利用偶发分枝杆菌(Mycobacterium fortuitum)CICC 10279发酵断甾醇侧链进行定向菌株筛选;对选出的具有较高甾醇降解活性的菌株,借助TLC法开展多批次生物降解实验过程研究;并重点对积累9α-羟基雄烯二酮(9α-OH-AD)化合物的菌株稳定性及...利用偶发分枝杆菌(Mycobacterium fortuitum)CICC 10279发酵断甾醇侧链进行定向菌株筛选;对选出的具有较高甾醇降解活性的菌株,借助TLC法开展多批次生物降解实验过程研究;并重点对积累9α-羟基雄烯二酮(9α-OH-AD)化合物的菌株稳定性及转化过程进行考察;在此基础上利用选出的22号菌株分别进行底物为胆固醇和植物甾醇的半微量制备实验.结果显示,分离的样品具有高纯度,HPLC分析为95.7%;TLC、HPLC、MS、1H N M R、13C N M R、i.r.等光谱数据分析结构确证其为9α-OH-A D;半微量制备实验以胆固醇或植物甾醇为底物,9α-OH-A D重量收得率分别可达34.0%和30.8%.本研究可能为高效含卤(氟,氯)皮质激素药物工业生产提供了一种很有用处的中间体.展开更多
Objective： To investigate the efficacy and mechanism of EGDT against NPC cell lines. Methods： M＇IT assay was used to assess cell proliferation inhibition of EGDT. The apoptotic induction and cell cycle arrest were ...Objective： To investigate the efficacy and mechanism of EGDT against NPC cell lines. Methods： M＇IT assay was used to assess cell proliferation inhibition of EGDT. The apoptotic induction and cell cycle arrest were detected by flow cytometry. Western blot was adopted to detect the protein levels. Quantitative Real-time PCR was used to determine the mRNA expressions. The NPC xenografts were established to evaluate the tumor growth inhibition of EGDT. Immunohistochemistry was applied to analyze the EGFR expression in the tumor tissues. Results： EGDT showed proliferation inhibition on the NPC cell, induced G0/G1 phase arrest and cell apop- tosis in vitro. EGDT decreased the protein and mRNA levels of EGFR and its downstream RAF/MEK/ERK and PI3K/AKT pathways in time- and dose-dependent manner. Furthermore, EGDT also showed a sound antitumnr activity in NPC xenograft in vivo. Conclusion： The treatment of EGDT displays EGFR and its mediated downstream signaling pathway block- ade through decreasing the protein and mRNA levels, suggesting a promising strategy in treating human NPC.展开更多
文摘利用偶发分枝杆菌(Mycobacterium fortuitum)CICC 10279发酵断甾醇侧链进行定向菌株筛选;对选出的具有较高甾醇降解活性的菌株,借助TLC法开展多批次生物降解实验过程研究;并重点对积累9α-羟基雄烯二酮(9α-OH-AD)化合物的菌株稳定性及转化过程进行考察;在此基础上利用选出的22号菌株分别进行底物为胆固醇和植物甾醇的半微量制备实验.结果显示,分离的样品具有高纯度,HPLC分析为95.7%;TLC、HPLC、MS、1H N M R、13C N M R、i.r.等光谱数据分析结构确证其为9α-OH-A D;半微量制备实验以胆固醇或植物甾醇为底物,9α-OH-A D重量收得率分别可达34.0%和30.8%.本研究可能为高效含卤(氟,氯)皮质激素药物工业生产提供了一种很有用处的中间体.
基金supported by the Youth Research Project of Health and Family Planning Commission of Fujian Province (2015-2-37)
文摘Objective： To investigate the efficacy and mechanism of EGDT against NPC cell lines. Methods： M＇IT assay was used to assess cell proliferation inhibition of EGDT. The apoptotic induction and cell cycle arrest were detected by flow cytometry. Western blot was adopted to detect the protein levels. Quantitative Real-time PCR was used to determine the mRNA expressions. The NPC xenografts were established to evaluate the tumor growth inhibition of EGDT. Immunohistochemistry was applied to analyze the EGFR expression in the tumor tissues. Results： EGDT showed proliferation inhibition on the NPC cell, induced G0/G1 phase arrest and cell apop- tosis in vitro. EGDT decreased the protein and mRNA levels of EGFR and its downstream RAF/MEK/ERK and PI3K/AKT pathways in time- and dose-dependent manner. Furthermore, EGDT also showed a sound antitumnr activity in NPC xenograft in vivo. Conclusion： The treatment of EGDT displays EGFR and its mediated downstream signaling pathway block- ade through decreasing the protein and mRNA levels, suggesting a promising strategy in treating human NPC.
