This paper for the first time reveals high-resolution core records of Zabuye Salt Lake in the interior of the Qinghai-Tibet Plateau. According to 1346 samples taken continuously, relatively accurate 14^C, U-series dis...This paper for the first time reveals high-resolution core records of Zabuye Salt Lake in the interior of the Qinghai-Tibet Plateau. According to 1346 samples taken continuously, relatively accurate 14^C, U-series disequilibrium and ESR ages have been obtained, thus revealing that the lake core ages from 0 to 83.63 m of hole SZK02 are -800 to over 128 ka. In the paper, the lake core sedimentary characteristics （including the lithologies and mineral assemblages） are analyzed in detail and correlated with ostracod assemblages I to XX and sporopollen zones A to I, and on the basis of an integrated analysis of the δ^18O values of authigenic calcium-magnesium carbonate and environmental proxies of minerals, sporopollen and microfossils in the lake core, a correlation has been made of oxygen isotope change between this lake core and the Greenland GISP2 and GRIP and Guliya ice cores, and the climate of Zabuye Salt Lake since 128 ka BP is divided into the last interglacial stage （including substages e, d, c, b and a） of oxygen isotope stage （OIS） 5, early glacial stadial of the last glacial stage of OIS 4, interglacial stadial of the last glacial stage of OIS 3, late glacial stadial of the last glacial stage or Last Glacial Maximum of OIS 2 and postglacial state of OIS 1; in addition, 6 Heinrich （H6-H1） events, Younger Dryas event and 8.2 ka BP cold event have been recognized.展开更多
Background：XB 130 is a recently discovered adaptor protein that is highly expressed in many malignant tumors,but few studies have investigated its role in hepatocellular carcinoma （HCC）.Therefore,this study explore...Background：XB 130 is a recently discovered adaptor protein that is highly expressed in many malignant tumors,but few studies have investigated its role in hepatocellular carcinoma （HCC）.Therefore,this study explored the relationship between this protein and liver cancer and investigated its molecular mechanism of action.Methods：The expression of XB 130 between HCC tissues and adjacent nontumor tissues was compared by real-time polymerase chain reaction,immunochemistry,and Western blotting.XB130 silencing was performed using small hairpin RNA.The effect of silencing XB130 was examined using Cell Counting Kit-8,colony assay,wound healing assay,and cell cycle analysis.Results：We found that XB 130 was highly expressed in HCC tissues （cancer tissues vs.adjacent tissues：0.23 ± 0.02 vs.0.17 ± 0.02,P 〈 0.05） and liver cancer cell lines,particularly MHCC97H and HepG2 （MHCC97H and HepG2 vs.normal liver cell line LO-2：2.35 ± 0.26 and 2.04 ± 0.04 vs.1.00 ± 0.04,respectively,all P 〈 0.05）.The Cell Counting Kit-8 assay,colony formation assay,and xenograft model in nude mice showed that silencing XB130 inhibited cell proliferative ability both in vivo and in vitro,with flow cytometry demonstrating that the cells were arrested in the G0/G1 phase in HepG2 （HepG2 XB130-silenced group [shA] vs.HepG2 scramble group [NA]：74.32 ± 5.86% vs.60.21 ± 3.07%,P 〈 0.05） and that the number of G2/M phase cells was decreased （HepG2 shA vs.HepG2 NA：8.06 ± 2.41% vs.18.36 ± 4.42%,P 〈 0.05）.Furthermore,the cell invasion and migration abilities were impaired,and the levels of the epithelial-mesenchymal transition-related indicators vimentin and N-cadherin were decreased,although the level of E-cadherin was increased after silencingXB130.Western blotting showed that the levels of phosphorylated phosphoinositide 3-kinase （PI3K） and phospho-protein kinase B （p-Akt） also increased,although the level of phosphorylated phosphatase and tensin homolog increased,indicating that XB 130 activated the P展开更多
文摘This paper for the first time reveals high-resolution core records of Zabuye Salt Lake in the interior of the Qinghai-Tibet Plateau. According to 1346 samples taken continuously, relatively accurate 14^C, U-series disequilibrium and ESR ages have been obtained, thus revealing that the lake core ages from 0 to 83.63 m of hole SZK02 are -800 to over 128 ka. In the paper, the lake core sedimentary characteristics （including the lithologies and mineral assemblages） are analyzed in detail and correlated with ostracod assemblages I to XX and sporopollen zones A to I, and on the basis of an integrated analysis of the δ^18O values of authigenic calcium-magnesium carbonate and environmental proxies of minerals, sporopollen and microfossils in the lake core, a correlation has been made of oxygen isotope change between this lake core and the Greenland GISP2 and GRIP and Guliya ice cores, and the climate of Zabuye Salt Lake since 128 ka BP is divided into the last interglacial stage （including substages e, d, c, b and a） of oxygen isotope stage （OIS） 5, early glacial stadial of the last glacial stage of OIS 4, interglacial stadial of the last glacial stage of OIS 3, late glacial stadial of the last glacial stage or Last Glacial Maximum of OIS 2 and postglacial state of OIS 1; in addition, 6 Heinrich （H6-H1） events, Younger Dryas event and 8.2 ka BP cold event have been recognized.
文摘Background：XB 130 is a recently discovered adaptor protein that is highly expressed in many malignant tumors,but few studies have investigated its role in hepatocellular carcinoma （HCC）.Therefore,this study explored the relationship between this protein and liver cancer and investigated its molecular mechanism of action.Methods：The expression of XB 130 between HCC tissues and adjacent nontumor tissues was compared by real-time polymerase chain reaction,immunochemistry,and Western blotting.XB130 silencing was performed using small hairpin RNA.The effect of silencing XB130 was examined using Cell Counting Kit-8,colony assay,wound healing assay,and cell cycle analysis.Results：We found that XB 130 was highly expressed in HCC tissues （cancer tissues vs.adjacent tissues：0.23 ± 0.02 vs.0.17 ± 0.02,P 〈 0.05） and liver cancer cell lines,particularly MHCC97H and HepG2 （MHCC97H and HepG2 vs.normal liver cell line LO-2：2.35 ± 0.26 and 2.04 ± 0.04 vs.1.00 ± 0.04,respectively,all P 〈 0.05）.The Cell Counting Kit-8 assay,colony formation assay,and xenograft model in nude mice showed that silencing XB130 inhibited cell proliferative ability both in vivo and in vitro,with flow cytometry demonstrating that the cells were arrested in the G0/G1 phase in HepG2 （HepG2 XB130-silenced group [shA] vs.HepG2 scramble group [NA]：74.32 ± 5.86% vs.60.21 ± 3.07%,P 〈 0.05） and that the number of G2/M phase cells was decreased （HepG2 shA vs.HepG2 NA：8.06 ± 2.41% vs.18.36 ± 4.42%,P 〈 0.05）.Furthermore,the cell invasion and migration abilities were impaired,and the levels of the epithelial-mesenchymal transition-related indicators vimentin and N-cadherin were decreased,although the level of E-cadherin was increased after silencingXB130.Western blotting showed that the levels of phosphorylated phosphoinositide 3-kinase （PI3K） and phospho-protein kinase B （p-Akt） also increased,although the level of phosphorylated phosphatase and tensin homolog increased,indicating that XB 130 activated the P
