Fragrances are widely used in our daily life and have made great contributions to creating a clean and fresh healthy air environment. However, the rapid volatilization severely influences their storage,application and...Fragrances are widely used in our daily life and have made great contributions to creating a clean and fresh healthy air environment. However, the rapid volatilization severely influences their storage,application and quality. Therefore, it is necessary and urgent to develop approaches to controllably release the odorants as demanded. In this study, photo-driven mesoporous silica nanospheres loaded with sandela 803 was designed, prepared and named as S803@MS-S. S803@MS-S possessed ordered mesoporous, large specific surface area and pore volume. After adsorption of sandela 803, the S803@MSS was added into wallpaper to prepare fragrant wallpaper S803@MS-S-W. And this wallpaper exhibited excellent sustained and controlled release performances stimulated by light.展开更多
AIM To demonstrate whether bcl 2 gene can affect or alter biological behavior of a stomach carcinoma cell line MGC 803. METHODS To transduct a retrovirus containing bcl 2 antisense RNA to MGC 803 cells ...AIM To demonstrate whether bcl 2 gene can affect or alter biological behavior of a stomach carcinoma cell line MGC 803. METHODS To transduct a retrovirus containing bcl 2 antisense RNA to MGC 803 cells and then to analyse the Bcl 2 protein expression in the cells by Western blotting. To observe the morphology alteration, detect the G1 phase arrest by FCM, inhibition of proliferation by MTT method and tumorigenicity in nude mice. RESULTS The MGC anti bcl 2 cells shows contact inhibition, morphological alteration, from round or near round to shuttle like, decelerated growth rate, G1 phase arrest and weakened tumorigenicity in nude mice unlike the control (MGC neo cells). CONCLUSION Antisense RNA to bcl 2, not only can induce apoptosis, but also reverse the biological behavior of MGC 803 cells. This would be a potential application to the gene therapy for stomach cancers.展开更多
We first obtained by impregnating the zeolite with salts U, W and V and examined the X-ray uranium catalyst SAPO-U, W, V based on natural zeolite Chankanayskogoe deposits for the oxidation of SO2 to SO3.
本文研究青钱柳多糖(polysaccharides isolated from Cyclocarya paliurus(Batal.)Iljinskjk,PCP)对人胃癌MGC_803细胞生长的影响。采用噻唑蓝(MTT)法检测PCP对MGC_803细胞生长的影响;Annexin法检测PCP对MGC803细胞诱导凋亡的作用。实...本文研究青钱柳多糖(polysaccharides isolated from Cyclocarya paliurus(Batal.)Iljinskjk,PCP)对人胃癌MGC_803细胞生长的影响。采用噻唑蓝(MTT)法检测PCP对MGC_803细胞生长的影响;Annexin法检测PCP对MGC803细胞诱导凋亡的作用。实验结果表明PCP在50、100、200、400μg/mL浓度条件下,均可极显著性抑制人胃癌MGC_803细胞生长(P<0.01),抑制率可达65.07%,细胞凋亡率可达25.44%。说明青钱柳多糖具有抑制人胃癌MGC803细胞生长的生物活性。展开更多
AIM: To determine the role of p38 MAP kinase signal transduction pathways in diallyl disulfide (DADS)-induced G2/M arrest in human gastric cancer MGC803 cells.METHODS: MGC803 cell growth inhibition was measured by MTT...AIM: To determine the role of p38 MAP kinase signal transduction pathways in diallyl disulfide (DADS)-induced G2/M arrest in human gastric cancer MGC803 cells.METHODS: MGC803 cell growth inhibition was measured by MTT assay. Phase distribution of cell cyde was analyzed by flow cytometry. Expression of Cdc25C, p38, phosphorylation of p38 (pp38) were determined by Western blotting.RESULTS: MT1- assay showed that SB203580, a specific p38 MAPK inhibitor blocked DADS-induced growth inhibition.Flow cytometry analysis revealed that treatment of MGC803 cells with 30 mg/L DADS increased the percentage of cells in the G2/M phase from 9.3% to 39.4% (P<0.05), whereas inhibition of p38 activity by SB203580 abolished induction of G2/M arrest by DADS. Western blotting showed that phosphorylation of p38 was increased 3.52-fold following treatment of MGC803 cells with 30 mg/L DADS for 20 min(P<0.05), whereas Cdc25C was decreased 68% following treatment of MGCS03 cells with 30 mg/L DADS for 24 h(P<0.05). Decreased Cdc25C protein expression by DADS was attenuated by SB203580 (P<0.05).CONCLUSION: DADS-induced G2/M arrest of MGC803 cells involves activation of p38 MAP kinase pathways.Decreased Cdc25C protein expression by p38 MAPK played a crucial role in G2/M arrest after treatment with DADS.展开更多
基金financially supported by the National High Technology Research and Development Program (No. 2016YFA0200303)the Beijing Natural Science Foundation (No. L172046)+2 种基金the National Natural Science Foundation of China(Nos. 31522023, 51373177 and 51573188)the Beijing Municipal Science & Technology Commission (No. Z161100002616015)the "Strategic Priority Research Program Research Program" of the Chinese Academy of Sciences(No. XDA09030301-3)
文摘Fragrances are widely used in our daily life and have made great contributions to creating a clean and fresh healthy air environment. However, the rapid volatilization severely influences their storage,application and quality. Therefore, it is necessary and urgent to develop approaches to controllably release the odorants as demanded. In this study, photo-driven mesoporous silica nanospheres loaded with sandela 803 was designed, prepared and named as S803@MS-S. S803@MS-S possessed ordered mesoporous, large specific surface area and pore volume. After adsorption of sandela 803, the S803@MSS was added into wallpaper to prepare fragrant wallpaper S803@MS-S-W. And this wallpaper exhibited excellent sustained and controlled release performances stimulated by light.
文摘AIM To demonstrate whether bcl 2 gene can affect or alter biological behavior of a stomach carcinoma cell line MGC 803. METHODS To transduct a retrovirus containing bcl 2 antisense RNA to MGC 803 cells and then to analyse the Bcl 2 protein expression in the cells by Western blotting. To observe the morphology alteration, detect the G1 phase arrest by FCM, inhibition of proliferation by MTT method and tumorigenicity in nude mice. RESULTS The MGC anti bcl 2 cells shows contact inhibition, morphological alteration, from round or near round to shuttle like, decelerated growth rate, G1 phase arrest and weakened tumorigenicity in nude mice unlike the control (MGC neo cells). CONCLUSION Antisense RNA to bcl 2, not only can induce apoptosis, but also reverse the biological behavior of MGC 803 cells. This would be a potential application to the gene therapy for stomach cancers.
文摘We first obtained by impregnating the zeolite with salts U, W and V and examined the X-ray uranium catalyst SAPO-U, W, V based on natural zeolite Chankanayskogoe deposits for the oxidation of SO2 to SO3.
文摘本文研究青钱柳多糖(polysaccharides isolated from Cyclocarya paliurus(Batal.)Iljinskjk,PCP)对人胃癌MGC_803细胞生长的影响。采用噻唑蓝(MTT)法检测PCP对MGC_803细胞生长的影响;Annexin法检测PCP对MGC803细胞诱导凋亡的作用。实验结果表明PCP在50、100、200、400μg/mL浓度条件下,均可极显著性抑制人胃癌MGC_803细胞生长(P<0.01),抑制率可达65.07%,细胞凋亡率可达25.44%。说明青钱柳多糖具有抑制人胃癌MGC803细胞生长的生物活性。
基金Supported by the Natural Science Foundation of Hunan Province,No.02JJY2026,01JJY2146 and the Foundation of Hunan ProvinceEducation Department,No.01A016
文摘AIM: To determine the role of p38 MAP kinase signal transduction pathways in diallyl disulfide (DADS)-induced G2/M arrest in human gastric cancer MGC803 cells.METHODS: MGC803 cell growth inhibition was measured by MTT assay. Phase distribution of cell cyde was analyzed by flow cytometry. Expression of Cdc25C, p38, phosphorylation of p38 (pp38) were determined by Western blotting.RESULTS: MT1- assay showed that SB203580, a specific p38 MAPK inhibitor blocked DADS-induced growth inhibition.Flow cytometry analysis revealed that treatment of MGC803 cells with 30 mg/L DADS increased the percentage of cells in the G2/M phase from 9.3% to 39.4% (P<0.05), whereas inhibition of p38 activity by SB203580 abolished induction of G2/M arrest by DADS. Western blotting showed that phosphorylation of p38 was increased 3.52-fold following treatment of MGC803 cells with 30 mg/L DADS for 20 min(P<0.05), whereas Cdc25C was decreased 68% following treatment of MGCS03 cells with 30 mg/L DADS for 24 h(P<0.05). Decreased Cdc25C protein expression by DADS was attenuated by SB203580 (P<0.05).CONCLUSION: DADS-induced G2/M arrest of MGC803 cells involves activation of p38 MAP kinase pathways.Decreased Cdc25C protein expression by p38 MAPK played a crucial role in G2/M arrest after treatment with DADS.
