The neutron capture cross section of 197 Au was measured using the time-of-flight(TOF)technique at the Back-n facility of the China Spallation Neutron Source(CSNS)in the 1 eV to 100 keV range.Prompt c-rays originating...The neutron capture cross section of 197 Au was measured using the time-of-flight(TOF)technique at the Back-n facility of the China Spallation Neutron Source(CSNS)in the 1 eV to 100 keV range.Prompt c-rays originating from neutron-induced capture events were detected by four C_(6)D_(6) liquid scintillator detectors.Pulse height weighting technology(PHWT)was used to analyze the data.The results are in good agreement with ENDF/B-VIII.0,CENDL-3.1,and other evaluated libraries in the resonance region,and in agreement with both n TOF and GELINA experimental data in the 5–100 keV range.Finally,the resonance peaks in the energy range from 1eV to 1 keV were fitted by the SAMMY R-matrix code.展开更多
We have previously shown that the diphtheria toxin variant CRM 197 （cross-reacting material 197） can be overexpressed in Escherichia coli at high levels, yielding insoluble aggregates, which were solubilized using u...We have previously shown that the diphtheria toxin variant CRM 197 （cross-reacting material 197） can be overexpressed in Escherichia coli at high levels, yielding insoluble aggregates, which were solubilized using urea. This study reports a comparison of three matrices suitable for the purification and refolding of recombinant CRM197 by metal-chelating affinity chromatography, Moreover, we show that refolded CRM197 features enzymatic activity.展开更多
基金supported by the National Natural Science Foundation of China(Nos.11875311,11905274,11705156,and 11605097)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB34030000)。
文摘The neutron capture cross section of 197 Au was measured using the time-of-flight(TOF)technique at the Back-n facility of the China Spallation Neutron Source(CSNS)in the 1 eV to 100 keV range.Prompt c-rays originating from neutron-induced capture events were detected by four C_(6)D_(6) liquid scintillator detectors.Pulse height weighting technology(PHWT)was used to analyze the data.The results are in good agreement with ENDF/B-VIII.0,CENDL-3.1,and other evaluated libraries in the resonance region,and in agreement with both n TOF and GELINA experimental data in the 5–100 keV range.Finally,the resonance peaks in the energy range from 1eV to 1 keV were fitted by the SAMMY R-matrix code.
文摘We have previously shown that the diphtheria toxin variant CRM 197 （cross-reacting material 197） can be overexpressed in Escherichia coli at high levels, yielding insoluble aggregates, which were solubilized using urea. This study reports a comparison of three matrices suitable for the purification and refolding of recombinant CRM197 by metal-chelating affinity chromatography, Moreover, we show that refolded CRM197 features enzymatic activity.
