficient in this group of patients. When PBMC from SS, SD and SR was incubated with IL-2 or IL-4 alone, no changes in GR and HSP90 mRNA expression were observed. While incubated with combination of IL-2 and IL-4, a significantly higher expression of GR mRNA was observed in all asthmatics, and a significantly higher expression of HSP90 mRNA was observed only in SS and SD patients.Conclusion The lowering of HSP90/GR ratio may be one of the causes of SR. IL-2 and IL-4 may play roles in the imbalance of HSP90/GR.展开更多
Severe fever with thrombocytopenia syndrome(SFTS)caused by the SFTS virus(SFTSV)is an emerging disease in East Asia with a fatality rate of up to 30%.However,the viral-host interaction of SFTSV remains largely unknown...Severe fever with thrombocytopenia syndrome(SFTS)caused by the SFTS virus(SFTSV)is an emerging disease in East Asia with a fatality rate of up to 30%.However,the viral-host interaction of SFTSV remains largely unknown.The heat-shock protein 90(Hsp90)family consists of highly conserved chaperones that fold and remodel proteins and has a broad impact on the infection of many viruses.Here,we showed that Hsp90 is an important host factor involved in SFTSV infection.Hsp90 inhibitors significantly reduced SFTSV replication,viral protein expression,and the formation of inclusion bodies consisting of nonstructural proteins(NSs).Among viral proteins,NSs appeared to be the most reduced when Hsp90 inhibitors were used,and further analysis showed that their translation was affected.Co-immunoprecipitation of NSs with four isomers of Hsp90 showed that Hsp90βspecifically interacted with them.Knockdown of Hsp90βexpression also inhibited replication of SFTSV.These results suggest that Hsp90βplays a critical role during SFTSV infection and could be a potential target for the development of drugs against SFTS.展开更多
文摘DEM数据源及分辨率会影响流域特征参数的提取,进而影响水文模拟结果。将ASTER 30 m DEM、SRTM 90 m DEM及基于ASTER 30 m DEM的40 m、50 m、60 m、70 m、80 m、90 m重采样DEM作为HEC-geo HMS模型输入,提取流域特征,采用HEC-HMS模型,以西笤溪流域为研究区域,分析2011年6月和2011年8—9月的两场降雨径流过程中,DEM数据源和分辨率对水文模拟输出的影响。研究结果表明,两次径流模拟结果与实测数据拟合都较好,模型确定性系数都大于0.82,但是单峰的洪水模拟效果总体更好,基于SRTM 90 m的模型确定性系数比基于ASTER 30 m DEM、重采样90 m DEM的模型确定性系数都大。基于重采样DEM的模型确定性系数变化较大,而且与分辨率的变化呈非线性关系。在HEC-HMS的模拟中,基于ASTER 30 m DEM和基于SRTM 90 m DEM的模拟输出结果相对误差相差3%~5%,基于SRTM 90 m DEM和基于重采样90 m DEM的模拟输出结果相对误差相差2%~4%,基于重采样DEM的模拟输出结果相对误差相差最大达到了11%。
文摘<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. Results: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1±3.2 and 243.0±21.6, respectively, while those after thawing were 23.2±2.5 and 105.7±28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. Conclusion: HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.
文摘This study presents the numerical evaluation about the impact of flow disturbance near the intrados and extrados regions of the 90 degree bended elbow using CFX for several practical cases where the 90 degree bended upward elbow is located in a proximity to the butterfly valve and the butterfly valve open angle is changed. For the change of a butterfly valve open angle from 60% to 100% and the increase of the distance between a valve and a 90 degree bended elbow, the effect of FAC (Flow-Accelerated Corrosion) in the 90 degree bended elbow may be neglected because the value and distribution of the velocity and shear stress is rapidly decreased comparing with the present status installed in an industry, and the data of 100% valve open (Case 3) and L/D ≈ 5 (Case 4) are very good agreement comparing with the reference data, L/D ≈ 8 (Case 2). The reasons are that flow already maintains a fully developed condition and a steady state in spite of less distance than the reference case, L/D = 8. Therefore, smooth flow fields have approached at a 90 degree bended elbow. Then, the effect of shear stress and vortex is hardly investigated around the intrados area of 90 degree bended elbow.
文摘Objective To investigate the expression of glucocerticoid receptor (GR) and heat shock protein 90(HSP90) mRNA in peripheral blood mononuclear cells (PBMCs) from steroid-sensitive (SS), steroiddependent (SD) and steroid-resistant (SR) asthmatics patients, and to evaluate the role of GR and HSP90in the pathogenesis of SR.Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expressions of GR and HSP90 mRNA in PBMC stimulated with IL-2 and/or IL-4 from 10 normal volunteers,10 SS, 5 SD and 6 SR patients.Results The expressions of GR and HSP90 mRNA were the highest in PBMC from SR patients (0.730±0.171 and 1.122±0.165, respectively) compared with the normals (P＜0.05). The second was from SS patients (0.359±0.350 and 0.885±0.250, respectively). The lowest was from the SD patients (0.017±0.008 and 0.078 ± 0.039, respectively). The ratio of HSP90/GR in SR was significantly lower than that in the others, and it suggested that the expression of HSP90 mRNA was not sufficient in this group of patients. When PBMC from SS, SD and SR was incubated with IL-2 or IL-4 alone, no changes in GR and HSP90 mRNA expression were observed. While incubated with combination of IL-2 and IL-4, a significantly higher expression of GR mRNA was observed in all asthmatics, and a significantly higher expression of HSP90 mRNA was observed only in SS and SD patients.Conclusion The lowering of HSP90/GR ratio may be one of the causes of SR. IL-2 and IL-4 may play roles in the imbalance of HSP90/GR.
基金supported by grants from the National Natural Science Foundation of China(31900146)the key Biosafety Science and Technology Program of Hubei Jiangxia Laboratory(JXBS001)+1 种基金the Hubei Natural Science Foundation for Distinguished Young Scholars(2021CFA050)the Creative Research Group Program of Natural Science Foundation of Hubei Province(2022CFA021).
文摘Severe fever with thrombocytopenia syndrome(SFTS)caused by the SFTS virus(SFTSV)is an emerging disease in East Asia with a fatality rate of up to 30%.However,the viral-host interaction of SFTSV remains largely unknown.The heat-shock protein 90(Hsp90)family consists of highly conserved chaperones that fold and remodel proteins and has a broad impact on the infection of many viruses.Here,we showed that Hsp90 is an important host factor involved in SFTSV infection.Hsp90 inhibitors significantly reduced SFTSV replication,viral protein expression,and the formation of inclusion bodies consisting of nonstructural proteins(NSs).Among viral proteins,NSs appeared to be the most reduced when Hsp90 inhibitors were used,and further analysis showed that their translation was affected.Co-immunoprecipitation of NSs with four isomers of Hsp90 showed that Hsp90βspecifically interacted with them.Knockdown of Hsp90βexpression also inhibited replication of SFTSV.These results suggest that Hsp90βplays a critical role during SFTSV infection and could be a potential target for the development of drugs against SFTS.
