非典型的沙门菌是引起人类细菌性肠道炎常见的一个原因。血清学分离被广泛用作细分离沙门菌种的一种病原学分型方法。每个血清型都是通过细胞表面上结合的部分脂多糖(0抗原)和1种或2种鞭毛蛋白(H抗原),许多血清型有能力表达2种不同的H抗...非典型的沙门菌是引起人类细菌性肠道炎常见的一个原因。血清学分离被广泛用作细分离沙门菌种的一种病原学分型方法。每个血清型都是通过细胞表面上结合的部分脂多糖(0抗原)和1种或2种鞭毛蛋白(H抗原),许多血清型有能力表达2种不同的H抗原,然而个别只能表达1种H抗原自从90年代中期,肠道沙门菌亚种血清型4,[5],12 : i :-(S.4,[5],12 : i :-)引起的人类沙门菌病的发生率在欧洲、亚洲、北美和南美逐步增加[1-5]。展开更多
Salmonella enterica serovar 1,4,[5],12:i:-(S.1,4,[5],12:i:-)has been recognized as an emerging foodborne pathogen in recent years.It can cause human salmonellosis predominated by the contamination of animal-derived fo...Salmonella enterica serovar 1,4,[5],12:i:-(S.1,4,[5],12:i:-)has been recognized as an emerging foodborne pathogen in recent years.It can cause human salmonellosis predominated by the contamination of animal-derived foods such as raw poultry and pork.This study aimed to characterize the genetic diversity,plasmid replicon types,and antibiotic resistance of 15 S.1,4,[5],12:i:-environmental isolates collected from two poultry farms using pulsed-field gel electrophoresis(PFGE),multilocus sequence typing(MLST),polymerase chain reaction-based replicon typing,and minimum inhibitory concentration approach.Ten different PFGE genotypes were detected,indicating a high diversity among these S.1,4,[5],12:i:-isolates.Three sequence types(ST19,ST1544,ST34)were identified by MLST.Among them,ST1544 was first detected in S.1,4,[5],12:i:-environmental isolates from poultry farms.All isolates were resistant to cefazolin,cefotetan,tobramycin,amikacin,and gentamicin,but susceptible to piperacillin-tazobactam,aztreonam,ceftazidime,cefepime,and ertapenem.Five incompatibility groups(Inc)of plasmids were identified,including IncFIIs(66.7%),IncHI2(20%),IncI1(6.7%),IncN(6.7%),and IncQ(6.7%).Among these isolates,80%carried at least one plasmid replicon type,and 20%carried multiple plasmid replicon types.Interestingly,the multidrug-resistant isolate 263 carried numerous resistance genes(i.e.qnrS,aac(6ʹ)-Ib-cr,bla_(TEM),bla_(CTX-M-9),bla_(OXA-1),sul1,sul2,sul3,floR,and mcr-1)and class I integronase gene intI1,which possessed both IncHI2 and IncQ plasmids,suggesting that resistance genes may be horizontally transferred by the combination of IncHI2 and IncQ plasmids.Collectively,antibiotic-resistant S.1,4,[5],12:i:-isolates were first found in poultry farm environments in China,and surveillance should be strengthened to prevent their further spread from poultry farms to foods.展开更多
[目的]探讨后外侧人路微创小切口人工全髋关节置换术（total hip arthroplasty，THA）的临床疗效。[方法]2005年6月～2008年12月，采用改良后外侧入路微创小切口人工全髋关节置换术治疗髋关节疾病患者31例33髋，均为单切口微创技术，并...[目的]探讨后外侧人路微创小切口人工全髋关节置换术（total hip arthroplasty，THA）的临床疗效。[方法]2005年6月～2008年12月，采用改良后外侧入路微创小切口人工全髋关节置换术治疗髋关节疾病患者31例33髋，均为单切口微创技术，并与同时期微创传统人工全髋关节置换术的28例31髋患者比较，两组患者年龄、性别、病种、体重指数、髋关节功能等大致相同。分析两组病例围手术期出血量、手术时间、切口长度、疼痛评分、Harris评分、假体位置、并发症等，并进行两组病例对比研究。[结果]小切口THA组与传统THA组病例随访6～48个月，平均24．8个月。两组手术时间相近。小切口组平均切口长度9．2cm（7～11cm），传统手术组15cm（12—20cm），两组差异有统计学意义（P〈0．01）；围手术期出血量小切口组450ml（200～600m1），传统组800ml（600～1200m1），两组有统计学意义（P〈0．05）；小切口组疼痛评分5．1分，传统组为6．5分（P〈0．05）；术后6个月小切口组Harris评分93．8分（85～99分）；传统组92．1分（82～96分）（P〉0．05）。小切口组术后早期功能恢复较传统组快，而中期结果相似。术后及随访时，两组假体位置均良好。两组THA均未出现术后脱位、感染。肢体不等长各2例，但差异均在1cm以内，两组均各出现2例下肢深静脉血栓形成。[结论]小切口微创THA可选择性用于部分髋关节疾病的治疗，手术创伤小，围手术期出血少，切口小，疼痛轻，术后恢复快，且可获得与传统后路THA相同的治疗效果。展开更多
文摘非典型的沙门菌是引起人类细菌性肠道炎常见的一个原因。血清学分离被广泛用作细分离沙门菌种的一种病原学分型方法。每个血清型都是通过细胞表面上结合的部分脂多糖(0抗原)和1种或2种鞭毛蛋白(H抗原),许多血清型有能力表达2种不同的H抗原,然而个别只能表达1种H抗原自从90年代中期,肠道沙门菌亚种血清型4,[5],12 : i :-(S.4,[5],12 : i :-)引起的人类沙门菌病的发生率在欧洲、亚洲、北美和南美逐步增加[1-5]。
基金supported by the National Natural Science Foundation of China(No.32102111)the Project was funded by the China Postdoctoral Science Foundation(2021M702194).
文摘Salmonella enterica serovar 1,4,[5],12:i:-(S.1,4,[5],12:i:-)has been recognized as an emerging foodborne pathogen in recent years.It can cause human salmonellosis predominated by the contamination of animal-derived foods such as raw poultry and pork.This study aimed to characterize the genetic diversity,plasmid replicon types,and antibiotic resistance of 15 S.1,4,[5],12:i:-environmental isolates collected from two poultry farms using pulsed-field gel electrophoresis(PFGE),multilocus sequence typing(MLST),polymerase chain reaction-based replicon typing,and minimum inhibitory concentration approach.Ten different PFGE genotypes were detected,indicating a high diversity among these S.1,4,[5],12:i:-isolates.Three sequence types(ST19,ST1544,ST34)were identified by MLST.Among them,ST1544 was first detected in S.1,4,[5],12:i:-environmental isolates from poultry farms.All isolates were resistant to cefazolin,cefotetan,tobramycin,amikacin,and gentamicin,but susceptible to piperacillin-tazobactam,aztreonam,ceftazidime,cefepime,and ertapenem.Five incompatibility groups(Inc)of plasmids were identified,including IncFIIs(66.7%),IncHI2(20%),IncI1(6.7%),IncN(6.7%),and IncQ(6.7%).Among these isolates,80%carried at least one plasmid replicon type,and 20%carried multiple plasmid replicon types.Interestingly,the multidrug-resistant isolate 263 carried numerous resistance genes(i.e.qnrS,aac(6ʹ)-Ib-cr,bla_(TEM),bla_(CTX-M-9),bla_(OXA-1),sul1,sul2,sul3,floR,and mcr-1)and class I integronase gene intI1,which possessed both IncHI2 and IncQ plasmids,suggesting that resistance genes may be horizontally transferred by the combination of IncHI2 and IncQ plasmids.Collectively,antibiotic-resistant S.1,4,[5],12:i:-isolates were first found in poultry farm environments in China,and surveillance should be strengthened to prevent their further spread from poultry farms to foods.
文摘[目的]探讨后外侧人路微创小切口人工全髋关节置换术（total hip arthroplasty，THA）的临床疗效。[方法]2005年6月～2008年12月，采用改良后外侧入路微创小切口人工全髋关节置换术治疗髋关节疾病患者31例33髋，均为单切口微创技术，并与同时期微创传统人工全髋关节置换术的28例31髋患者比较，两组患者年龄、性别、病种、体重指数、髋关节功能等大致相同。分析两组病例围手术期出血量、手术时间、切口长度、疼痛评分、Harris评分、假体位置、并发症等，并进行两组病例对比研究。[结果]小切口THA组与传统THA组病例随访6～48个月，平均24．8个月。两组手术时间相近。小切口组平均切口长度9．2cm（7～11cm），传统手术组15cm（12—20cm），两组差异有统计学意义（P〈0．01）；围手术期出血量小切口组450ml（200～600m1），传统组800ml（600～1200m1），两组有统计学意义（P〈0．05）；小切口组疼痛评分5．1分，传统组为6．5分（P〈0．05）；术后6个月小切口组Harris评分93．8分（85～99分）；传统组92．1分（82～96分）（P〉0．05）。小切口组术后早期功能恢复较传统组快，而中期结果相似。术后及随访时，两组假体位置均良好。两组THA均未出现术后脱位、感染。肢体不等长各2例，但差异均在1cm以内，两组均各出现2例下肢深静脉血栓形成。[结论]小切口微创THA可选择性用于部分髋关节疾病的治疗，手术创伤小，围手术期出血少，切口小，疼痛轻，术后恢复快，且可获得与传统后路THA相同的治疗效果。
