Coupling reaction of 4-chloro-7-H-pyrrolo[2,3-d]pyrimidine with 2,3,5-tri-O-acetyl -β-D-ribofuranosyl chloride under the basic condition was investigated. An abnormal coupling reaction, in which the heterocyclic base...Coupling reaction of 4-chloro-7-H-pyrrolo[2,3-d]pyrimidine with 2,3,5-tri-O-acetyl -β-D-ribofuranosyl chloride under the basic condition was investigated. An abnormal coupling reaction, in which the heterocyclic base attacked at the carbon of 1,2-O-methylidene moiety instead of anomeric carbon of ribose was observed and the structure of products 5a, 5b were identified by NMR and X-Ray diffraction.展开更多
目的:了解胸腺基质淋巴细胞生成素(TSLP)在1-氯-2,4-二硝基苯(DNCB)致敏诱导皮炎小鼠中的表达,并评价TSLP在小鼠皮炎免疫反应中的作用。方法:将20只11周龄SPF级BALB/c小鼠随机分为2组(每组10只):致敏组先后接受含1.0%和0.1%DNCB的橄榄...目的:了解胸腺基质淋巴细胞生成素(TSLP)在1-氯-2,4-二硝基苯(DNCB)致敏诱导皮炎小鼠中的表达,并评价TSLP在小鼠皮炎免疫反应中的作用。方法:将20只11周龄SPF级BALB/c小鼠随机分为2组(每组10只):致敏组先后接受含1.0%和0.1%DNCB的橄榄油丙酮(4∶1)溶液反复刺激背部皮肤,对照组接受基质溶液(橄榄油∶丙酮=4∶1)涂抹背部皮肤。实验期间观察小鼠皮炎症状,监测小鼠挠痒次数和皮炎症状评分,观察皮肤组织病理改变。21 d后检测2组血浆总Ig E水平;利用实时荧光定量聚合酶链式反应(q PCR)法检测皮肤、肝脏、脾脏和肾脏组织中TSLP m RNA水平,采用western-blot法分析TSLP蛋白表达情况。结果:2组小鼠的抓挠次数和皮炎症状评分分别在致敏第8天和第6天后出现差异(P<0.05);致敏组小鼠血浆总Ig E水平[(132.61±14.89)μg/L]较对照组[(109.13±2.51)μg/L]显著升高(t=4.92,P<0.05)。与对照组(1)相比,致敏组皮肤(1.83)、脾脏(1.06)和肾脏(1.10)组织中TSLP m RNA相对表达量均升高,而肝脏(0.90)组织TSLP m RNA相对表达量降低,但组间比较差异均无统计学意义(P均>0.05)。Western-blot结果示TSLP蛋白在上述组织中的表达均无差异。结论:TSLP在DNCB致敏诱导皮炎小鼠的皮肤、肝脏、脾脏和肾脏组织中表达均无明显差异,其在小鼠皮炎免疫反应中的作用仍需进一步的研究。展开更多
基金This work was supported by the National Natural Science Foundation of China.
文摘Coupling reaction of 4-chloro-7-H-pyrrolo[2,3-d]pyrimidine with 2,3,5-tri-O-acetyl -β-D-ribofuranosyl chloride under the basic condition was investigated. An abnormal coupling reaction, in which the heterocyclic base attacked at the carbon of 1,2-O-methylidene moiety instead of anomeric carbon of ribose was observed and the structure of products 5a, 5b were identified by NMR and X-Ray diffraction.
文摘目的:了解胸腺基质淋巴细胞生成素(TSLP)在1-氯-2,4-二硝基苯(DNCB)致敏诱导皮炎小鼠中的表达,并评价TSLP在小鼠皮炎免疫反应中的作用。方法:将20只11周龄SPF级BALB/c小鼠随机分为2组(每组10只):致敏组先后接受含1.0%和0.1%DNCB的橄榄油丙酮(4∶1)溶液反复刺激背部皮肤,对照组接受基质溶液(橄榄油∶丙酮=4∶1)涂抹背部皮肤。实验期间观察小鼠皮炎症状,监测小鼠挠痒次数和皮炎症状评分,观察皮肤组织病理改变。21 d后检测2组血浆总Ig E水平;利用实时荧光定量聚合酶链式反应(q PCR)法检测皮肤、肝脏、脾脏和肾脏组织中TSLP m RNA水平,采用western-blot法分析TSLP蛋白表达情况。结果:2组小鼠的抓挠次数和皮炎症状评分分别在致敏第8天和第6天后出现差异(P<0.05);致敏组小鼠血浆总Ig E水平[(132.61±14.89)μg/L]较对照组[(109.13±2.51)μg/L]显著升高(t=4.92,P<0.05)。与对照组(1)相比,致敏组皮肤(1.83)、脾脏(1.06)和肾脏(1.10)组织中TSLP m RNA相对表达量均升高,而肝脏(0.90)组织TSLP m RNA相对表达量降低,但组间比较差异均无统计学意义(P均>0.05)。Western-blot结果示TSLP蛋白在上述组织中的表达均无差异。结论:TSLP在DNCB致敏诱导皮炎小鼠的皮肤、肝脏、脾脏和肾脏组织中表达均无明显差异,其在小鼠皮炎免疫反应中的作用仍需进一步的研究。