[Objective] Effects of nitrogen amount and nitrogen form on 1-deoxynojimycin（DNJ）content in mulberry leaf was explored in order to provide a reference for improving DNJ content in mulberry leaf and its medicinal val...[Objective] Effects of nitrogen amount and nitrogen form on 1-deoxynojimycin（DNJ）content in mulberry leaf was explored in order to provide a reference for improving DNJ content in mulberry leaf and its medicinal value.[Method] Effects of nitrogen amount（NO-3-N and NH＋4-N）and nitrogen form（NO-3-N/NH＋4-N）on DNJ content in mulberry leaf were studied by changing nitrogen amount and nitrogen form in water culture solution.[Result] DNJ contents in mulberry leaf both increased at first and then decreased with the increase of nitrogen amount,furthermore compared with NH＋4-N,NO-3-N was more beneficial to the accumulation of DNJ;DNJ content in mulberry leaf obviously increased at first and then decreased with the decrease of NO-3-N/NH＋4-N,among them when its rate was 25/75,DNJ content reached the highest.[Conclusion] Proper nitrogen amount and nitrogen form could effectively improve the DNJ content in mulberry leaf.展开更多
[Objective] This study aimed to scientifically improve the 1-deoxynojimycin （DNJ） yield of mulberry leaves in spring. [Method] ＂3414＂ experimental design was adopted to investigate the effects of different fertili...[Objective] This study aimed to scientifically improve the 1-deoxynojimycin （DNJ） yield of mulberry leaves in spring. [Method] ＂3414＂ experimental design was adopted to investigate the effects of different fertilizer application rates of nitrogen, phosphorus and potassium on the DNJ content in mulberry leaves. The regression relationship was further simulated with DNJ yield as the objective function and fertil- izer application rates of nitrogen, phosphorus and potassium as the regulatory factors. [Result] The results showed that nitrogen, phosphorus and potassium all have great effects on the DNJ content in mulberry leaves at different parts of mulberry tree （Morus alba）; the DNJ content in mulberry leaves increases with the increasing single-factor fertilizer application rate and reaches the maximum under N2P2K2 level; different fertilizer application rates and combinations of nitrogen, phosphorus and potassium can significantly affect the DNJ content in mulberry leaves; according to the simulated fertilizer effect function, the optimal economic yield of total DNJ in mul- berry leaves was 23.23 kg/hm^2, the recommended fertilization indicators were 286.11 kg/hm^2 N, 95.22 kg/hm^2 P2O5 and 159.62 kg/hm^2 K20. [Conclusion] After confirmation in productive practice, this optimal fertilization program can provide reference for the construction of mulberry plantation with high yield and high quality of DNJ.展开更多
Ten mulberry species were taken as materials to detect contents of DNJ,polysaccharide and flavonoid.The results indicated that the three active components differed significantly upon species.Hongguo 2 and Shushen 1,wh...Ten mulberry species were taken as materials to detect contents of DNJ,polysaccharide and flavonoid.The results indicated that the three active components differed significantly upon species.Hongguo 2 and Shushen 1,which are of high contents of active components,were selected as materials to measure contents change of active components in fresh fruits during ripening process after full-bloom stage.The research suggested that DNJ mass fractions of Hongguo 2 and Shushen 1 on the 3rd d in full-bloom stage were 0.074 1% and 0.072 5%;DNJ declined gradually with growth of the fruit and DNJ contents in the two cultivars decreased by 49.80% and 58.90% on the 31st d in full-bloom stage,compared with the 3rd d.As mulberry grew,polysaccharide contents in Hongguo 2 and Shushen 1 were increasing and achieved peaks on the 27th d in full-bloom stage at 9.570% and 10.276%,respectively.For total flavonoids,the contents in Hongguo 2 and Shushen 1 were both of two peaks on 11th d and 27th d.The mass fractions were 0.313%,0.444%,and 0.264%,0.326%,accordingly.In addition,DNJ contents in ripe seeds of Hongguo 2 and Shushen 1 were significantly higher than those in fruit,but total flavonoid contents were lower.Polysaccharide was not detected.It can be concluded that mulberry fruits growing for 27 d can be either as excellent food or for medicine.展开更多
基金Supported by Key Technology Innovation Project of Sichuan Province(2008NC00365)~~
文摘[Objective] Effects of nitrogen amount and nitrogen form on 1-deoxynojimycin（DNJ）content in mulberry leaf was explored in order to provide a reference for improving DNJ content in mulberry leaf and its medicinal value.[Method] Effects of nitrogen amount（NO-3-N and NH＋4-N）and nitrogen form（NO-3-N/NH＋4-N）on DNJ content in mulberry leaf were studied by changing nitrogen amount and nitrogen form in water culture solution.[Result] DNJ contents in mulberry leaf both increased at first and then decreased with the increase of nitrogen amount,furthermore compared with NH＋4-N,NO-3-N was more beneficial to the accumulation of DNJ;DNJ content in mulberry leaf obviously increased at first and then decreased with the decrease of NO-3-N/NH＋4-N,among them when its rate was 25/75,DNJ content reached the highest.[Conclusion] Proper nitrogen amount and nitrogen form could effectively improve the DNJ content in mulberry leaf.
基金Supported by Crop Breeding Research Project of Sichuan Province of the 12th Five-Year Plan(2011JYGC10-027-02)Excellent Paper Fund from Sichuan Academy of Agricultural Sciences(No.2011LWJJ-008)~~
文摘[Objective] This study aimed to scientifically improve the 1-deoxynojimycin （DNJ） yield of mulberry leaves in spring. [Method] ＂3414＂ experimental design was adopted to investigate the effects of different fertilizer application rates of nitrogen, phosphorus and potassium on the DNJ content in mulberry leaves. The regression relationship was further simulated with DNJ yield as the objective function and fertil- izer application rates of nitrogen, phosphorus and potassium as the regulatory factors. [Result] The results showed that nitrogen, phosphorus and potassium all have great effects on the DNJ content in mulberry leaves at different parts of mulberry tree （Morus alba）; the DNJ content in mulberry leaves increases with the increasing single-factor fertilizer application rate and reaches the maximum under N2P2K2 level; different fertilizer application rates and combinations of nitrogen, phosphorus and potassium can significantly affect the DNJ content in mulberry leaves; according to the simulated fertilizer effect function, the optimal economic yield of total DNJ in mul- berry leaves was 23.23 kg/hm^2, the recommended fertilization indicators were 286.11 kg/hm^2 N, 95.22 kg/hm^2 P2O5 and 159.62 kg/hm^2 K20. [Conclusion] After confirmation in productive practice, this optimal fertilization program can provide reference for the construction of mulberry plantation with high yield and high quality of DNJ.
基金Supported by Special Fund of Major Industry Technology of Sichuan Economic and Trade Commission(No.2008NC00365)National Spark Program(No.2008GA81002)+1 种基金Special Fund of Sichuan Financial Genetic Engineering(No.2011JYGC-QNJJ-021)Excellent Paper Fund of Sichuan Academy of Agricultural Sciences(No.2011JYGC-LWJJ-07)~~
文摘Ten mulberry species were taken as materials to detect contents of DNJ,polysaccharide and flavonoid.The results indicated that the three active components differed significantly upon species.Hongguo 2 and Shushen 1,which are of high contents of active components,were selected as materials to measure contents change of active components in fresh fruits during ripening process after full-bloom stage.The research suggested that DNJ mass fractions of Hongguo 2 and Shushen 1 on the 3rd d in full-bloom stage were 0.074 1% and 0.072 5%;DNJ declined gradually with growth of the fruit and DNJ contents in the two cultivars decreased by 49.80% and 58.90% on the 31st d in full-bloom stage,compared with the 3rd d.As mulberry grew,polysaccharide contents in Hongguo 2 and Shushen 1 were increasing and achieved peaks on the 27th d in full-bloom stage at 9.570% and 10.276%,respectively.For total flavonoids,the contents in Hongguo 2 and Shushen 1 were both of two peaks on 11th d and 27th d.The mass fractions were 0.313%,0.444%,and 0.264%,0.326%,accordingly.In addition,DNJ contents in ripe seeds of Hongguo 2 and Shushen 1 were significantly higher than those in fruit,but total flavonoid contents were lower.Polysaccharide was not detected.It can be concluded that mulberry fruits growing for 27 d can be either as excellent food or for medicine.
