In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Z...In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Zn 2+ containing and Zn 2+ free system. Preliminary crystallographic analyses show that they all belong to T 3R 3 structural type. The results indicate that, except phenolic derivatives and lyotropic anions reported before, some other appropriate organic molecules can also induce the T 3R 3 conformation transition of insulin.展开更多
目的:探索白细胞介素(interleukin,IL)-6在胃癌中的作用及相关的分子机制。方法:50 ng/m L IL-6刺激胃癌细胞MGC-803后,利用MTT法检测细胞增殖、划痕实验检测细胞迁移的变化,RT-qPCR实验检测E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadhe...目的:探索白细胞介素(interleukin,IL)-6在胃癌中的作用及相关的分子机制。方法:50 ng/m L IL-6刺激胃癌细胞MGC-803后,利用MTT法检测细胞增殖、划痕实验检测细胞迁移的变化,RT-qPCR实验检测E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(vimentin)、锌指转录因子Snail1和miR-152在mRNA水平的表达,Western印迹检测E-cadherin,N-cadherin,vimentin,Snail1在蛋白水平的表达;IL-6与miR-152模拟物(miR-152 mimics)单独处理或者共同处理MGC-803细胞后,RT-qPCR检测PIK3R3在mRNA水平的表达,Western印迹检测PIK3R3、蛋白激酶B(Akt)和磷酸化-Akt(p-Akt)在蛋白水平的表达。结果:外源性IL-6明显促进MGC-803细胞的增殖与迁移(P<0.05),降低E-cadherin蛋白和mRNA和miR-152 mRNA的表达(P<0.01),增加N-cadherin,vimentin,Snail1,PIK3R3蛋白和mRNA及p-Akt蛋白的表达(P<0.05)。转染miR-152 mimics后明显降低了PIK3R3和p-Akt蛋白水平的表达(P<0.01)。同时,过表达miR-152能够降低IL-6诱导的PIK3R3及p-Akt的表达水平(P<0.01)。各组中Akt的表达均无明显变化(P>0.05)。结论:IL-6可能是通过下调miR-152表达,诱导PIK3R3表达,激活PI3K/Akt信号通路,从而促进胃癌细胞的增殖、迁移和上皮-间质转化。展开更多
Objective:To study the correlation of c-Myc and PIK3R3 expression with invasion and migration genes in melanoma tissue.Methods: Surgical removed melanoma tissue and normal skin tissue left in full-thickness skin graft...Objective:To study the correlation of c-Myc and PIK3R3 expression with invasion and migration genes in melanoma tissue.Methods: Surgical removed melanoma tissue and normal skin tissue left in full-thickness skin grafting in 95 Clinical Department, Fuzhou General Hospital between May 2015 and March 2017 were selected, the RNA in the tissue was separated and extracted, and then fluorescence quantitative PCR kit was used to determine the mRNA expression of c-Myc and PIK3R3 as well as invasion and migration genes.Results:c-Myc, PIK3R3, CXCL12, CXCR4, CXCR7, NRP1, MMP2, MMP9 and CatK mRNA expression in melanoma tissue were significantly higher than those in normal skin tissue while E-cadherin, TIMP1 and KISS1 mRNA expression were significantly lower than those in normal skin tissue;c-Myc mRNA expression in melanoma tissue was positively correlated with PIK3R3 mRNA expression;CXCL12, CXCR4, CXCR7, NRP1, MMP2, MMP9 and CatK mRNA expression in melanoma tissue with higher c-Myc expression were significantly higher than those in melanoma tissue with lower c-Myc expression while E-cadherin, TIMP1 and KISS1 mRNA expression were significantly lower than those in melanoma tissue with lower c-Myc expression.Conclusion:The high expression of c-Myc and PIK3R3 in melanoma tissue can promote the invasion and migration of cancer cells.展开更多
文摘In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Zn 2+ containing and Zn 2+ free system. Preliminary crystallographic analyses show that they all belong to T 3R 3 structural type. The results indicate that, except phenolic derivatives and lyotropic anions reported before, some other appropriate organic molecules can also induce the T 3R 3 conformation transition of insulin.
文摘Objective:To study the correlation of c-Myc and PIK3R3 expression with invasion and migration genes in melanoma tissue.Methods: Surgical removed melanoma tissue and normal skin tissue left in full-thickness skin grafting in 95 Clinical Department, Fuzhou General Hospital between May 2015 and March 2017 were selected, the RNA in the tissue was separated and extracted, and then fluorescence quantitative PCR kit was used to determine the mRNA expression of c-Myc and PIK3R3 as well as invasion and migration genes.Results:c-Myc, PIK3R3, CXCL12, CXCR4, CXCR7, NRP1, MMP2, MMP9 and CatK mRNA expression in melanoma tissue were significantly higher than those in normal skin tissue while E-cadherin, TIMP1 and KISS1 mRNA expression were significantly lower than those in normal skin tissue;c-Myc mRNA expression in melanoma tissue was positively correlated with PIK3R3 mRNA expression;CXCL12, CXCR4, CXCR7, NRP1, MMP2, MMP9 and CatK mRNA expression in melanoma tissue with higher c-Myc expression were significantly higher than those in melanoma tissue with lower c-Myc expression while E-cadherin, TIMP1 and KISS1 mRNA expression were significantly lower than those in melanoma tissue with lower c-Myc expression.Conclusion:The high expression of c-Myc and PIK3R3 in melanoma tissue can promote the invasion and migration of cancer cells.
