为提高电信网设备应对异常信令访问的检测能力,需对64K信令进行分析并处理。为了提高解析效率并满足近年来相关产品对自主可控越来越高的要求,设计了一种基于国产现场可编程门阵列(Field Programmable Gate Array, FPGA)的信令解析方案...为提高电信网设备应对异常信令访问的检测能力,需对64K信令进行分析并处理。为了提高解析效率并满足近年来相关产品对自主可控越来越高的要求,设计了一种基于国产现场可编程门阵列(Field Programmable Gate Array, FPGA)的信令解析方案,给出了方案的总体设计思路,并对FPGA实现的功能模块进行详细说明。对系统进行设计时,采用模块化参数化方法以及在关键环节添加状态参数,提高了可扩展性并可以对模块内部运行状态进行监控,最终实现了对信令高效且灵活的解析,主要器件等均为国产。经过测试,可以实现STM-1(STM-Synchronous Transfer Module-1)数据的接入、串并转换、HDLC(High-level Data Link Control)解帧等功能,完成32路64K信令的并发处理,模块运行状态可查可看,达到了预期的效果。以STM-1为例,基于现有功能的模块化设计,可以平滑地扩展到STM-4、STM-16的应用。展开更多
AIM： To construct two kinds of anti-gastrin immunogen based on P64K protein from Neisseria rneningitids and to compare their immunogenic effect. METHODS： G17P64K gene was cloned and ligated into pET28a plasmid, then...AIM： To construct two kinds of anti-gastrin immunogen based on P64K protein from Neisseria rneningitids and to compare their immunogenic effect. METHODS： G17P64K gene was cloned and ligated into pET28a plasmid, then transformed into BL21（DE3）. After inoculation of LB medium and IPTG induction, the recombinant protein was solubly expressed at a high level. The purification of G17P64K fusion protein was similar to that of P64K. An initial step of purification consisting of 30% saturated ammonium sulfate precipitation was done, Additional fine optimizations included phenyl-sepharose, G200 Sephadex gel filtration and Q-sepharose anion exchanger chromatography. Highly purified protein was obtained and sequenced at the N-terminal amino acid residues. Polypeptide was synthesized by Fmoc solid phase chemical method and cross-linked to carrier protein P64K and DT mutant by MBS method and then the rabbit anti-gastrin 17 antibody was prepared by immunizing rabbit with cross-linked and fused protein. The titer and the activity in vitro of antibody were assessed. RESULTS： G17P64K gene and the recombinant bacteria were obtained. After four steps purification, protein sample that has the purity above 90% was achieved. At the 84^th day after the first immunization, the titer of antibody against cross-linked protein reached 51 200. Evaluation of the antibody in vitro manifested that it had a high inhibitory activity on the growth of tumor cell SW480. CONCLUSION： The P64K-polypeptide cross-linked immunogen immunized rabbit and achieved a higher titer antibody against gastrin 17 than the G17P64K fusion protein immunogen, which could inhibit the growth of the tumor cell SW480.展开更多
目的表达并纯化重组脑膜炎球菌P64K蛋白，观察其载体作用。方法利用基因工程技术在大肠杆菌中表达P64K蛋白，用硫酸铵盐析沉淀和两步层析纯化P64K蛋白。以重组P64K蛋白为载体蛋白，以已二酰肼为连接剂，在碳化二亚胺的作用下，将P64K蛋...目的表达并纯化重组脑膜炎球菌P64K蛋白，观察其载体作用。方法利用基因工程技术在大肠杆菌中表达P64K蛋白，用硫酸铵盐析沉淀和两步层析纯化P64K蛋白。以重组P64K蛋白为载体蛋白，以已二酰肼为连接剂，在碳化二亚胺的作用下，将P64K蛋白与活化的A群脑膜炎球菌多糖（group A meningococcal polysaccharide, GAMP）结合，制备GAMP-P64K结合物。免疫BALB／c小鼠，间接ELISA法测定血清特异性抗P64K蛋白和GAMPIgG抗体，分析P64K蛋白的免疫原性。结果重组P64K蛋白在大肠杆菌中主要以可溶性形式表达，表达量约占菌体总蛋白的35％。动物实验证明，重组P64K蛋白具有较强的免疫原性，表明P64K蛋白具有载体蛋白作用。结论在大肠杆菌中成功表达了重组P64K蛋白，以重组P64K蛋白为载体制备的GAMP—P64K结合物具有良好的免疫原性。这为以重组P64K蛋白为蛋白载体制备其他结合疫苗奠定了实验基础。展开更多
文摘为提高电信网设备应对异常信令访问的检测能力,需对64K信令进行分析并处理。为了提高解析效率并满足近年来相关产品对自主可控越来越高的要求,设计了一种基于国产现场可编程门阵列(Field Programmable Gate Array, FPGA)的信令解析方案,给出了方案的总体设计思路,并对FPGA实现的功能模块进行详细说明。对系统进行设计时,采用模块化参数化方法以及在关键环节添加状态参数,提高了可扩展性并可以对模块内部运行状态进行监控,最终实现了对信令高效且灵活的解析,主要器件等均为国产。经过测试,可以实现STM-1(STM-Synchronous Transfer Module-1)数据的接入、串并转换、HDLC(High-level Data Link Control)解帧等功能,完成32路64K信令的并发处理,模块运行状态可查可看,达到了预期的效果。以STM-1为例,基于现有功能的模块化设计,可以平滑地扩展到STM-4、STM-16的应用。
基金Supported by Grants from National High Technology Research and Development Program, No.2002AA2Z345B and No.2004AA2Z3803 of the Ministry of Science and Technology of China
文摘AIM： To construct two kinds of anti-gastrin immunogen based on P64K protein from Neisseria rneningitids and to compare their immunogenic effect. METHODS： G17P64K gene was cloned and ligated into pET28a plasmid, then transformed into BL21（DE3）. After inoculation of LB medium and IPTG induction, the recombinant protein was solubly expressed at a high level. The purification of G17P64K fusion protein was similar to that of P64K. An initial step of purification consisting of 30% saturated ammonium sulfate precipitation was done, Additional fine optimizations included phenyl-sepharose, G200 Sephadex gel filtration and Q-sepharose anion exchanger chromatography. Highly purified protein was obtained and sequenced at the N-terminal amino acid residues. Polypeptide was synthesized by Fmoc solid phase chemical method and cross-linked to carrier protein P64K and DT mutant by MBS method and then the rabbit anti-gastrin 17 antibody was prepared by immunizing rabbit with cross-linked and fused protein. The titer and the activity in vitro of antibody were assessed. RESULTS： G17P64K gene and the recombinant bacteria were obtained. After four steps purification, protein sample that has the purity above 90% was achieved. At the 84^th day after the first immunization, the titer of antibody against cross-linked protein reached 51 200. Evaluation of the antibody in vitro manifested that it had a high inhibitory activity on the growth of tumor cell SW480. CONCLUSION： The P64K-polypeptide cross-linked immunogen immunized rabbit and achieved a higher titer antibody against gastrin 17 than the G17P64K fusion protein immunogen, which could inhibit the growth of the tumor cell SW480.
文摘目的表达并纯化重组脑膜炎球菌P64K蛋白，观察其载体作用。方法利用基因工程技术在大肠杆菌中表达P64K蛋白，用硫酸铵盐析沉淀和两步层析纯化P64K蛋白。以重组P64K蛋白为载体蛋白，以已二酰肼为连接剂，在碳化二亚胺的作用下，将P64K蛋白与活化的A群脑膜炎球菌多糖（group A meningococcal polysaccharide, GAMP）结合，制备GAMP-P64K结合物。免疫BALB／c小鼠，间接ELISA法测定血清特异性抗P64K蛋白和GAMPIgG抗体，分析P64K蛋白的免疫原性。结果重组P64K蛋白在大肠杆菌中主要以可溶性形式表达，表达量约占菌体总蛋白的35％。动物实验证明，重组P64K蛋白具有较强的免疫原性，表明P64K蛋白具有载体蛋白作用。结论在大肠杆菌中成功表达了重组P64K蛋白，以重组P64K蛋白为载体制备的GAMP—P64K结合物具有良好的免疫原性。这为以重组P64K蛋白为蛋白载体制备其他结合疫苗奠定了实验基础。
