Background Hospitalized patients often have higher rate of vitamin D deficiency than healthy people. Vitamin D levels below normal are associated with hospital stay, increased incidence of adverse prognosis and increa...Background Hospitalized patients often have higher rate of vitamin D deficiency than healthy people. Vitamin D levels below normal are associated with hospital stay, increased incidence of adverse prognosis and increased mortality of a number of diseases. Whether there is a relationship between vitamin D levels and infection or sepsis in the critically ill is still unclear. This study will explore the relationship between vitamin D levels and risk of infection, assessment for disease severity, and predictor of mortality. Methods To evaluate the value of vitamin D in intensive care unit （ICU） cases to sepsis, severity and prognosis assessment, high performance liquid chromatography and tandem mass spectrometry were used to measure the concentrations of vitamin D in sera of critically ill patients. The serum samples were drawn within the first 24 hours of ICU admission. Results The study included 206 people, 50 healthy controls, 51 ICU control patients and 105 ICU diagnosed with sepsis. Critically ill ICU patients （ICU sepsis and ICU control group） had lower vitamin D concentration than normal people, but septic patients showed no significant reduction of vitamin D concentration when compared with critically ill patients with no positive etiological evidence. For assessment of disease severity, there were very low negative correlations between APACHE II, SAPS II and SOFA scores and vitamin D level. Additionally, patients of different 25-（OH）D levels showed no difference whether in terms of 28-day survival （χ2=1.78, P=0.776） or 90-day survival （χ2=4.12, P=0.369）. Multivariate Logistic regression demonstrated that APECHE II and SAPS II scores were independent risk factors to deaths caused by sepsis. Conclusion Clinically, serum concentration of vitamin D is not an indicator for diagnosis and assessment in critically ill patients （ClinicalTrial.gov identifier NCT01636232）.展开更多
Background： With recognition of the important roles of Vitamin D （VitD） in various physiological processes, increasing attention has been drawn to the status of VitD in early life. However, the VitD status of young...Background： With recognition of the important roles of Vitamin D （VitD） in various physiological processes, increasing attention has been drawn to the status of VitD in early life. However, the VitD status of young children and the related factors in rural areas of Southwestern China remain unclear. This study aimed to explore VitD status and its seasonal variation in 18-month-old children living in rural Southwestern China. The association of VitD with biochemical and anthropometric variables was also investigated. Methods： A total of 177 18-month-old children in a rural area of Yunnan Province, Southwestern China, were enrolled. Serum concentrations of 25-hydroxy Vitamin D （25（OH）D） were measured through high-performance liquid chromatogram-tandem mass spectrometry. Parathyroid hormone （PTH） levels were measured with a chemiluminescence assay. Serum concentrations of calcium, phosphorus, and alkaline phosphatase （ALP） were also measured. Anthropometric data and the outdoor activity time of each participant were collected. Results： The serum 25（OH）D concentration was 26.61 ±7.26 ng/ml; concentrations lower than 30 ng/ml accounted for 70.6% of the participants and concentrations lower than 20 ng/ml accounted for 16.4%. The level of serum 25（OH）D was not significantly different among four seasons （P 〉 0.05）. A positive relationship was found between 25（OH）D concentration and the time of outdoor activities （r = 0.168, P 〈 0.05）. Serum PTH concentration was negatively correlated with 25（OH）D concentration （r - 0.163, P 〈 0.05）. A positive relationship was found between the serum concentrations of 25（OH）D and calcium （r = 0.154, P 〈 0.05）. No significant association was observed between 25（OH）D and ALP, phosphorus, or anthropometric variables. Conclusions： The prevalence of VitD insufficiency is high among young children in the rural Southwestern China regardless of the seasons. VitD supplementation is still essential to maintain VitD suffic展开更多
<strong>Background:</strong> Type 2 diabetes mellitus (T2DM) is a chronic disease that is characterized by <em>β</em>-cell dysfunction and resistance for insulin. Vitamin D is necessary for in...<strong>Background:</strong> Type 2 diabetes mellitus (T2DM) is a chronic disease that is characterized by <em>β</em>-cell dysfunction and resistance for insulin. Vitamin D is necessary for insulin secretion so it is a crucial factor in the development of T2DM. This study was done to investigate the association between serum 25-hydroxy Vitamin D [25(OH)3D], VDR (Vitamin D receptor) and VDBP (Vitamin D binding protein) with type 2 diabetic patients compared to control subjects.<strong> Subjects and Methods:</strong> This study carried out 110 female patients who were previously diagnosed with type 2 diabetes and 110 age, sex and weight matched as controls. All participants were subjected to full history taking, clinical examination and assessment of fasting blood glucose, HbA1c , lipid profile, 25-hydroxy Vitamin D [25(OH)3D], VDR and VDBP. <strong>Results:</strong> Results showed that the level of 25(OH)3D was significantly lower in diabetic group compared to controls and was significantly negatively correlated with glycated hemoglobin, serum total cholesterol and low density lipoprotein cholesterol in type 2 DM. Decreasing Vitamin D level was significantly associated with decreasing VDR. No significant association was found between Vit D and VDBP levels. <strong>Conclusions:</strong> Vitamin D deficiency is frequent in diabetic patients and associated with poor control and outcome. This suggests a role of Vitamin D in the pathogenesis and control of T2DM. Serum VDBP in diabetes may be independent to the level of 25(OH)3D and needs further studies.展开更多
目的检测反复呼吸道感染(RRI)患儿血清25-羟维生素D3[25-(OH)D3]及IgA水平,并行维生素D(VitD)治疗,探讨VitD营养状况与儿童RRI及免疫功能的关系,为临床治疗提供依据。方法选取2010年10月-2011年4月在本院儿科门诊就诊的RRI患儿60例为病...目的检测反复呼吸道感染(RRI)患儿血清25-羟维生素D3[25-(OH)D3]及IgA水平,并行维生素D(VitD)治疗,探讨VitD营养状况与儿童RRI及免疫功能的关系,为临床治疗提供依据。方法选取2010年10月-2011年4月在本院儿科门诊就诊的RRI患儿60例为病例组。年龄1～6(3.91±2.83)岁;男32例,女28例。将病例组随机分为常规治疗组30例和补VitD组30例,均予抗感染及对症治疗,补VitD组另进行补VitD治疗。随机选取同期到门诊体检的健康儿童30例为健康对照组。受检儿童均晨起空腹抽取静脉血3 mL 2份,离心,分离血清。ELISA法检测其血清25-(OH)D3水平,免疫透射比浊法检测其血清IgA水平,进行组间比较。病例组3个月时复查血清25-(OH)D3及IgA水平。病例组儿童随访6个月,观察并记录呼吸道感染的复发次数。结果1.病例组血清25-(OH)D3及IgA水平显著低于健康对照组,2组比较差异均有统计学意义(Pa<0.01)。2.采用不同的方法治疗后补VitD组25-(OH)D3及IgA水平升高,常规治疗组和补VitD组RRI次数比较差异有统计学意义(P<0.01)。结论 RRI患儿血清25-(OH)D3及IgA水平低于健康儿童,低水平25-(OH)D3与RRI有关,提示对RRI患儿应重视VitD的补充。展开更多
目的：观察锌、维生素D、钙联用对婴幼儿佝偻病的疗效及对生化指标的影响。方法：选择80例符合入组标准的婴幼儿佝偻病患儿作为观察对象,按随机数字表法分为观察组和对照组各40例。对照组患儿给予维生素D、钙剂联合治疗,维生素D3肌肉注...目的：观察锌、维生素D、钙联用对婴幼儿佝偻病的疗效及对生化指标的影响。方法：选择80例符合入组标准的婴幼儿佝偻病患儿作为观察对象,按随机数字表法分为观察组和对照组各40例。对照组患儿给予维生素D、钙剂联合治疗,维生素D3肌肉注射30万IU,4周后改为口服维生素AD滴剂1粒/次,qd,维持用药8周,氨基酸螫合钙每次半粒,每日1次,12周为1个疗程;观察组患儿在对照组治疗基础上给予葡萄糖酸锌口服液每天10~20 m L口服,连用12周。观察两组患儿治疗前后症状、体征改善情况,进行X线检查及血液生化检查,包括血锌、血钙、骨碱性磷酸酶（NBAP）、血清25-羟基维生素D3[25-（OH）D3]水平,采用单光子吸收计测定桡骨、尺骨的骨密度,比较两组临床疗效。结果：观察组症状消失和好转率、体征好转率、左手X线片好转率明显高于对照组（χ2分别为12.75、11.67、11.67,P均〈0.01）;观察组和对照组治疗后血钙、25-（OH）D3水平较治疗前明显升高（t分别为6.73、25.66和5.81、20.43,P均〈0.01）,NBAP水平较治疗前明显降低（t分别为8.61、6.24,P均〈0.01）,观察组治疗后血锌水平较治疗前明显升高（t=13.76,P〈0.01）;观察组治疗后血锌、血钙、25-（OH）D3水平明显高于对照组（t分别为6.22、3.63、9.71,P均〈0.05）,NBAP水平明显低于对照组（t=3.74,P〈0.05）;观察组和对照组治疗后桡骨骨密度、尺骨骨密度均明显高于治疗前（t分别为3.76、3.84和3.34、3.26,P均〈0.05）,且观察组治疗后桡骨骨密度、尺骨骨密度明显高于对照组（t分别为3.36、3.62,P均〈0.05）;观察组临床总有效率为92.5%,对照组为72.5%,两组比较差异有统计学意义（χ2=9.589,P〈0.01）。结论：锌、维生素D、钙联用是治疗婴幼儿佝偻病安全、可靠的方法,能相互协同促进儿童佝偻病的康复,值得临床推广应用。展开更多
文摘Background Hospitalized patients often have higher rate of vitamin D deficiency than healthy people. Vitamin D levels below normal are associated with hospital stay, increased incidence of adverse prognosis and increased mortality of a number of diseases. Whether there is a relationship between vitamin D levels and infection or sepsis in the critically ill is still unclear. This study will explore the relationship between vitamin D levels and risk of infection, assessment for disease severity, and predictor of mortality. Methods To evaluate the value of vitamin D in intensive care unit （ICU） cases to sepsis, severity and prognosis assessment, high performance liquid chromatography and tandem mass spectrometry were used to measure the concentrations of vitamin D in sera of critically ill patients. The serum samples were drawn within the first 24 hours of ICU admission. Results The study included 206 people, 50 healthy controls, 51 ICU control patients and 105 ICU diagnosed with sepsis. Critically ill ICU patients （ICU sepsis and ICU control group） had lower vitamin D concentration than normal people, but septic patients showed no significant reduction of vitamin D concentration when compared with critically ill patients with no positive etiological evidence. For assessment of disease severity, there were very low negative correlations between APACHE II, SAPS II and SOFA scores and vitamin D level. Additionally, patients of different 25-（OH）D levels showed no difference whether in terms of 28-day survival （χ2=1.78, P=0.776） or 90-day survival （χ2=4.12, P=0.369）. Multivariate Logistic regression demonstrated that APECHE II and SAPS II scores were independent risk factors to deaths caused by sepsis. Conclusion Clinically, serum concentration of vitamin D is not an indicator for diagnosis and assessment in critically ill patients （ClinicalTrial.gov identifier NCT01636232）.
文摘Background： With recognition of the important roles of Vitamin D （VitD） in various physiological processes, increasing attention has been drawn to the status of VitD in early life. However, the VitD status of young children and the related factors in rural areas of Southwestern China remain unclear. This study aimed to explore VitD status and its seasonal variation in 18-month-old children living in rural Southwestern China. The association of VitD with biochemical and anthropometric variables was also investigated. Methods： A total of 177 18-month-old children in a rural area of Yunnan Province, Southwestern China, were enrolled. Serum concentrations of 25-hydroxy Vitamin D （25（OH）D） were measured through high-performance liquid chromatogram-tandem mass spectrometry. Parathyroid hormone （PTH） levels were measured with a chemiluminescence assay. Serum concentrations of calcium, phosphorus, and alkaline phosphatase （ALP） were also measured. Anthropometric data and the outdoor activity time of each participant were collected. Results： The serum 25（OH）D concentration was 26.61 ±7.26 ng/ml; concentrations lower than 30 ng/ml accounted for 70.6% of the participants and concentrations lower than 20 ng/ml accounted for 16.4%. The level of serum 25（OH）D was not significantly different among four seasons （P 〉 0.05）. A positive relationship was found between 25（OH）D concentration and the time of outdoor activities （r = 0.168, P 〈 0.05）. Serum PTH concentration was negatively correlated with 25（OH）D concentration （r - 0.163, P 〈 0.05）. A positive relationship was found between the serum concentrations of 25（OH）D and calcium （r = 0.154, P 〈 0.05）. No significant association was observed between 25（OH）D and ALP, phosphorus, or anthropometric variables. Conclusions： The prevalence of VitD insufficiency is high among young children in the rural Southwestern China regardless of the seasons. VitD supplementation is still essential to maintain VitD suffic
文摘<strong>Background:</strong> Type 2 diabetes mellitus (T2DM) is a chronic disease that is characterized by <em>β</em>-cell dysfunction and resistance for insulin. Vitamin D is necessary for insulin secretion so it is a crucial factor in the development of T2DM. This study was done to investigate the association between serum 25-hydroxy Vitamin D [25(OH)3D], VDR (Vitamin D receptor) and VDBP (Vitamin D binding protein) with type 2 diabetic patients compared to control subjects.<strong> Subjects and Methods:</strong> This study carried out 110 female patients who were previously diagnosed with type 2 diabetes and 110 age, sex and weight matched as controls. All participants were subjected to full history taking, clinical examination and assessment of fasting blood glucose, HbA1c , lipid profile, 25-hydroxy Vitamin D [25(OH)3D], VDR and VDBP. <strong>Results:</strong> Results showed that the level of 25(OH)3D was significantly lower in diabetic group compared to controls and was significantly negatively correlated with glycated hemoglobin, serum total cholesterol and low density lipoprotein cholesterol in type 2 DM. Decreasing Vitamin D level was significantly associated with decreasing VDR. No significant association was found between Vit D and VDBP levels. <strong>Conclusions:</strong> Vitamin D deficiency is frequent in diabetic patients and associated with poor control and outcome. This suggests a role of Vitamin D in the pathogenesis and control of T2DM. Serum VDBP in diabetes may be independent to the level of 25(OH)3D and needs further studies.
文摘目的检测反复呼吸道感染(RRI)患儿血清25-羟维生素D3[25-(OH)D3]及IgA水平,并行维生素D(VitD)治疗,探讨VitD营养状况与儿童RRI及免疫功能的关系,为临床治疗提供依据。方法选取2010年10月-2011年4月在本院儿科门诊就诊的RRI患儿60例为病例组。年龄1～6(3.91±2.83)岁;男32例,女28例。将病例组随机分为常规治疗组30例和补VitD组30例,均予抗感染及对症治疗,补VitD组另进行补VitD治疗。随机选取同期到门诊体检的健康儿童30例为健康对照组。受检儿童均晨起空腹抽取静脉血3 mL 2份,离心,分离血清。ELISA法检测其血清25-(OH)D3水平,免疫透射比浊法检测其血清IgA水平,进行组间比较。病例组3个月时复查血清25-(OH)D3及IgA水平。病例组儿童随访6个月,观察并记录呼吸道感染的复发次数。结果1.病例组血清25-(OH)D3及IgA水平显著低于健康对照组,2组比较差异均有统计学意义(Pa<0.01)。2.采用不同的方法治疗后补VitD组25-(OH)D3及IgA水平升高,常规治疗组和补VitD组RRI次数比较差异有统计学意义(P<0.01)。结论 RRI患儿血清25-(OH)D3及IgA水平低于健康儿童,低水平25-(OH)D3与RRI有关,提示对RRI患儿应重视VitD的补充。
文摘目的：观察锌、维生素D、钙联用对婴幼儿佝偻病的疗效及对生化指标的影响。方法：选择80例符合入组标准的婴幼儿佝偻病患儿作为观察对象,按随机数字表法分为观察组和对照组各40例。对照组患儿给予维生素D、钙剂联合治疗,维生素D3肌肉注射30万IU,4周后改为口服维生素AD滴剂1粒/次,qd,维持用药8周,氨基酸螫合钙每次半粒,每日1次,12周为1个疗程;观察组患儿在对照组治疗基础上给予葡萄糖酸锌口服液每天10~20 m L口服,连用12周。观察两组患儿治疗前后症状、体征改善情况,进行X线检查及血液生化检查,包括血锌、血钙、骨碱性磷酸酶（NBAP）、血清25-羟基维生素D3[25-（OH）D3]水平,采用单光子吸收计测定桡骨、尺骨的骨密度,比较两组临床疗效。结果：观察组症状消失和好转率、体征好转率、左手X线片好转率明显高于对照组（χ2分别为12.75、11.67、11.67,P均〈0.01）;观察组和对照组治疗后血钙、25-（OH）D3水平较治疗前明显升高（t分别为6.73、25.66和5.81、20.43,P均〈0.01）,NBAP水平较治疗前明显降低（t分别为8.61、6.24,P均〈0.01）,观察组治疗后血锌水平较治疗前明显升高（t=13.76,P〈0.01）;观察组治疗后血锌、血钙、25-（OH）D3水平明显高于对照组（t分别为6.22、3.63、9.71,P均〈0.05）,NBAP水平明显低于对照组（t=3.74,P〈0.05）;观察组和对照组治疗后桡骨骨密度、尺骨骨密度均明显高于治疗前（t分别为3.76、3.84和3.34、3.26,P均〈0.05）,且观察组治疗后桡骨骨密度、尺骨骨密度明显高于对照组（t分别为3.36、3.62,P均〈0.05）;观察组临床总有效率为92.5%,对照组为72.5%,两组比较差异有统计学意义（χ2=9.589,P〈0.01）。结论：锌、维生素D、钙联用是治疗婴幼儿佝偻病安全、可靠的方法,能相互协同促进儿童佝偻病的康复,值得临床推广应用。
