Foodborne Listeria monocytogenes poses serious threats to public health.Fast and sensitive detection of the pathogen at the point of contamination is thus crucial to halt the spread of bacteria-related diseases.Herein...Foodborne Listeria monocytogenes poses serious threats to public health.Fast and sensitive detection of the pathogen at the point of contamination is thus crucial to halt the spread of bacteria-related diseases.Herein,we report for the first time a fluorescent film sensor to detect the biomarker of L.monocytogenes,3-hydroxy-2-butanone.The sensor demonstrated unprecedented sensing performance for the analyte with a detection limit lower than 0.05 mg/m^(3),response time less than 1 s,full reversibility,and excellent selectivity.Further study showed that the sensor can be used to monitor the growth of L.monocytogenes with much-improved sensitivity.The superior performance of the sensor is ascribed to the specific binding,efficient charge transfer emission,and porous adlayer structure of the specially designed sensing fluorophore-based film.Our work paves the way to develop a portable detector to meet the needs for on-site and real-time detection of foodborne pathogens.展开更多
基金National Natural Science Foundation of China,Grant/Award Numbers:21820102005,22132002111 Project,Grant/Award Number:B14041Fundamental Research Funds for the Central Universities,Grant/Award Number:2019CBLY001。
文摘Foodborne Listeria monocytogenes poses serious threats to public health.Fast and sensitive detection of the pathogen at the point of contamination is thus crucial to halt the spread of bacteria-related diseases.Herein,we report for the first time a fluorescent film sensor to detect the biomarker of L.monocytogenes,3-hydroxy-2-butanone.The sensor demonstrated unprecedented sensing performance for the analyte with a detection limit lower than 0.05 mg/m^(3),response time less than 1 s,full reversibility,and excellent selectivity.Further study showed that the sensor can be used to monitor the growth of L.monocytogenes with much-improved sensitivity.The superior performance of the sensor is ascribed to the specific binding,efficient charge transfer emission,and porous adlayer structure of the specially designed sensing fluorophore-based film.Our work paves the way to develop a portable detector to meet the needs for on-site and real-time detection of foodborne pathogens.
