目的:^(64)Cu有半衰期较长、化学反应温和的优势,本研究合成新型乏氧分子影像学探针^(64)Cu-ATSM,并与18F-氟硝基咪唑(^(18)F-fluoromisonidazole,18F-FMISO)进行初步比较。方法:使用SIEMENS Eclipse HP 11 Me V小型医用回旋加速器系统,...目的:^(64)Cu有半衰期较长、化学反应温和的优势,本研究合成新型乏氧分子影像学探针^(64)Cu-ATSM,并与18F-氟硝基咪唑(^(18)F-fluoromisonidazole,18F-FMISO)进行初步比较。方法:使用SIEMENS Eclipse HP 11 Me V小型医用回旋加速器系统,Comecer ALCEO EDL、PRF、TADDEO放射性模块和定制铂金靶盘进行64Ni电镀、^(64)Cu纯化和^(64)Cu-ATSM合成。用伽马能谱仪和高效液相色谱(high performance liquid chromatography,HPLC)进行放射核纯度、放化纯度和稳定性检测。在荷人乳腺癌MDB-MA-231模型中进行小动物PET/CT显像并与自制的^(18)F-FMISO进行比较。结果:实现了^(64)Cu-ATSM的自动化生产,产率60%,放射性核纯度>99%,放化纯度>95%,标记物稳定性>6 h。与^(18)F-FMISO一样能反映肿瘤乏氧情况,且注射1 h后T/M值即能达5.28。结论:成功用11 Me V SIEMENS回旋加速器和Comecer ALCEO模块合成了^(64)Cu-ATSM,荷人乳腺癌小动物PET/CT乏氧显像靶/本比高。展开更多
Aim:The purpose of this study was to locate the levels of hypoxia in glioblastoma PET images measured with 18F-fluoromisonidazole in human subjects.It is recognized that tumors with hypoxia are resistant to treatment ...Aim:The purpose of this study was to locate the levels of hypoxia in glioblastoma PET images measured with 18F-fluoromisonidazole in human subjects.It is recognized that tumors with hypoxia are resistant to treatment by radiotherapy and chemotherapy.Methods:The images were acquired in dynamic mode for 15 min or 30 min and in static mode for two single scans at 2 h and 3 h to allow the accumulation of the radiotracer in the tumor.The images were analyzed at the voxel basis with compartmental analysis(CA)and with the usual tumor-to-blood uptake ratio(TBR).Kmeans algorithm was applied to cluster the levels of hypoxia in the images.Results:TBR at a threshold of 1.2 at imaging times of 15 min,2 h and 3 h produced images with different clusters.Also,the comparison of TBR with the distribution volume obtained with CA had a similarity index of 0.61±0.05.Conclusion:We found some differences in defining the hypoxic volume within a tumor using TBR.The compartmental analysis allowed discrimination of the tumor hypoxic sub-volumes which can be useful for a better treatment with radiotherapy.展开更多
文摘目的:^(64)Cu有半衰期较长、化学反应温和的优势,本研究合成新型乏氧分子影像学探针^(64)Cu-ATSM,并与18F-氟硝基咪唑(^(18)F-fluoromisonidazole,18F-FMISO)进行初步比较。方法:使用SIEMENS Eclipse HP 11 Me V小型医用回旋加速器系统,Comecer ALCEO EDL、PRF、TADDEO放射性模块和定制铂金靶盘进行64Ni电镀、^(64)Cu纯化和^(64)Cu-ATSM合成。用伽马能谱仪和高效液相色谱(high performance liquid chromatography,HPLC)进行放射核纯度、放化纯度和稳定性检测。在荷人乳腺癌MDB-MA-231模型中进行小动物PET/CT显像并与自制的^(18)F-FMISO进行比较。结果:实现了^(64)Cu-ATSM的自动化生产,产率60%,放射性核纯度>99%,放化纯度>95%,标记物稳定性>6 h。与^(18)F-FMISO一样能反映肿瘤乏氧情况,且注射1 h后T/M值即能达5.28。结论:成功用11 Me V SIEMENS回旋加速器和Comecer ALCEO模块合成了^(64)Cu-ATSM,荷人乳腺癌小动物PET/CT乏氧显像靶/本比高。
文摘Aim:The purpose of this study was to locate the levels of hypoxia in glioblastoma PET images measured with 18F-fluoromisonidazole in human subjects.It is recognized that tumors with hypoxia are resistant to treatment by radiotherapy and chemotherapy.Methods:The images were acquired in dynamic mode for 15 min or 30 min and in static mode for two single scans at 2 h and 3 h to allow the accumulation of the radiotracer in the tumor.The images were analyzed at the voxel basis with compartmental analysis(CA)and with the usual tumor-to-blood uptake ratio(TBR).Kmeans algorithm was applied to cluster the levels of hypoxia in the images.Results:TBR at a threshold of 1.2 at imaging times of 15 min,2 h and 3 h produced images with different clusters.Also,the comparison of TBR with the distribution volume obtained with CA had a similarity index of 0.61±0.05.Conclusion:We found some differences in defining the hypoxic volume within a tumor using TBR.The compartmental analysis allowed discrimination of the tumor hypoxic sub-volumes which can be useful for a better treatment with radiotherapy.
