Purpose: To evaluate outcome and toxicity after carbon ion radiotherapy (RT) in skin carcinomas. Patients and Methods: Between November 2006 to September 2008,
Sugarcane(Saccharum officinarum)is one of the oldest crops cultivated by mankind.Numerous fungal taxa have been reported from this host,although most were not identified beyond genus level.In this study,we explored 31...Sugarcane(Saccharum officinarum)is one of the oldest crops cultivated by mankind.Numerous fungal taxa have been reported from this host,although most were not identified beyond genus level.In this study,we explored 31 sampling sites in Guangxi and Guangdong Provinces(China),and collected 370 diseased samples from leaves and roots of sugarcane,from which 762 strains were isolated.Our preliminary analysis based on internal transcribed spacer(ITS)sequences suggested that these isolates belonged to 143 species in 51 genera,but we could not assign 129 strains.Bipolaris,Chaetomium,Curvularia,Phoma and Nigrospora represented the top five most common genera identified,while 27 rare genera comprised only one identified species.In this study,we chose above mentioned common genera for in-depth morphological observations and multi-locus analyses,in order to identify these strains to species level.In this paper,we described one new genus,32 new species,and reported 19 new records for China and the asexual morph of Chaetomium olivaceum.Hitherto,this is the most comprehensive study with molecular identification and illustration of fungi associated with sugarcane,which greatly improves our understanding of culturable mycota associated with this host.展开更多
We experimentally demonstrated a diode-pumped Kerr-lens mode-locked femtosecond laser with a Yb:CaYAlO4 crystal as the gain medium. Pulse duration as short as 33 fs was obtained directly from the oscillator at a repet...We experimentally demonstrated a diode-pumped Kerr-lens mode-locked femtosecond laser with a Yb:CaYAlO4 crystal as the gain medium. Pulse duration as short as 33 fs was obtained directly from the oscillator at a repetition rate of 115 MHz. The central wavelength was at 1059 nm with a spectral bandwidth of 49 nm. These are, to the bestof our knowledge, the shortest pulses generated from a Yb:CaYAlO4 oscillator.展开更多
Based on test data from the hot forge experiments on Gleeble 1500, a Kumar type constitutive equation for 33Mn2V steel is established. Applying this constitutive equation in commercial FEM software of MSC/SuperForm 20...Based on test data from the hot forge experiments on Gleeble 1500, a Kumar type constitutive equation for 33Mn2V steel is established. Applying this constitutive equation in commercial FEM software of MSC/SuperForm 2005, the piercing process of 33Mn2V steel in Mannesmann mill is then simulated. The modeling results visualized the dynamic evolution of equivalent stress, especially inside the workpieee. It is shown that the non-uniform distribu- tion of stress on the internal and external surface of the workpiece is a distinct characteristic of processing tube pierc- ing. The numerical model was verified by comparing the values of calculated force parameters of the piercing process with those measured in laboratory eonditions. And it shows that the Kumar-type constitutive relationship meets the practical needs.展开更多
The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. I...The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. It was found that the temperature of semi-inactivation of oxygen evolution in the complex increased from 40.0 to about 43.0℃ in the presence of DGDG with 5-min heat treatment in the dark. Furthermore, when PS Ⅱ core complex was incubated for 5 min at 45.0℃, the oxygen evolution in the complex was completely lost, whilst the DGDG-complexed PS Ⅱ core complex still retained a 16% of activity (100% for 25.0℃). In addition, a 1-h incubation at 38.0℃ inactivated absolutely the oxygen evolution for the PS Ⅱ core complex. By contrast, there remained about 20% of activity (zero time for 100%) for the complex in the presence of DGDG under the same condition. These results indicate a new role of DGDG in the protection of PS Ⅱ core complex against the deleterious effects of temperature. It was展开更多
The 33 ku protein, prepared from NaCI-treated PSII particles, has shown an single band by SDS-PAGE. After being dialyzed against the low-osmotic medium at 4癈, it has been found that the 33 ku protein degraded into se...The 33 ku protein, prepared from NaCI-treated PSII particles, has shown an single band by SDS-PAGE. After being dialyzed against the low-osmotic medium at 4癈, it has been found that the 33 ku protein degraded into several small fragments. This result suggests that the preparations of 33 ku protein probably contain some latent proteinases. it has also been found, by the 2-D electrophoresis and IEF, that the preparations of 33 ku protein not dialyzed against the low-osmotic medium contain several polypeptides with nearly the same molecular weight but not the same isoelectric point as the 33 ku protein.展开更多
Human cyclophilin 33 (hCyP33), found in 1996, consists of an RNA-binding domain in N-terminus, a cyclophilin domain in C-terminus and a connected part between the two domains. RNA-binding proteins concern functions, s...Human cyclophilin 33 (hCyP33), found in 1996, consists of an RNA-binding domain in N-terminus, a cyclophilin domain in C-terminus and a connected part between the two domains. RNA-binding proteins concern functions, such as splicing, modification and transport, after transcription in eukaryotic cells. Cyclophilins (CyPs) possess enzymatic activity, namely peptidyl-proryl cis-trans isomerase (PPIase). They are involved in folding, transport and interaction of proteins. Cyclosporin A (CsA), an immunosuppressant used by organ transplantation, binds to CyPs and suppresses their enzymatic activity. However, up to now it is unknown that which cellular and physiological roles hCyP33, which possesses the above-mentioned both functions, plays. In this paper the binding specificity of hCyP33 to different cellular RNA is investigated by means of ion-exchange chromatography and affinity adsorption. The results show that it binds specifically to poly(A) tailed mRNA, namely poly(A)+RNA.展开更多
AIM: To investigate the expression of interleukin(IL)-33 in the cornea and human corneal epithelial cells(HCECs) exposed to Aspergillus fumigatus(A. fumigatus), and to determine the function of IL-33/ST2/p38 signaling...AIM: To investigate the expression of interleukin(IL)-33 in the cornea and human corneal epithelial cells(HCECs) exposed to Aspergillus fumigatus(A. fumigatus), and to determine the function of IL-33/ST2/p38 signaling pathway in the immune response of corneal epithelial cells to A. fumigatus infection.METHODS: The mRNA and protein expression of IL-33 in HCECs and mice corneas were examined by quantitative real-time reverse transcription polymerase chain reaction(q RT-PCR) and Western blot analysis, respectively. IL-33 expression was also detected in cornea samples from healthy donors and patients with fungal keratitis with immunohistochemistry. The cultured HCECs were treated with inactive A. fumigatus hyphae at various concentrations with or without recombinant human IL-33 protein, soluble recombinant ST2 protein, specific ST2 neutralizing antibody, or the mitogen-activated protein kinase(MAPK) p38 inhibitor SB203580 for evaluation of the expression and activation of IL-33/ST2/p38 signaling in the regulation of proinflammatory cytokines. The production levels of IL-6 and IL-1β were determined by qR T-PCR and enzymelinked immunosorbent assay(ELISA). The proliferation of HCECs was determined by a Cell Counting Kit-8(CCK8) assay and cell count.RESULTS: IL-33 expression levels increased in the corneal tissues of patients with fungal keratitis and in mice corneas of experimental A. fumigatus infection,as well as in HCECs with infection of A. fumigatus. A. fumigatus strongly stimulated HCECs-generated proinflammatory cytokine(IL-6 and IL-1β) production at both the mRNA and protein levels. This production of proinflammatory mediators stimulated by A. fumigatus was further stimulated by IL-33 and was prevented by soluble ST2 protein or ST2 neutralizing antibody. Moreover, IL-33 naturally promoted the p38 phosphorylation induced by A. fumigatus, which was suppressed by soluble ST2 protein. The MAPK p38 inhibitor SB203580 also inhibited the A. fumigatus-induced proinflammatory cytokine production. IL-33 administration f展开更多
目的探讨脑血管疾病患者血清中白细胞介素33(IL-33)水平与颈动脉粥样硬化斑块稳定性及脑梗死的关系及其作用机制。方法采用ELISA方法检测脑梗死组(n=63)、短暂性脑缺血发作组(n=30)及正常对照组(n=15)血清中IL-33的表达水平;采用Western...目的探讨脑血管疾病患者血清中白细胞介素33(IL-33)水平与颈动脉粥样硬化斑块稳定性及脑梗死的关系及其作用机制。方法采用ELISA方法检测脑梗死组(n=63)、短暂性脑缺血发作组(n=30)及正常对照组(n=15)血清中IL-33的表达水平;采用Western blotting、荧光定量PCR(qRT-PCR)和ELISA法检测体外培养的人外周血单个核细胞(PBMC)和U937细胞在接受外源性IL-33刺激后,MMP-9和Cox-2的表达量变化。结果脑梗死组IL-33的表达水平低于对照组和短暂性脑缺血发作组(P均<0.01);脑梗死组中不稳定斑块、稳定斑块和无斑块患者血清中IL-33的表达水平逐渐降低(P<0.01);脑梗死组中,与无糖尿病和高血脂症的患者相比,合并糖尿病和高脂血症的患者血清中IL-33水平降低(P均<0.01);外源性IL-33刺激可下调外周血单核细胞和U937细胞中M M P-9和Cox-2的表达和分泌量。结论 IL-33可被作为预测脑梗死及不稳定性粥样硬化斑块的潜在的血清标志物和治疗靶点。展开更多
文摘Purpose: To evaluate outcome and toxicity after carbon ion radiotherapy (RT) in skin carcinomas. Patients and Methods: Between November 2006 to September 2008,
基金partly supported by NSFC 31725001he China-Thailand Joint Lab on Microbial Biotechnology(MOST KY201701011).
文摘Sugarcane(Saccharum officinarum)is one of the oldest crops cultivated by mankind.Numerous fungal taxa have been reported from this host,although most were not identified beyond genus level.In this study,we explored 31 sampling sites in Guangxi and Guangdong Provinces(China),and collected 370 diseased samples from leaves and roots of sugarcane,from which 762 strains were isolated.Our preliminary analysis based on internal transcribed spacer(ITS)sequences suggested that these isolates belonged to 143 species in 51 genera,but we could not assign 129 strains.Bipolaris,Chaetomium,Curvularia,Phoma and Nigrospora represented the top five most common genera identified,while 27 rare genera comprised only one identified species.In this study,we chose above mentioned common genera for in-depth morphological observations and multi-locus analyses,in order to identify these strains to species level.In this paper,we described one new genus,32 new species,and reported 19 new records for China and the asexual morph of Chaetomium olivaceum.Hitherto,this is the most comprehensive study with molecular identification and illustration of fungi associated with sugarcane,which greatly improves our understanding of culturable mycota associated with this host.
基金supported by the National Major Scientific Instruments Development Project of China (Grant No. 2012YQ120047)the National Natural Science Foundation of China (Grant No. 61205130)
文摘We experimentally demonstrated a diode-pumped Kerr-lens mode-locked femtosecond laser with a Yb:CaYAlO4 crystal as the gain medium. Pulse duration as short as 33 fs was obtained directly from the oscillator at a repetition rate of 115 MHz. The central wavelength was at 1059 nm with a spectral bandwidth of 49 nm. These are, to the bestof our knowledge, the shortest pulses generated from a Yb:CaYAlO4 oscillator.
基金Item Sponsored by Tianjin Natural Science Foundation of China(06YFJ MJC02200,11JCZDJC22600)
文摘Based on test data from the hot forge experiments on Gleeble 1500, a Kumar type constitutive equation for 33Mn2V steel is established. Applying this constitutive equation in commercial FEM software of MSC/SuperForm 2005, the piercing process of 33Mn2V steel in Mannesmann mill is then simulated. The modeling results visualized the dynamic evolution of equivalent stress, especially inside the workpieee. It is shown that the non-uniform distribu- tion of stress on the internal and external surface of the workpiece is a distinct characteristic of processing tube pierc- ing. The numerical model was verified by comparing the values of calculated force parameters of the piercing process with those measured in laboratory eonditions. And it shows that the Kumar-type constitutive relationship meets the practical needs.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39890390)the State Basic Research Development Plan of China (Grant No. G1998010100)the Innovative Foundation of Laboratory of Photosynthesis Basic Research, In
文摘The influence of digalactosyldiacylglycerol (DGDG), one of the photosynthetic membrane lipids, on heat inactivation of the process of oxygen evolution has been studied in vitro in photosystem Ⅱ(PS Ⅱ) core complex. It was found that the temperature of semi-inactivation of oxygen evolution in the complex increased from 40.0 to about 43.0℃ in the presence of DGDG with 5-min heat treatment in the dark. Furthermore, when PS Ⅱ core complex was incubated for 5 min at 45.0℃, the oxygen evolution in the complex was completely lost, whilst the DGDG-complexed PS Ⅱ core complex still retained a 16% of activity (100% for 25.0℃). In addition, a 1-h incubation at 38.0℃ inactivated absolutely the oxygen evolution for the PS Ⅱ core complex. By contrast, there remained about 20% of activity (zero time for 100%) for the complex in the presence of DGDG under the same condition. These results indicate a new role of DGDG in the protection of PS Ⅱ core complex against the deleterious effects of temperature. It was
文摘The 33 ku protein, prepared from NaCI-treated PSII particles, has shown an single band by SDS-PAGE. After being dialyzed against the low-osmotic medium at 4癈, it has been found that the 33 ku protein degraded into several small fragments. This result suggests that the preparations of 33 ku protein probably contain some latent proteinases. it has also been found, by the 2-D electrophoresis and IEF, that the preparations of 33 ku protein not dialyzed against the low-osmotic medium contain several polypeptides with nearly the same molecular weight but not the same isoelectric point as the 33 ku protein.
基金This work was supported by the State Key Fundamental R & D Project (Grant No. G1999064707) Ministry of Education of China (Department of Foreign Affairs (99)747) German World University Service.
文摘Human cyclophilin 33 (hCyP33), found in 1996, consists of an RNA-binding domain in N-terminus, a cyclophilin domain in C-terminus and a connected part between the two domains. RNA-binding proteins concern functions, such as splicing, modification and transport, after transcription in eukaryotic cells. Cyclophilins (CyPs) possess enzymatic activity, namely peptidyl-proryl cis-trans isomerase (PPIase). They are involved in folding, transport and interaction of proteins. Cyclosporin A (CsA), an immunosuppressant used by organ transplantation, binds to CyPs and suppresses their enzymatic activity. However, up to now it is unknown that which cellular and physiological roles hCyP33, which possesses the above-mentioned both functions, plays. In this paper the binding specificity of hCyP33 to different cellular RNA is investigated by means of ion-exchange chromatography and affinity adsorption. The results show that it binds specifically to poly(A) tailed mRNA, namely poly(A)+RNA.
基金Supported by the National Natural Science Foundation of China(No.81470609 No.81700800+5 种基金 No.81870632 No.81800800)Natural Science Foundation of Shandong Province(No.ZR2013HQ007 No.ZR2017MH008 No.ZR2017BH025)the Youth National Natural Science Foundation of China(No.81500695)
文摘AIM: To investigate the expression of interleukin(IL)-33 in the cornea and human corneal epithelial cells(HCECs) exposed to Aspergillus fumigatus(A. fumigatus), and to determine the function of IL-33/ST2/p38 signaling pathway in the immune response of corneal epithelial cells to A. fumigatus infection.METHODS: The mRNA and protein expression of IL-33 in HCECs and mice corneas were examined by quantitative real-time reverse transcription polymerase chain reaction(q RT-PCR) and Western blot analysis, respectively. IL-33 expression was also detected in cornea samples from healthy donors and patients with fungal keratitis with immunohistochemistry. The cultured HCECs were treated with inactive A. fumigatus hyphae at various concentrations with or without recombinant human IL-33 protein, soluble recombinant ST2 protein, specific ST2 neutralizing antibody, or the mitogen-activated protein kinase(MAPK) p38 inhibitor SB203580 for evaluation of the expression and activation of IL-33/ST2/p38 signaling in the regulation of proinflammatory cytokines. The production levels of IL-6 and IL-1β were determined by qR T-PCR and enzymelinked immunosorbent assay(ELISA). The proliferation of HCECs was determined by a Cell Counting Kit-8(CCK8) assay and cell count.RESULTS: IL-33 expression levels increased in the corneal tissues of patients with fungal keratitis and in mice corneas of experimental A. fumigatus infection,as well as in HCECs with infection of A. fumigatus. A. fumigatus strongly stimulated HCECs-generated proinflammatory cytokine(IL-6 and IL-1β) production at both the mRNA and protein levels. This production of proinflammatory mediators stimulated by A. fumigatus was further stimulated by IL-33 and was prevented by soluble ST2 protein or ST2 neutralizing antibody. Moreover, IL-33 naturally promoted the p38 phosphorylation induced by A. fumigatus, which was suppressed by soluble ST2 protein. The MAPK p38 inhibitor SB203580 also inhibited the A. fumigatus-induced proinflammatory cytokine production. IL-33 administration f
文摘目的探讨脑血管疾病患者血清中白细胞介素33(IL-33)水平与颈动脉粥样硬化斑块稳定性及脑梗死的关系及其作用机制。方法采用ELISA方法检测脑梗死组(n=63)、短暂性脑缺血发作组(n=30)及正常对照组(n=15)血清中IL-33的表达水平;采用Western blotting、荧光定量PCR(qRT-PCR)和ELISA法检测体外培养的人外周血单个核细胞(PBMC)和U937细胞在接受外源性IL-33刺激后,MMP-9和Cox-2的表达量变化。结果脑梗死组IL-33的表达水平低于对照组和短暂性脑缺血发作组(P均<0.01);脑梗死组中不稳定斑块、稳定斑块和无斑块患者血清中IL-33的表达水平逐渐降低(P<0.01);脑梗死组中,与无糖尿病和高血脂症的患者相比,合并糖尿病和高脂血症的患者血清中IL-33水平降低(P均<0.01);外源性IL-33刺激可下调外周血单核细胞和U937细胞中M M P-9和Cox-2的表达和分泌量。结论 IL-33可被作为预测脑梗死及不稳定性粥样硬化斑块的潜在的血清标志物和治疗靶点。
