To investigate the effect of Yikun Neiyi Wan （益坤内异丸YKNYW） and gestrinone on the expression of aromatase P450 （P450arom）, cyclo-oxygenase-2 （COX-2） and estrogen receptor （ER） in isolated ectopic and normal...To investigate the effect of Yikun Neiyi Wan （益坤内异丸YKNYW） and gestrinone on the expression of aromatase P450 （P450arom）, cyclo-oxygenase-2 （COX-2） and estrogen receptor （ER） in isolated ectopic and normal endometriat stroma cells in vitro. Methods： Digestion and serial filtration were used to isolate and culture the ectopic and eutopic endometrial cells from patients with chocolate cyst in virto Transformation of the cell morphology was observed in a inverted microscope. The effect of YKNYW on the expression of aromatase P450, cyclo-oxygenase-2, estrogen receptor in cultured endometriosis cells were detected by immunohistochemical method. Results： The expression levels of P450arom, COX-2 in glandular epithelium cells in vitro were decreased significantly by YKNYW compared with gestrinone （P〈0.05）. ER expression in mesenchymal cells of endometriosis was increased by YKNYW in the large and medium dosage groups compared with gestrinone. Conclusion： The mechanism by which YKNYW alleviates endometriosis pain is possibly related to the decrease in ectopic endometrial P450 arom and COX-2 expression in glandular epithelium, contrary to gestrinone, and the increase in ER expression in mesenchymalis, consistent with gestrione in patients with endometriosis.展开更多
Cytochrome P450 aromatase(P450arom)plays a pivotal role in ovary development.In this study,we used semi-quanti-tative reverse transcription PCR(RT-PCR)to analyze spatiotemporal expressions of two P450arom genes(CYP19A...Cytochrome P450 aromatase(P450arom)plays a pivotal role in ovary development.In this study,we used semi-quanti-tative reverse transcription PCR(RT-PCR)to analyze spatiotemporal expressions of two P450arom genes(CYP19A and CYP19B)and their responses to temperature stress in female yellow catfish(Pelteobagrus fulvidraco).Tissue distribution pattern of CYP19 showed that CYP19B was abundantly expressed in fish brain and ovary(brain > ovary),but weakly in intestines,whereas CYP19A was exclusively expressed in ovary.Semi-quantitative RT-PCR analyses showed high transcript abundance of both CYP19A and CYP19B in the ovarian reproductive cycle,corresponding with serum estradiol-17β(E2)levels.Increases in aromatases,serum E2 and testosterone(T)levels in fish exposed to higher temperature indicated stimulation of ovarian maturation and recrudescence by heat stress in stages Ⅱ and Ⅴ during the ovarian cycle,whereas associated decreases in stage Ⅲ suggested vitellogenesis inhibition by heat stress.Gene expression of CYP19 was closely related to levels of serum E2.Results demonstrated CYP19 played a crucial role in the reproductive cycle of female yellow catfish.Different temperature stress affected CYP19 gene expression in the fish ovarian re-productive cycle.Associated P450arom genes could be useful for studying physiological aspects of yellow catfish.展开更多
文摘To investigate the effect of Yikun Neiyi Wan （益坤内异丸YKNYW） and gestrinone on the expression of aromatase P450 （P450arom）, cyclo-oxygenase-2 （COX-2） and estrogen receptor （ER） in isolated ectopic and normal endometriat stroma cells in vitro. Methods： Digestion and serial filtration were used to isolate and culture the ectopic and eutopic endometrial cells from patients with chocolate cyst in virto Transformation of the cell morphology was observed in a inverted microscope. The effect of YKNYW on the expression of aromatase P450, cyclo-oxygenase-2, estrogen receptor in cultured endometriosis cells were detected by immunohistochemical method. Results： The expression levels of P450arom, COX-2 in glandular epithelium cells in vitro were decreased significantly by YKNYW compared with gestrinone （P〈0.05）. ER expression in mesenchymal cells of endometriosis was increased by YKNYW in the large and medium dosage groups compared with gestrinone. Conclusion： The mechanism by which YKNYW alleviates endometriosis pain is possibly related to the decrease in ectopic endometrial P450 arom and COX-2 expression in glandular epithelium, contrary to gestrinone, and the increase in ER expression in mesenchymalis, consistent with gestrione in patients with endometriosis.
基金supported by the Key Open Fund of the Key Open Laboratory of Genetic BreedingAquaculture Biology of Aquatic Animal, Ministry of Agriculture, China (BZ200709)
文摘Cytochrome P450 aromatase(P450arom)plays a pivotal role in ovary development.In this study,we used semi-quanti-tative reverse transcription PCR(RT-PCR)to analyze spatiotemporal expressions of two P450arom genes(CYP19A and CYP19B)and their responses to temperature stress in female yellow catfish(Pelteobagrus fulvidraco).Tissue distribution pattern of CYP19 showed that CYP19B was abundantly expressed in fish brain and ovary(brain > ovary),but weakly in intestines,whereas CYP19A was exclusively expressed in ovary.Semi-quantitative RT-PCR analyses showed high transcript abundance of both CYP19A and CYP19B in the ovarian reproductive cycle,corresponding with serum estradiol-17β(E2)levels.Increases in aromatases,serum E2 and testosterone(T)levels in fish exposed to higher temperature indicated stimulation of ovarian maturation and recrudescence by heat stress in stages Ⅱ and Ⅴ during the ovarian cycle,whereas associated decreases in stage Ⅲ suggested vitellogenesis inhibition by heat stress.Gene expression of CYP19 was closely related to levels of serum E2.Results demonstrated CYP19 played a crucial role in the reproductive cycle of female yellow catfish.Different temperature stress affected CYP19 gene expression in the fish ovarian re-productive cycle.Associated P450arom genes could be useful for studying physiological aspects of yellow catfish.
