Background:β-defensins have attracted considerable research interest because of their roles in protecting hosts from various pathogens.This study was conducted to investigate the expression profiles of the porcineβ-...Background:β-defensins have attracted considerable research interest because of their roles in protecting hosts from various pathogens.This study was conducted to investigate the expression profiles of the porcineβ-defensin114(PBD114)in different breeds and in response to infections.Moreover,the function of PBD114 protein was partially investigated.Methods:Six Tibetan pigs(TP)and six DLY(Duroc×Landrace×Yorkshire)pigs were slaughtered to explore the expression profiles of PBD114 in different breeds and tissues.For infection models,sixteen DLY pigs were divided into two groups and challenged either with sterile saline or E.coli K88.The recombinant protein PBD114(r PBD114)was obtained by using a heterologous expression system in E.coli.Results:PBD114 gene was highly expressed in tissues such as the intestine,liver,spleen,and thymus.Interestingly,the expression level of PBD114 gene was higher in the TP pigs than in the DLY pigs(P<0.05),and was significantly elevated upon E.coli K88 challenge(P<0.05).The nucleotide sequences of PBD114 from Tibetan and DLY pigs was identical,and both showed a 210-bp open reading frame encoding a 69-amino acid mature peptide.To explaore the function of PBD114 protein,PBD114 gene was successfully expressed in E.coli Origami B(DE3)and the molecular weight of the r PBD114 was estimated by SDS-PAGE to be 25 k Da.The r PBD114 was purified and mass spectrometry verified the protein as PBD114.Importantly,r PBD114 showed antimicrobial activities against E.coli DH5αand E.coli K88,and the minimal inhibitory concentrations(MICs)were 64 and 128μg/m L,respectively.Hemolytic and cytotoxicity assays showed that r PBD114 did not affect cell viability under physiological concentrations.Conclusions:PBD114 is an infection response gene that is differentially-expressed between different porcine breeds and tissues.The antimicrobial activity of PBD114 protein,against pathogens such as the E.coli K88,suggested that it may serve as a candidate for the substitution of conventionally used antibiotics.展开更多
The neutron flow model predicts that neutrons start to flow freely between the approaching nuclei ^58Fe and ^208 pb at s=3fm, a length in which the effective surfaces of these nuclei are 3 fm apart. As a result of neu...The neutron flow model predicts that neutrons start to flow freely between the approaching nuclei ^58Fe and ^208 pb at s=3fm, a length in which the effective surfaces of these nuclei are 3 fm apart. As a result of neutron flow, the N/Z value rapidly reaches an equilibrium distribution. Meanwhile the system, originally in the fusion valley, is injected into the asymmetric fission valley. The dynamic process of the composite nucleus in the asymmetric fission valley is treated with a two-parameter Smoluchowski equation. It is shown that the probability to overcome the asymmetric fission barrier and to achieve compound nucleus configuration, hence the fusion cross section is obviously suppressed due to the effect of isospin equilibrium.展开更多
Asteroseismology is a unique and powerful tool to investigate the internal structure of stars. CBS 114 is the sixth known pulsating DBV star. It was observed by Handler, Metcalfe, ＆ Wood at the South African Astronom...Asteroseismology is a unique and powerful tool to investigate the internal structure of stars. CBS 114 is the sixth known pulsating DBV star. It was observed by Handler, Metcalfe, ＆ Wood at the South African Astronomical Observatory over three weeks in 2001. Then, it was observed by Metcalfe et al. for seven nights （2004 Feb. 19-25） on the 1.8 m telescope at the Bohyunsan Optical Astronomy Observatory and seven nights （2004 Feb. 21-27） on the 2.1 m telescope at the McDonald Observatory. Totally two triplets, four doublets, and five singlets were identified. The frequency splitting values are very different, from 5.2 p.Hz to 11.9 ~tHz, which may reflect differential rotations. We evolve grids of white dwarf models by MESA. Cores, added with He/C envelopes, of those white dwarf models are inserted into WDEC to evolve grids of DBV star models. With those DBV star models, we calculate eigenperiods. Those calculated periods are used to fit observed periods. A best-fitting model is selected. The parameters are T^ff ----- 25 000 K, M, = 0.740 MG and log（MHe/M,） = -4.5. With the relatively large stellar mass, the effective temperature is close to the previous spectroscopic result. In addition, kinetic energy distributions are calculated for the best-fitting model. We find that the observed modes with large frequency splitting values are fitted by the calculated modes with a large amount of kinetic energy distributed in the C/O core. After preliminary analysis, we suggest that the C/O core may rotate at least two times faster than the helium layer for CBS 114.展开更多
114 cases of rheumatic arthritis were successfully treated with Jiang Chen Pian,a kind of tablets developed in our hospital. Data of the 114 cases in the treatment group collected from Oct. 1995 to Feb. 1997 and of th...114 cases of rheumatic arthritis were successfully treated with Jiang Chen Pian,a kind of tablets developed in our hospital. Data of the 114 cases in the treatment group collected from Oct. 1995 to Feb. 1997 and of the 68 cases in the control group showed that the therapeutic effects in the former were rather satisfactory. A report follows.展开更多
A method based on cloud point extraction was developed to determine phthalate esters including di-ethyl-phthalate （DEP）, di- （2-ethylhexyl）-phthalate （DEHP） and di-cyclohexyl-phthalate （DCP） in environmental w...A method based on cloud point extraction was developed to determine phthalate esters including di-ethyl-phthalate （DEP）, di- （2-ethylhexyl）-phthalate （DEHP） and di-cyclohexyl-phthalate （DCP） in environmental water samples using high-performance liquid chromatography separation and ultraviolet detection （HPLC-UV）. The non-ionic surfactant Triton X-114 was chosen as extraction solvent. The parameters affecting extraction efficiency, such as concentrations of Triton X-114 and Na2SO4, equilibration temperature, equilibration time and centrifugation time were evaluated and optimized. Under the optimum conditions, the method can achieve preconcentration factors of 35, 88, 111 and detection of limits of 2.0, 3.8, 1.0 ng/ml for DEP, DEHP and DCP in 10-ml water sample, respectively. The proposed method was successfully applied to the determination of trace amount of phathalate esters in effluent water of the wastewater treatment plant and the lixivium of plastic fragments.展开更多
基金supported by the Key Research and Development Program of Sichuan Province(2018NZDZX0005)the Youth Innovation Teams of Animal Feed Biotechnology of Sichuan Province(2016TD0028)
文摘Background:β-defensins have attracted considerable research interest because of their roles in protecting hosts from various pathogens.This study was conducted to investigate the expression profiles of the porcineβ-defensin114(PBD114)in different breeds and in response to infections.Moreover,the function of PBD114 protein was partially investigated.Methods:Six Tibetan pigs(TP)and six DLY(Duroc×Landrace×Yorkshire)pigs were slaughtered to explore the expression profiles of PBD114 in different breeds and tissues.For infection models,sixteen DLY pigs were divided into two groups and challenged either with sterile saline or E.coli K88.The recombinant protein PBD114(r PBD114)was obtained by using a heterologous expression system in E.coli.Results:PBD114 gene was highly expressed in tissues such as the intestine,liver,spleen,and thymus.Interestingly,the expression level of PBD114 gene was higher in the TP pigs than in the DLY pigs(P<0.05),and was significantly elevated upon E.coli K88 challenge(P<0.05).The nucleotide sequences of PBD114 from Tibetan and DLY pigs was identical,and both showed a 210-bp open reading frame encoding a 69-amino acid mature peptide.To explaore the function of PBD114 protein,PBD114 gene was successfully expressed in E.coli Origami B(DE3)and the molecular weight of the r PBD114 was estimated by SDS-PAGE to be 25 k Da.The r PBD114 was purified and mass spectrometry verified the protein as PBD114.Importantly,r PBD114 showed antimicrobial activities against E.coli DH5αand E.coli K88,and the minimal inhibitory concentrations(MICs)were 64 and 128μg/m L,respectively.Hemolytic and cytotoxicity assays showed that r PBD114 did not affect cell viability under physiological concentrations.Conclusions:PBD114 is an infection response gene that is differentially-expressed between different porcine breeds and tissues.The antimicrobial activity of PBD114 protein,against pathogens such as the E.coli K88,suggested that it may serve as a candidate for the substitution of conventionally used antibiotics.
基金Supported by the National Natural Science Foundation of China under Grants Nos 10235020 and 10235030, and the Major State Basic Research Development Programme under Grant No G200007400.
文摘The neutron flow model predicts that neutrons start to flow freely between the approaching nuclei ^58Fe and ^208 pb at s=3fm, a length in which the effective surfaces of these nuclei are 3 fm apart. As a result of neutron flow, the N/Z value rapidly reaches an equilibrium distribution. Meanwhile the system, originally in the fusion valley, is injected into the asymmetric fission valley. The dynamic process of the composite nucleus in the asymmetric fission valley is treated with a two-parameter Smoluchowski equation. It is shown that the probability to overcome the asymmetric fission barrier and to achieve compound nucleus configuration, hence the fusion cross section is obviously suppressed due to the effect of isospin equilibrium.
基金supported by the National Natural Science Foundation of China(Grant No.11563001)the Research Fund of Chuxiong Normal University(XJGG1501)+1 种基金the Collaborating Research Program of Key Laboratory for the Structure and Evolution of Celestial Objects,Chinese Academy of Sciences(OP201502)the Yunnan Applied Basic Research Project(2015FD044)
文摘Asteroseismology is a unique and powerful tool to investigate the internal structure of stars. CBS 114 is the sixth known pulsating DBV star. It was observed by Handler, Metcalfe, ＆ Wood at the South African Astronomical Observatory over three weeks in 2001. Then, it was observed by Metcalfe et al. for seven nights （2004 Feb. 19-25） on the 1.8 m telescope at the Bohyunsan Optical Astronomy Observatory and seven nights （2004 Feb. 21-27） on the 2.1 m telescope at the McDonald Observatory. Totally two triplets, four doublets, and five singlets were identified. The frequency splitting values are very different, from 5.2 p.Hz to 11.9 ~tHz, which may reflect differential rotations. We evolve grids of white dwarf models by MESA. Cores, added with He/C envelopes, of those white dwarf models are inserted into WDEC to evolve grids of DBV star models. With those DBV star models, we calculate eigenperiods. Those calculated periods are used to fit observed periods. A best-fitting model is selected. The parameters are T^ff ----- 25 000 K, M, = 0.740 MG and log（MHe/M,） = -4.5. With the relatively large stellar mass, the effective temperature is close to the previous spectroscopic result. In addition, kinetic energy distributions are calculated for the best-fitting model. We find that the observed modes with large frequency splitting values are fitted by the calculated modes with a large amount of kinetic energy distributed in the C/O core. After preliminary analysis, we suggest that the C/O core may rotate at least two times faster than the helium layer for CBS 114.
文摘114 cases of rheumatic arthritis were successfully treated with Jiang Chen Pian,a kind of tablets developed in our hospital. Data of the 114 cases in the treatment group collected from Oct. 1995 to Feb. 1997 and of the 68 cases in the control group showed that the therapeutic effects in the former were rather satisfactory. A report follows.
基金Projected supported by the National Basic Research Program (973)of China (No. 2003CB415001)the Pilot Program of KnowledgeInnovation Program of Chinese Academy of Sciences (No. KZCX3-SW-431).
文摘A method based on cloud point extraction was developed to determine phthalate esters including di-ethyl-phthalate （DEP）, di- （2-ethylhexyl）-phthalate （DEHP） and di-cyclohexyl-phthalate （DCP） in environmental water samples using high-performance liquid chromatography separation and ultraviolet detection （HPLC-UV）. The non-ionic surfactant Triton X-114 was chosen as extraction solvent. The parameters affecting extraction efficiency, such as concentrations of Triton X-114 and Na2SO4, equilibration temperature, equilibration time and centrifugation time were evaluated and optimized. Under the optimum conditions, the method can achieve preconcentration factors of 35, 88, 111 and detection of limits of 2.0, 3.8, 1.0 ng/ml for DEP, DEHP and DCP in 10-ml water sample, respectively. The proposed method was successfully applied to the determination of trace amount of phathalate esters in effluent water of the wastewater treatment plant and the lixivium of plastic fragments.
