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太湖地区不同轮作模式下的稻田氮素平衡研究 被引量:12
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作者 胡安永 刘勤 +1 位作者 孙星 张亚楠 《中国生态农业学报》 CAS CSCD 北大核心 2014年第5期509-515,共7页
采用田间微区15N 示踪,研究了太湖地区稻田不同轮作模式(紫云英-水稻轮作、休闲-水稻轮作、小麦-水稻轮作)和施氮水平(0、120 kg·hm-2、240 kg·hm-2、300 kg·hm-2)下水稻对氮肥的吸收利用效率及土壤氮素残留特征。... 采用田间微区15N 示踪,研究了太湖地区稻田不同轮作模式(紫云英-水稻轮作、休闲-水稻轮作、小麦-水稻轮作)和施氮水平(0、120 kg·hm-2、240 kg·hm-2、300 kg·hm-2)下水稻对氮肥的吸收利用效率及土壤氮素残留特征。结果表明,水稻吸收的氮素来自肥料的比例为20.9%-49.6%,休闲-水稻轮作模式下水稻产量的获得更加依赖无机氮肥的大量投入。当季水稻对肥料氮的利用率为25.0%-41.5%,肥料氮的土壤残留率为13.4%-24.6%,其中90%以上的土壤残留肥料氮集中在0-20 cm土层,在土壤剖面中的残留率随土层深度增加而迅速降低,30-40 cm土层的肥料残留量仅占氮肥施用量的0.2%-0.7%。紫云英-水稻轮作和休闲-水稻轮作模式下氮肥利用率和土壤残留率均在施氮240 kg·hm-2时达到最大值,其氮肥利用率显著高于小麦-水稻轮作55.6%和66.0%。稻季施氮240 kg·hm-2时,小麦-水稻轮作模式下的氮肥利用率、土壤残留率以及总回收率显著最低,损失率显著最大;紫云英-水稻轮作模式下的氮肥损失率最小,分别小于休闲-水稻轮作和小麦-水稻轮作13.9%、39.2%。不同轮作模式下,水稻籽粒产量随施氮量的增加而增加,稻季施氮240 kg·hm-2时,紫云英-水稻轮作下水稻籽粒产量显著高于休闲-水稻轮作和小麦-水稻轮作,小麦-水稻轮作籽粒产量虽略高于休闲-水稻轮作,但没有达到显著水平。本研究认为,选择紫云英还田配施氮肥240 kg·hm-2,既可以保证水稻氮肥利用率而获得高产,又能减少氮肥损失而带来的环境风险,是一种值得在当地大力推广的耕作制度。 展开更多
关键词 15n标记尿素 轮作模式 施氮量 氮肥利用率 肥料残留 水稻
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施氮时期对‘黑比诺’葡萄氮素吸收、分配及利用的影响 被引量:4
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作者 吴茂东 马宗桓 +4 位作者 李彦彪 马维峰 赵津 李强 陈佰鸿 《甘肃农业大学学报》 CAS CSCD 北大核心 2020年第5期113-120,共8页
【目的】施氮时期对酿酒葡萄氮素吸收、分配及利用的影响,为河西走廊葡萄园合理施肥提供理论依据.【方法】以4年生酿酒葡萄‘黑比诺’(Vitis vinifera L.cv.Pinot Noir)为试材,应用15 N示踪技术分别在萌芽期(T 1)、新梢旺长期(T 2)、果... 【目的】施氮时期对酿酒葡萄氮素吸收、分配及利用的影响,为河西走廊葡萄园合理施肥提供理论依据.【方法】以4年生酿酒葡萄‘黑比诺’(Vitis vinifera L.cv.Pinot Noir)为试材,应用15 N示踪技术分别在萌芽期(T 1)、新梢旺长期(T 2)、果实膨大期(T 3)和果实转色期(T 4)一次性施入速效氮肥,每株均匀沟施15 N-尿素10 g、普通尿素90 g.果实采收期将各处理植株样品整株解析,进行氮的分析测定.【结果】T 2施肥处理植株在果实成熟时氮素累积量最高,达32.0 g/株,各施肥处理植株,不同器官间以叶片氮素累积量最高.植株新梢、叶片和果实的15 N吸收征调能力在4个施肥处理间呈T 1>T 3>T 2>T 4,T 1、T 2和T 4施肥处理,植株叶的15 N吸收征调能力最低,N dff值分别为7.73%、3.52%、1.29%.不同施肥处理,植株各器官15 N分配率存在差异,其中T 2施肥处理中15 N分配率以营养器官最高、生殖器官最低,其值分别为72.3%和6.4%.此外,T 2施肥处理植株在果实成熟时15 N利用率最高,达17.77%.【结论】综合分析认为新梢旺长期是河西走廊酿酒葡萄园氮肥施用的最关键时期. 展开更多
关键词 施氮时期 黑比诺 15 n-尿素 吸收 分配 利用
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太湖地区水稻季氮肥的作物回收和损失研究 被引量:31
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作者 田玉华 尹斌 +1 位作者 贺发云 朱兆良 《植物营养与肥料学报》 CAS CSCD 北大核心 2009年第1期55-61,共7页
在太湖地区水稻土上,采用田间微区^(15)N示踪试验研究了不同氮磷肥配合下水稻季氮肥去向以及残留肥料氮在麦季的吸收利用。结果表明,水稻当季作物对肥料氮的回收率为29%~39%,土壤残留肥料氮的后效很低,后季冬小麦仅利用土壤残留肥料氮... 在太湖地区水稻土上,采用田间微区^(15)N示踪试验研究了不同氮磷肥配合下水稻季氮肥去向以及残留肥料氮在麦季的吸收利用。结果表明,水稻当季作物对肥料氮的回收率为29%~39%,土壤残留肥料氮的后效很低,后季冬小麦仅利用土壤残留肥料氮的2.4%~5.2%。经过连续两个稻麦轮作,0—60 cm土壤中残留肥料氮占施氮量的11%~13%,绝大多数在0—20 cm表层土中。水稻季施用的肥料氮向耕层以下移动很少,20—60 cm土层中累积肥料氮仅占施氮量的0.6%~1.1%,主要发生在小麦季及水稻泡田时期,肥料氮损失占施氮量的47%~54%,氨挥发和硝化反硝化气态损失是主要途径。高氮和高磷处理没有增加作物产量和氮肥利用率,过量施氮或施磷无益于作物增产和氮肥吸收利用。 展开更多
关键词 ^15n标记尿素 稻麦轮作 残留肥料氮
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关于不同籼稻品种和施肥时期稻株对^(15)N的吸收及其分配的研究 被引量:35
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作者 王维金 《作物学报》 CAS CSCD 北大核心 1994年第4期476-480,共5页
氮肥吸收率随不同水稻品种产量水平的提高而提高,稻株对氮肥的吸收率在分蘖期各品种之间差异不显著,而在灌浆成熟期表现其显著差异,即矮秆水稻品种和杂交稻对氮肥的吸收利用率高于高秆水稻品种。杂交稻汕优63,稻株对穗肥^(15)N的吸收利... 氮肥吸收率随不同水稻品种产量水平的提高而提高,稻株对氮肥的吸收率在分蘖期各品种之间差异不显著,而在灌浆成熟期表现其显著差异,即矮秆水稻品种和杂交稻对氮肥的吸收利用率高于高秆水稻品种。杂交稻汕优63,稻株对穗肥^(15)N的吸收利用率明显高于基肥^(15)N和分蘖肥^(15)N。杂交稻汕优63在抽穗后20天,有50%或50%以上被吸收的^(15)N转运至穗部·但穗部^(15)N的含量则因施肥时期而不同,即穗肥明显高于基肥和分蘖肥。 展开更多
关键词 籼稻 品种 施肥 氮肥吸收率
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Fate of ^(15)N-Labeled Urea Under a Winter Wheat-Summer Maize Rotation on the North China Plain 被引量:44
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作者 JU Xiao-Tang LIU Xue-Jun +1 位作者 PAN Jia-Rong ZHANG Fu-Suo 《Pedosphere》 SCIE CAS CSCD 2007年第1期52-61,共10页
A field experiment was conducted to investigate the fate of ^15N-labeled urea and its residual effect under the winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) rotation system on the North China... A field experiment was conducted to investigate the fate of ^15N-labeled urea and its residual effect under the winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) rotation system on the North China Plain. Compared to a conventional application rate of 360 kg N ha^-1 (N360), a reduced rate of 120 kg N ha^-1 (N120) led to a significant increase (P 〈 0.05) in wheat yield and no significant differences were found for maize. However, in the 0-100 cm soil profile at harvest, compared with N360, N120 led to significant decreases (P 〈 0.05) of percent residual N and percent unaccounted-for N, which possibly reflected losses from the managed system. Of the residual fertilizer N in the soil profile, 25.6%-44.7% and 20.7%-38.2% for N120 and N360, respectively, were in the organic N pool, whereas 0.3%-3.0% and 11.2%-24.4%, correspondingly, were in the nitrate pool, indicating a higher potential for leaching loss associated with application at the conventional rate. Recovery of residual N in the soil profile by succeeding crops was less than 7.5% of the applied N. For N120, total soil N balance was negative; however, there was still considerable mineral N (NH4^+-N and NO3^--N) in the soil profile after harvest. Therefore, N120 could be considered ngronomically acceptable in the short run, but for long-term sustainability, the N rate should be recommended based on a soil mineral N test and a plant tissue nitrate test to maintain the soil fertility. 展开更多
关键词 ^15n-labeled urea nitrogen recovery nitrogen residual effect north China Plain winter wheat-summer maize rotation
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^(15)N尿素在兔体内消化吸收及利用机理研究 Ⅰ.兔对^(15)N尿素利用的氮平衡状况及形态分配 被引量:11
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作者 高家骅 周维仁 +3 位作者 章云 于亚君 陈明 黄玲 《江苏农业学报》 CSCD 北大核心 1998年第4期223-227,共5页
应用稳定同位素15N尿素示踪技术,经兔消化代谢试验,研究尿素氮平衡状况,测定其消化率和利用系数,并对在兔体内形成蛋白质沉积及其它含氮化合物形态与分配进行了讨论,肯定了尿素氮作为补充兔日粮中粗蛋白营养不足的价值。研究发... 应用稳定同位素15N尿素示踪技术,经兔消化代谢试验,研究尿素氮平衡状况,测定其消化率和利用系数,并对在兔体内形成蛋白质沉积及其它含氮化合物形态与分配进行了讨论,肯定了尿素氮作为补充兔日粮中粗蛋白营养不足的价值。研究发现,15N尿素在胃和小肠内容物中,具有与盲肠中相似的由微生物转化合成极易被兔消化利用的游离氨基酸15N和蛋白质15N等化合物的功能,从而促进了兔对日粮尿素的消化吸收和利用。 展开更多
关键词 饲料添加剂 尿素 氮平衡 消化 吸收
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分次追施N肥对苹果砧木—平邑甜茶吸收^(15)N-尿素以及叶片衰老的影响 被引量:7
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作者 丁宁 姜远茂 +2 位作者 魏绍冲 陈倩 葛顺峰 《植物生态学报》 CAS CSCD 北大核心 2012年第12期1286-1292,共7页
以盆栽平邑甜茶(Malus hupenhensis)为实验材料,研究等氮(N)量分次追施N肥(一次、二次和三次)对平邑甜茶叶片衰老及15N-尿素吸收、利用的影响。采用15N示踪技术,研究不同施肥处理下植株的生长、酶活性和15N吸收利用等参数。研究结果表明... 以盆栽平邑甜茶(Malus hupenhensis)为实验材料,研究等氮(N)量分次追施N肥(一次、二次和三次)对平邑甜茶叶片衰老及15N-尿素吸收、利用的影响。采用15N示踪技术,研究不同施肥处理下植株的生长、酶活性和15N吸收利用等参数。研究结果表明:植株的株高、茎粗、叶面积和叶绿素含量(SPAD)在生长前期均以一次性追肥处理最高,三次追肥处理最低,且与一次追肥处理差异显著;在生长中期均以二次追肥处理最高,一次追肥处理最低;在生长后期均以三次追肥处理最高,一次追肥处理最低,且与三次追肥处理差异显著;叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性在生长前期均以一次追肥处理最高,三次追肥处理最低,且与一次追肥处理差异显著;在生长中期均以二次追肥处理最高,一次追肥处理最低;在生长后期均以三次追肥处理最高,一次追肥处理最低,且与三次追肥处理差异显著;生长后期植株各器官从肥料中吸收分配到的15N量对该器官全N量的贡献率差异显著,三次追肥处理显著高于一次和二次追肥处理;生长后期三次追肥处理植株的总N量、吸收的15N量及15N肥料利用率均为最大。三次追肥处理能提高叶片全N量,延缓生长后期叶片衰老,提高N肥利用率。 展开更多
关键词 吸收 分次追肥 叶片衰老 平邑甜茶 15n标记的尿素 利用
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Effect of Transplanting Density on Rice Yield,Nitrogen Uptake and ^(15)N-fertilizer Fate 被引量:5
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作者 樊红柱 张鸿 +2 位作者 冯文强 张冀 王昌桃 《Agricultural Science & Technology》 CAS 2012年第5期1037-1039,1054,共4页
[Objective] The aim of this study was investigated the rice yield, nitrogen uptake and ^15-fertilizer fate at different transplanting density to provide scientific ba- sis for improving the yield of rice and applying ... [Objective] The aim of this study was investigated the rice yield, nitrogen uptake and ^15-fertilizer fate at different transplanting density to provide scientific ba- sis for improving the yield of rice and applying reasonably fertilizer. [Method] A field experiment was carried out to study the effect of different transplanting density on rice yield, nitrogen (N) absorption, sources of N uptake by rice and the N balance in the plant-soil systems by using ^15-labelled urea. [Result] There were no significant differences in rice yields and total N uptakes by rice between treatments 30 cm × 30 cm and 40 cm × 40 cm, but the yield of rice and total N absorption in the two treatments were remarkably higher than those in 50 cm × 50 cm treatment. The amounts of total N uptake by rice were in the range of 112.3-162.7 kg/hm2 in the three transplanting densities. The result showed that about 1/3 of the total N uptake by rice was supplied by application fertilizer and the other 2/3 was obtained from the soil N pool. The ^15N-labelled urea absorbed by rice, residual in soil and lost accounted for 16.3%-26.1%, 17.0%-20.9% and 53.0%-66.7% of the total fertilizer, respectively. A great deal of ^15N-labelled urea was lost during the rice growing season. [Conclusion] Considering the rice yield and environmental protection, the transplanting density of 30 cm×30 cm was recommended in the hilly area of Sichuan basin in the southwest China. 展开更多
关键词 Rice yield nitrogen uptake and balance ^15n-labeled urea ^15n fate Transplanting density
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Hole fertilization in the root zone facilitates maize yield and nitrogen utilization by mitigating potential N loss and improving mineral N accumulation 被引量:2
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作者 SHI Wen-xuan ZHANG Qian +3 位作者 LI Lan-tao TAN Jin-fang XIE Ruo-han WANG Yi-lun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第4期1184-1198,共15页
Reducing environmental impacts and improving N utilization are critical to ensuring food security in China.Although root-zone fertilization has been considered an effective strategy to improve nitrogen use efficiency ... Reducing environmental impacts and improving N utilization are critical to ensuring food security in China.Although root-zone fertilization has been considered an effective strategy to improve nitrogen use efficiency (NUE),the effect of controlled-release urea (CRU) applied in conjunction with normal urea in this mode is unclear.Therefore,a 3-year field experiment was conducted using a no-N-added as a control and two fertilization modes (FF,furrow fertilization by manual trenching,i.e.,farmer fertilizer practice;HF:root-zone hole fertilization by point broadcast manually) at 210 kg N ha^(–1) (controlled-release:normal fertilizer=5:5),along with a 1-year in-situ microplot experiment.Maize yield,NUE and N loss were investigated under different fertilization modes.The results showed that compared with FF,HF improved the average yield and N recovery efficiency by 8.5 and 22.3%over three years,respectively.HF had a greater potential for application than FF treatment,which led to increases in dry matter accumulation,total N uptake,SPAD value and LAI.In addition,HF remarkably enhanced the accumulation of ^(15)N derived from fertilizer by 17.2%compared with FF,which in turn reduced the potential loss of^(15)N by 43.8%.HF increased the accumulation of N in the tillage layer of soils at harvest for potential use in the subsequent season relative to FF.Hence,HF could match the N requirement of summer maize,sustain yield,improve NUE and reduce environmental N loss simultaneously.Overall,root-zone hole fertilization with blended CRU and normal urea can represent an effective and promising practice to achieve environmental integrity and food security on the North China Plain,which deserves further application and investigation. 展开更多
关键词 maize yield hole fertilization nUE ^(15)n-labeled blended urea ^(15)n loss
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基于酶催化反应测定^(15)N标记尿素同位素丰度
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作者 赵诚 解龙 徐巾岚 《肥料与健康》 CAS 2022年第4期57-62,共6页
基于酶催化反应建立^(15)N标记尿素同位素丰度的测定方法。10 mg ^(15)N标记尿素先在50 mg脲酶溶液中催化生成^(15)N标记碳酸铵,然后在碱性、65℃下分解产生^(15)N标记氨气并用酸吸收后生成^(15)N标记铵盐,^(15)N标记铵盐与次溴酸钠反... 基于酶催化反应建立^(15)N标记尿素同位素丰度的测定方法。10 mg ^(15)N标记尿素先在50 mg脲酶溶液中催化生成^(15)N标记碳酸铵,然后在碱性、65℃下分解产生^(15)N标记氨气并用酸吸收后生成^(15)N标记铵盐,^(15)N标记铵盐与次溴酸钠反应生成^(15)N标记氮气,再将生成的^(15)N标记气体引入MAT-271型气体同位素质谱仪中检测相对原子质量为28、29和30的离子流强度,根据不同丰度范围计算^(15)N同位素丰度。结果表明:针对低、中、高不同^(15)N同位素丰度的尿素样品,采用脲酶催化法测定的^(15)N丰度与微量高温燃烧法的偏差均小于0.2%,相对标准偏差均小于0.3%,适用的^(15)N同位素丰度范围为0.365%~99.2%。脲酶催化法准确度较高,精密度良好,适用于^(15)N标记尿素同位素丰度的检测。 展开更多
关键词 酶催化反应 脲酶催化法 ^(15)n标记尿素 ^(15)n同位素丰度
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Different fertilizer types affected nitrogen and carbon cycling in eroded and colluvial soils of Southern Ecuador
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作者 Etienne Bahr Ute Hamer +1 位作者 Diego Chamba Zaragocin Franz Makeschin 《Agricultural Sciences》 2013年第12期19-32,共14页
A tropical smallholder cash crop farming system in a semi-arid Inter-Andean valley of Ecuador was investigated. Intensive irrigated agriculture with up to 400 kg of urea-N fertilization ha-1·a-1 prevails in collu... A tropical smallholder cash crop farming system in a semi-arid Inter-Andean valley of Ecuador was investigated. Intensive irrigated agriculture with up to 400 kg of urea-N fertilization ha-1·a-1 prevails in colluvial sites whereas urea application in the steep eroded sites is lower. Farmers did recently introduce manure to partly replace urea fertilization. Knowledge about the effects of mineral and organic fertilizers on nitrogen and carbon cycling needs to be improved to maintain sustainable agriculture in the research area. Therefore, a laboratory incubation experiment was conducted to investigate effects of urea and guinea pig manure on microbial activity of col-luvial foot slope and eroded upper slope soils. Soil samples were treated with 200 kg·N·ha-1 either applied as urea, guinea pig manure or a combination of both and compared to a control. Urea fertilizer was labeled with 15N to trace its fate. Microbial biomass and basal respiration were determined in different time intervals. Urea fertilization induced an acceleration of soil organic matter (SOM) mineralization (positive priming effect) in the first seven days of incubation. The combined fertilization served as a positive alternative to the present urea management since it increased the amount of microbial biomass and provided mineral nitrogen for immediate plant uptake. Moreover, low recovery rates suggested an immobilization of fertilized nitrogen into the microbial biomass inducing an efficient and sustained nutrient release throughout the entire growing season. SOM stocks in colluvial soils were 40% below those of eroded soils which was partly due to the positive priming effect after urea fertilization. Microbial activity and efficiency were not reduced by soil erosion. 展开更多
关键词 FERTILIZATIOn 15n-labeled urea GUInEA PIG MAnURE Erosion PRIMInG Effect Soil Organic Matter
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不同氮水平下橡胶树氮素贮藏及翌年分配利用特性 被引量:12
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作者 张永发 吴小平 +5 位作者 王文斌 陈艳彬 罗雪华 薛欣欣 王大鹏 赵春梅 《热带作物学报》 CSCD 北大核心 2019年第12期2313-2320,共8页
以2年生幼龄橡胶树为试材,采用落叶期换土移栽法,利用^15N同位素示踪技术,研究了少量施氮(N28)、适量施氮(N56)和过量施氮(N84)3个氮素水平下幼树的生长差异及氮吸收、利用和分配特性。结果表明:适量施氮肥利于树体生长。以N28处理为对... 以2年生幼龄橡胶树为试材,采用落叶期换土移栽法,利用^15N同位素示踪技术,研究了少量施氮(N28)、适量施氮(N56)和过量施氮(N84)3个氮素水平下幼树的生长差异及氮吸收、利用和分配特性。结果表明:适量施氮肥利于树体生长。以N28处理为对照,N56和N84处理均通过促进根系生长进而促进地上部生长,且N56处理对地上部生长的促进作用较N84更为显著。N28、N56和N84处理橡胶树当年氮肥利用率分别为47.55%、46.83%、39.09%,在第2年春季第一蓬叶稳定期后,各处理氮肥利用率分别为44.49%、43.79%、38.17%。橡胶树氮素的主要贮藏部位为主干和根系,其^15N分配率为59.58%左右,主干木质部的^15N分配率最高,N28、N56和N84处理分别为24.65%、28.69%和25.50%;3个处理地上部枝干中的^15N分配率为76.85%(N28)、78.24%(N56)和75.51%(N84)。经过春季的重新再利用,第1年吸收贮藏的氮素由枝干和根系向新生器官(新梢木质部、新梢皮部、叶片及叶柄)大量运转,满足其生长发育的需要;N28、N56和N84处理新生器官中的Ndff%较高,分别为9.60%~11.31%、18.39%~21.43%和31.67%~34.04%,而主干木质部中的Ndff%较低,分别为3.86%、7.90%和13.77%。贮藏氮在橡胶树春季器官的生长发育中起到重要作用,3个处理新生器官中的^15N分配率为50.60%(N28)、53.98%(N56)和53.28%(N84)。适量施氮水平下^15N在地上部枝干中的贮藏比例较高,翌年新生器官中的分配率也高,有利于橡胶树氮素的季节性循环利用及生长发育的需要。 展开更多
关键词 幼龄橡胶树 ^15n-尿素 贮藏 分配利用
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