Cell-PLoc 2.0 is a package of web-servers evolved from Cell-PLoc (Chou, K.C. & Shen, H.B., Nature Protocols, 2008, 2:153-162) by a top-down approach to improve the power for predicting subcellular localization of ...Cell-PLoc 2.0 is a package of web-servers evolved from Cell-PLoc (Chou, K.C. & Shen, H.B., Nature Protocols, 2008, 2:153-162) by a top-down approach to improve the power for predicting subcellular localization of proteins in various organisms. It contains six predictors: Euk-mPLoc 2.0, Hum-mPLoc 2.0, Plant-mPLoc, Gpos-mPLoc, Gneg-mPLoc, and Virus-mPLoc, specialized for eukaryotic, human, plant, Gram- positive bacterial, Gram-negative bacterial, and virus proteins, respectively. Compared with Cell-PLoc, the predictors in the Cell-PLoc 2.0 have the following advantageous features: (1) they all have the capacity to deal with the multiplex proteins that can simultaneiously exist, or move between, two or more subcellular location sites;(2) no accession number is needed for the input of a query protein even if using the “high- level” GO (gene ontology) prediction engine;(3) the functional domain information and sequential evolution information are fused into the “ab initio” sequence-based prediction engine to enhance its accuracy. In this protocol, a step- to-step guide is provided for how to use the web server predictors in the Cell-PLoc 2.0 package, which is freely accessible to the public at http://www.csbio.sjtu.edu.cn/bioinf/Cell-PLoc-2/.展开更多
通过中药系统药理学数据库与分析平台(traditional Chinese medicine system pharmacology datebase and analysis platform,TCMSP)筛选五苓散组分白术、茯苓、猪苓、泽泻、桂枝的潜在活性成分,PharmMapper服务器预测潜在靶点,构建化合...通过中药系统药理学数据库与分析平台(traditional Chinese medicine system pharmacology datebase and analysis platform,TCMSP)筛选五苓散组分白术、茯苓、猪苓、泽泻、桂枝的潜在活性成分,PharmMapper服务器预测潜在靶点,构建化合物-靶点网络、蛋白互作(protein-protein interaction,PPI)网络,进行基因本体(gene ontology,GO)富集分析、基于KEGG(Kyoto encyclopedia of genes and enomes)的生物通路富集分析。结果表明,筛选得到五苓散中50种活性成分,构建化合物-靶点网络共459个节点,关键靶点包括CSDE1、PRPS1、HSD17B4、CLPP、ACBD7、AZGP1等。构建五苓散靶点的PPI互作网络包含155个节点和527条相互作用关系,关键节点包括CSDE1、CITED2、RANBP2、HSD17B4、TAF13、SOD2等。GO富集分析途径相关条目主要涉及细胞溶质、细胞对有机物质和化学刺激的反应,含氮物质代谢,有机物循环代谢进程,蛋白、小分子和离子结合及转运活性等。KEGG代谢通路分析包括膀胱癌、前列腺癌、内分泌抵抗、癌症中的蛋白聚糖、流体剪切应力和动脉粥样硬化等途径。研究结果初步验证了五苓散药理作用的分子机制,为进一步深入揭示其分子作用机制奠定了基础,预测五苓散的潜在作用靶点如PRPS1、HSD17B4等,可为五苓散的深入研究提供新思路。展开更多
Over the span of years, improvements over various synthesis methods of graphene are constantly pursued to provide safer and more effective alternatives. Though the extraction of graphene through Hummers method is one ...Over the span of years, improvements over various synthesis methods of graphene are constantly pursued to provide safer and more effective alternatives. Though the extraction of graphene through Hummers method is one of the oldest techniques yet it is one of the most suitable methods for the formation of bulk graphene. Graphene can be obtained in the form of reduced Graphite oxide, sometimes also referred as Graphene oxide. The effectiveness of this oxidation process can be evaluated by the magnitude of carbon/oxygen ratio of the obtained graphene. Here, graphene oxide (GO) was prepared by oxidizing the purified natural flake graphite (NFG) by a modified Hummers method. The attempts have been made to synthesize GO having few layers by using a modified Hummers method where the amount of NaNO3 has been decreased, and the amount of KMnO4 is increased. The reaction has been performed in a 9:1 (by volume) mixture of H2SO4/H3PO4. This modification is successful in increasing the reaction yield and reducing the toxic gas evolution while using a varied proportion of KMnO4 and H2SO4 as those required by Hummers method. A new component of K2S2O8 has been introduced to the reaction system to maintain the pH value. Reduced graphene oxide (rGO) was thereafter extracted by thermal modification of GO. Here, GO has been used as a precursor for graphene synthesis by thermal reduction processes. The results of FTIR and Raman spectroscopy analysis show that the NFG when oxidized by strong oxidants like KMnO4 and NaNO3, introduced oxygen atoms into the graphite layers and formed bonds like C=O, C-H, COOH and C-O-C with the carbon atoms in the graphite layers. The structure and morphology of both GO and rGO were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy, Raman spectroscopy, Brunauer-Emmett-Teller (BET) surface area analysis and differential scanning calorimetry (展开更多
研究探讨内隐联想测验(Implicit Association Test,IAT)和Go/No-go联想任务测验(Go/No-go AssociationTask,GNAT)测量大学生感恩的可行性,并分析内隐感恩与外显感恩的关系。预备性研究从现代汉语字典中挑选自我词/他人词各16个,从汉语...研究探讨内隐联想测验(Implicit Association Test,IAT)和Go/No-go联想任务测验(Go/No-go AssociationTask,GNAT)测量大学生感恩的可行性,并分析内隐感恩与外显感恩的关系。预备性研究从现代汉语字典中挑选自我词/他人词各16个,从汉语成语大辞典中挑选出与感恩相关的积极词/消极词各20个,根据65位大学生的评定结果筛选出自我词/他人词各8个,积极词/消极词各8个,用于正式研究。正式研究采用IAT、GNAT感恩测量程序和青少年感恩量表(Adolescent Gratitude Scale,AGS),对155名大学生同时进行IAT、GNAT及外显感恩的测量。结果发现:(1)大学生普遍具有积极的内隐感恩,内隐感恩不存在性别、生源地和独生与否等人口统计学指标上的显著差异;(2)大学生的内隐感恩与外显感恩相关不显著,是两个不同的建构;(3)采用IAT和GNAT研究感恩是可行的。展开更多
Graphene oxide and Ni-Al layered double hydroxides(GO@LDH) nanocomposites were synthesized via a one-pot hydrothermal process,and characterized by X-ray diffraction(XRD),Fourier transformed infrared spectroscopy(FTIR)...Graphene oxide and Ni-Al layered double hydroxides(GO@LDH) nanocomposites were synthesized via a one-pot hydrothermal process,and characterized by X-ray diffraction(XRD),Fourier transformed infrared spectroscopy(FTIR),scanning electron microscopy(SEM),transmission electron microscopy(TEM),X-ray photoelectron spectroscopy(XPS) and Raman spectroscopy in detail.The exploration of U(VI) sorption on GO@LDH surface was performed as a function of ionic strength,solution pH,contact time,U(VI) initial concentrations and temperature.Results of Langmuir isotherms showed that the sorption capacity of GO@LDH(160 mg/g) was much higher than those of LDH(69 mg/g) and GO(92 mg/g).The formed surface complexes between surface oxygen-containing functional groups of GO@LDH and U(VI) turned out to be the interaction mechanism of U(VI) with GO@LDH.According to the thermodynamic studies results,the sorption interaction was actually a spontaneous and endothermic chemical process.The sorption isotherms were better fitted with the Langmuir model compared with other models,which suggested the interaction was mainly dominated by mono layer coverage.The GO@LDH nanocomposites provide potential applications as adsorbents in the enrichment of radionuclides from wastewater in nuclear waste management and environmental remediation.展开更多
We prepared the nano-inorganic phase-change ＂alloy＂ materials through the modification of Na2SO4·10H2O using Na2HPO4·12H2O and GO nano-nucleating agent, and further investigated their thermophysical proper...We prepared the nano-inorganic phase-change ＂alloy＂ materials through the modification of Na2SO4·10H2O using Na2HPO4·12H2O and GO nano-nucleating agent, and further investigated their thermophysical properties such as melting/solidification temperatures and enthalpies via differential scanning calorimetry. When the weight ratio of Na2SO4·10H2O and Na2HPO4·12H2O was 8：2 and the weight ratio of graphene oxide was 0.5% of phase change material, the phase change ＂alloy＂ material showed excellent performances, specifically, the melting temperature and latent heat were found to be 22 ℃ and 190 J/g with a degree of subcooling decreased from 8.6℃ to 2.1℃. In order to extend the application of the phase change ＂alloy＂ material to building energy saving field, it was adsorbed on expanded glass beads under vacuum and further covered with diatomite. When the adsorption rate of EGB（volume） and PCAM（weight） was 2.5：1, the particle size of diatomaceous earth was found to be 3.6μm, while the best packaging result was obtained with the melting temperature and latent heat being 21℃ and 135 J/g, and no leakage was observed.展开更多
Graphene oxide(GO)-chitosan composite hydrogels were successfully prepared via the self-assembly of chitosan molecules and GO. These as-prepared hydrogels were characterized by different techniques. The morphology of ...Graphene oxide(GO)-chitosan composite hydrogels were successfully prepared via the self-assembly of chitosan molecules and GO. These as-prepared hydrogels were characterized by different techniques. The morphology of the internal network structure of the nanocomposite hydrogels was investigated. The adsorption capacity results demonstrate that the prepared GObased composite hydrogels can efficiently remove three tested dye molecules, Congo red, methylene blue and Rhodamine B, from wastewater in accordance with the pseudo-second-order model. The dye adsorption capacity of the obtained hydrogels is mainly attributed to the GO sheets, whereas the chitosan molecule was incorporated to facilitate the gelation process of the GO sheets. The present study indicates that the as-prepared composite hydrogels can serve as good adsorbents for wastewater treatment as well as the removal of harmful dyes.展开更多
文摘Cell-PLoc 2.0 is a package of web-servers evolved from Cell-PLoc (Chou, K.C. & Shen, H.B., Nature Protocols, 2008, 2:153-162) by a top-down approach to improve the power for predicting subcellular localization of proteins in various organisms. It contains six predictors: Euk-mPLoc 2.0, Hum-mPLoc 2.0, Plant-mPLoc, Gpos-mPLoc, Gneg-mPLoc, and Virus-mPLoc, specialized for eukaryotic, human, plant, Gram- positive bacterial, Gram-negative bacterial, and virus proteins, respectively. Compared with Cell-PLoc, the predictors in the Cell-PLoc 2.0 have the following advantageous features: (1) they all have the capacity to deal with the multiplex proteins that can simultaneiously exist, or move between, two or more subcellular location sites;(2) no accession number is needed for the input of a query protein even if using the “high- level” GO (gene ontology) prediction engine;(3) the functional domain information and sequential evolution information are fused into the “ab initio” sequence-based prediction engine to enhance its accuracy. In this protocol, a step- to-step guide is provided for how to use the web server predictors in the Cell-PLoc 2.0 package, which is freely accessible to the public at http://www.csbio.sjtu.edu.cn/bioinf/Cell-PLoc-2/.
文摘通过中药系统药理学数据库与分析平台(traditional Chinese medicine system pharmacology datebase and analysis platform,TCMSP)筛选五苓散组分白术、茯苓、猪苓、泽泻、桂枝的潜在活性成分,PharmMapper服务器预测潜在靶点,构建化合物-靶点网络、蛋白互作(protein-protein interaction,PPI)网络,进行基因本体(gene ontology,GO)富集分析、基于KEGG(Kyoto encyclopedia of genes and enomes)的生物通路富集分析。结果表明,筛选得到五苓散中50种活性成分,构建化合物-靶点网络共459个节点,关键靶点包括CSDE1、PRPS1、HSD17B4、CLPP、ACBD7、AZGP1等。构建五苓散靶点的PPI互作网络包含155个节点和527条相互作用关系,关键节点包括CSDE1、CITED2、RANBP2、HSD17B4、TAF13、SOD2等。GO富集分析途径相关条目主要涉及细胞溶质、细胞对有机物质和化学刺激的反应,含氮物质代谢,有机物循环代谢进程,蛋白、小分子和离子结合及转运活性等。KEGG代谢通路分析包括膀胱癌、前列腺癌、内分泌抵抗、癌症中的蛋白聚糖、流体剪切应力和动脉粥样硬化等途径。研究结果初步验证了五苓散药理作用的分子机制,为进一步深入揭示其分子作用机制奠定了基础,预测五苓散的潜在作用靶点如PRPS1、HSD17B4等,可为五苓散的深入研究提供新思路。
文摘Over the span of years, improvements over various synthesis methods of graphene are constantly pursued to provide safer and more effective alternatives. Though the extraction of graphene through Hummers method is one of the oldest techniques yet it is one of the most suitable methods for the formation of bulk graphene. Graphene can be obtained in the form of reduced Graphite oxide, sometimes also referred as Graphene oxide. The effectiveness of this oxidation process can be evaluated by the magnitude of carbon/oxygen ratio of the obtained graphene. Here, graphene oxide (GO) was prepared by oxidizing the purified natural flake graphite (NFG) by a modified Hummers method. The attempts have been made to synthesize GO having few layers by using a modified Hummers method where the amount of NaNO3 has been decreased, and the amount of KMnO4 is increased. The reaction has been performed in a 9:1 (by volume) mixture of H2SO4/H3PO4. This modification is successful in increasing the reaction yield and reducing the toxic gas evolution while using a varied proportion of KMnO4 and H2SO4 as those required by Hummers method. A new component of K2S2O8 has been introduced to the reaction system to maintain the pH value. Reduced graphene oxide (rGO) was thereafter extracted by thermal modification of GO. Here, GO has been used as a precursor for graphene synthesis by thermal reduction processes. The results of FTIR and Raman spectroscopy analysis show that the NFG when oxidized by strong oxidants like KMnO4 and NaNO3, introduced oxygen atoms into the graphite layers and formed bonds like C=O, C-H, COOH and C-O-C with the carbon atoms in the graphite layers. The structure and morphology of both GO and rGO were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy, Raman spectroscopy, Brunauer-Emmett-Teller (BET) surface area analysis and differential scanning calorimetry (
基金supported by the National Natural Science Foundation of China(91326202,21225730,21577032)the Fundamental ResearchFunds forthe Central Universities(JB2015001)
文摘Graphene oxide and Ni-Al layered double hydroxides(GO@LDH) nanocomposites were synthesized via a one-pot hydrothermal process,and characterized by X-ray diffraction(XRD),Fourier transformed infrared spectroscopy(FTIR),scanning electron microscopy(SEM),transmission electron microscopy(TEM),X-ray photoelectron spectroscopy(XPS) and Raman spectroscopy in detail.The exploration of U(VI) sorption on GO@LDH surface was performed as a function of ionic strength,solution pH,contact time,U(VI) initial concentrations and temperature.Results of Langmuir isotherms showed that the sorption capacity of GO@LDH(160 mg/g) was much higher than those of LDH(69 mg/g) and GO(92 mg/g).The formed surface complexes between surface oxygen-containing functional groups of GO@LDH and U(VI) turned out to be the interaction mechanism of U(VI) with GO@LDH.According to the thermodynamic studies results,the sorption interaction was actually a spontaneous and endothermic chemical process.The sorption isotherms were better fitted with the Langmuir model compared with other models,which suggested the interaction was mainly dominated by mono layer coverage.The GO@LDH nanocomposites provide potential applications as adsorbents in the enrichment of radionuclides from wastewater in nuclear waste management and environmental remediation.
基金the National Key Technologies Research and Development Program of China(No.2016YFC0701002)
文摘We prepared the nano-inorganic phase-change ＂alloy＂ materials through the modification of Na2SO4·10H2O using Na2HPO4·12H2O and GO nano-nucleating agent, and further investigated their thermophysical properties such as melting/solidification temperatures and enthalpies via differential scanning calorimetry. When the weight ratio of Na2SO4·10H2O and Na2HPO4·12H2O was 8：2 and the weight ratio of graphene oxide was 0.5% of phase change material, the phase change ＂alloy＂ material showed excellent performances, specifically, the melting temperature and latent heat were found to be 22 ℃ and 190 J/g with a degree of subcooling decreased from 8.6℃ to 2.1℃. In order to extend the application of the phase change ＂alloy＂ material to building energy saving field, it was adsorbed on expanded glass beads under vacuum and further covered with diatomite. When the adsorption rate of EGB（volume） and PCAM（weight） was 2.5：1, the particle size of diatomaceous earth was found to be 3.6μm, while the best packaging result was obtained with the melting temperature and latent heat being 21℃ and 135 J/g, and no leakage was observed.
基金supported by the National Natural Science Foundation of China(21473153 and 21207112)the National Natural Science Foundation of Hebei Province(B2013203108)+2 种基金the Science Foundation for the Excellent Youth Scholars from the Universities and Colleges of Hebei Province(YQ2013026)the Support Program for the Top Young Talents of Hebei Province,the Scientific and Technological Research and Development Program of Qinhuangdao City(201502A006)the Open Foundation of National Key Laboratory of Biochemical Engineering(Institute of Process Engineering,Chinese Academy of Sciences(CAS))
文摘Graphene oxide(GO)-chitosan composite hydrogels were successfully prepared via the self-assembly of chitosan molecules and GO. These as-prepared hydrogels were characterized by different techniques. The morphology of the internal network structure of the nanocomposite hydrogels was investigated. The adsorption capacity results demonstrate that the prepared GObased composite hydrogels can efficiently remove three tested dye molecules, Congo red, methylene blue and Rhodamine B, from wastewater in accordance with the pseudo-second-order model. The dye adsorption capacity of the obtained hydrogels is mainly attributed to the GO sheets, whereas the chitosan molecule was incorporated to facilitate the gelation process of the GO sheets. The present study indicates that the as-prepared composite hydrogels can serve as good adsorbents for wastewater treatment as well as the removal of harmful dyes.
