Fluorescent nanomaterials have attracted much attention,due to their unique luminescent properties and promis-ing applications in biomedical areas.In this study,lignin basedfluorescent nanoparticles(LFNP)with high yiel...Fluorescent nanomaterials have attracted much attention,due to their unique luminescent properties and promis-ing applications in biomedical areas.In this study,lignin basedfluorescent nanoparticles(LFNP)with high yield(up to 32.4%)were prepared from lignin nanoparticles(LNP)by one-pot hydrothermal method with ethylene-diamine(EDA)and citric acid.Morphology and chemical structure of LFNP were investigated by SEM,FT-IR,and zeta potential,and it was found that the structure of LFNP changed with the increase of citric acid addition.LFNP showed the highestfluorescence intensity under UV excitation at wavelengths of 375–385 nm,with emis-sion wavelengths between 454–465 nm,and exhibited strong photoluminescence behavior.Meanwhile,with the increase of citric acid content,the energy gap(ΔE)gradually decreased from 3.87 to 3.14 eV,which corresponds to the gradual enhancement offluorescence performance.LFNP also exhibited excellent antioxidant activity,with DPPH free radical scavenging rate increased from 80.8%for LNP up to 96.7%for LFNP,confirming the great potential of these materials for application in biomedicine and cosmetic health care.展开更多
Fluorescent silica organic-inorganic nanohybrids which combine designable luminescence performance of organic fluorescent dyes and various outstanding advantages of silica nanomaterials have attracted increasing resea...Fluorescent silica organic-inorganic nanohybrids which combine designable luminescence performance of organic fluorescent dyes and various outstanding advantages of silica nanomaterials have attracted increasing research interests in these fascinating areas.Optical transparency and facile functional modification properties of silica material provide great opportunities to integrate desired fluorescent molecules for various frontier luminous applications.However,conventional organic dyes are typically subject to aggregation-caused quenching due to their aggregation in silica matrix,which could be detrimental for their performance in sensing and biomedical applications.The appearance of aggregation-induced emission luminogens(AIEgens)paves a new way for developing highly efficient fluorescent silica nanohybrids(FSNs).FSNs with intensive luminescence could be obtained due to the formation of aggregates and the restricted intramolecular motion of AIEgens in silica inorganic matrix.In this review,the reported fabrication methodologies of various FSNs based on colloidal silica nanoparticles(SNs)and mesoporous SNs including physical entrapment and covalent strategies are summarized.Especially,the AIEgens-functionalized silica hybrid nanomaterials are introduced in detail.Furthermore,chemical sensing,biosensing,and bioimaging applications of resultant FSNs are also discussed.展开更多
The ability of human embryonic stem cells(hESCs)to undergo indefinite self-renewal in vitro and to produce lineages derived from all three embryonic germ layers both in vitro and in vivo makes such cells extremely valu...The ability of human embryonic stem cells(hESCs)to undergo indefinite self-renewal in vitro and to produce lineages derived from all three embryonic germ layers both in vitro and in vivo makes such cells extremely valuable in both clinical and research settings.However,the generation of specialized cell lineages from a mixture of differentiated hESCs remains technically difficult.Tissue specific promoter-driven reporter genes are power-ful tools for tracking cell types of interest in differentiated cell populations.Here,we describe the construction of modular lentivectors containing different tissue-specific promoters(Tα1 ofα-tubulin;aP2 of adipocyte Protein 2;and AFP of alpha fetoprotein)driving expression of humanized Renilla greenfluorescent protein(hrGFP).To this end,we used MultiSite gateway technology and employed the novel vectors to successfully monitor hESC differentiation.We present a versatile method permitting target cells to be traced.Our system will facilitate research in developmental biology,transplantation,and in vivo stem cell tracking.展开更多
Within the past two decades,chromophores,which show aggregation-induced emission(AIE),have gained considerable attention with respect to the development of luminescent liquid crystals.In contrast to common luminogens,...Within the past two decades,chromophores,which show aggregation-induced emission(AIE),have gained considerable attention with respect to the development of luminescent liquid crystals.In contrast to common luminogens,AIE emitters do not suffer from aggregation-caused quenching of the emission in the solid state.In this review,we summarize the recent development in the field of AIE-active liquid crystals and show first model devices,which already prove the application potential of these materials.Currently,three different approaches are followed,to get access to luminescent liquid crystals––namely the synthetic approach yielding luminescent mesogens,the doping approach,and the supramolecular approach,which will be described and discussed in detail in this review.展开更多
Two donor-σ-acceptor molecular systems incorporating tetrathiafulvalene(TTF)and tetraphenylpor-phyrin(TPP)units,TTF-TPP(dyad 1)and TTF-TPP-TTF(triad 2),were synthesized.Both dyad 1 and triad 2 and their synthetic int...Two donor-σ-acceptor molecular systems incorporating tetrathiafulvalene(TTF)and tetraphenylpor-phyrin(TPP)units,TTF-TPP(dyad 1)and TTF-TPP-TTF(triad 2),were synthesized.Both dyad 1 and triad 2 and their synthetic intermediates have been characterized by 1H nuclear magnetic resonance(1H NMR)and mass spectro-graphy(MS).Their ultraviolet and visible spectroscopy(UV-Vis)and cyclic voltammetry(CV)showed negligible intramolecular charge transfer interaction in their ground states.Theirfluorescence intensity was strongly quenched compared with TPP,which implied the photoinduced electron transfer occurred from the TTF unit to the TPP unit in the excited state.On the other hand,theirfluorescence intensity could be modulated by sequential oxidation of the TTF unit using chemical methods,which exhibited their potential application influorescence molecular switch.展开更多
Understanding how summer warming influences the parent and daughter shoot production in a perennial clonal grass is vital for comprehending the response of grassland productivity to global warming.Here,we conducted a s...Understanding how summer warming influences the parent and daughter shoot production in a perennial clonal grass is vital for comprehending the response of grassland productivity to global warming.Here,we conducted a simulated experiment using potted Leymus chinensis,to study the relationship between the photosynthetic activ-ity of parent shoots and the production of daughter shoots under a whole(90 days)summer warming scenario(+3°C).The results showed that the biomass of parents and buds decreased by 25.52%and 33.45%,respectively,under warming conditions.The reduction in parent shoot biomass due to warming directly resulted from decreased leaf area(18.03%),chlorophyll a(18.27%),chlorophyll b(29.21%)content,as well as a reduction in net photosynthetic rate(7.32%)and the maximum quantum efficiency of photosystem II(PSII)photochemistry(4.29%).The decline in daughter shoot biomass was linked to a decrease in daughter shoot number(33.33%)by warming.However,the number of belowground buds increased by 46.43%.The results indicated that long-term summer warming reduces biomass accumulation in parent shoot by increasing both limitation of stoma and non-stoma.Consequently,the parent shoot allocates relatively more biomass to the belowground organs to maintain the survival and growth of buds.Overall,buds,as a potential aboveground population,could remedy for the cur-rent loss of parent shoot density by increasing the number of future daughter shoots if summer warming subsides.展开更多
基金This work was financially supported by National Natural Science Foundation of China(51903106)State Administration of Foreign Experts Affairs(G2021144006L).
文摘Fluorescent nanomaterials have attracted much attention,due to their unique luminescent properties and promis-ing applications in biomedical areas.In this study,lignin basedfluorescent nanoparticles(LFNP)with high yield(up to 32.4%)were prepared from lignin nanoparticles(LNP)by one-pot hydrothermal method with ethylene-diamine(EDA)and citric acid.Morphology and chemical structure of LFNP were investigated by SEM,FT-IR,and zeta potential,and it was found that the structure of LFNP changed with the increase of citric acid addition.LFNP showed the highestfluorescence intensity under UV excitation at wavelengths of 375–385 nm,with emis-sion wavelengths between 454–465 nm,and exhibited strong photoluminescence behavior.Meanwhile,with the increase of citric acid content,the energy gap(ΔE)gradually decreased from 3.87 to 3.14 eV,which corresponds to the gradual enhancement offluorescence performance.LFNP also exhibited excellent antioxidant activity,with DPPH free radical scavenging rate increased from 80.8%for LNP up to 96.7%for LFNP,confirming the great potential of these materials for application in biomedicine and cosmetic health care.
基金National Key Research and Development Program of China,Grant/Award Numbers:2019YFC1604601,2019YFC1604600,2017YFA0206901,2017YFA0206900,2018YFC1602301National Natural Science Foundation of China,Grant/Award Number:22005160+2 种基金the major scientific and technological innovation projects of Shandong Province,Grant/Award Number:2018CXGC1406Natural Science Foundation of Shanghai,Grant/Award Number:18ZR1404700Construction project of Shanghai Key Laboratory of Molecular Imaging,Grant/Award Number:18DZ2260400。
文摘Fluorescent silica organic-inorganic nanohybrids which combine designable luminescence performance of organic fluorescent dyes and various outstanding advantages of silica nanomaterials have attracted increasing research interests in these fascinating areas.Optical transparency and facile functional modification properties of silica material provide great opportunities to integrate desired fluorescent molecules for various frontier luminous applications.However,conventional organic dyes are typically subject to aggregation-caused quenching due to their aggregation in silica matrix,which could be detrimental for their performance in sensing and biomedical applications.The appearance of aggregation-induced emission luminogens(AIEgens)paves a new way for developing highly efficient fluorescent silica nanohybrids(FSNs).FSNs with intensive luminescence could be obtained due to the formation of aggregates and the restricted intramolecular motion of AIEgens in silica inorganic matrix.In this review,the reported fabrication methodologies of various FSNs based on colloidal silica nanoparticles(SNs)and mesoporous SNs including physical entrapment and covalent strategies are summarized.Especially,the AIEgens-functionalized silica hybrid nanomaterials are introduced in detail.Furthermore,chemical sensing,biosensing,and bioimaging applications of resultant FSNs are also discussed.
文摘The ability of human embryonic stem cells(hESCs)to undergo indefinite self-renewal in vitro and to produce lineages derived from all three embryonic germ layers both in vitro and in vivo makes such cells extremely valuable in both clinical and research settings.However,the generation of specialized cell lineages from a mixture of differentiated hESCs remains technically difficult.Tissue specific promoter-driven reporter genes are power-ful tools for tracking cell types of interest in differentiated cell populations.Here,we describe the construction of modular lentivectors containing different tissue-specific promoters(Tα1 ofα-tubulin;aP2 of adipocyte Protein 2;and AFP of alpha fetoprotein)driving expression of humanized Renilla greenfluorescent protein(hrGFP).To this end,we used MultiSite gateway technology and employed the novel vectors to successfully monitor hESC differentiation.We present a versatile method permitting target cells to be traced.Our system will facilitate research in developmental biology,transplantation,and in vivo stem cell tracking.
基金Prof.Werdelmann StiftungBoehringer Ingelheim Stiftung+1 种基金Center for Nanointegration Duisburg-EssenFonds der chemischen Industrie。
文摘Within the past two decades,chromophores,which show aggregation-induced emission(AIE),have gained considerable attention with respect to the development of luminescent liquid crystals.In contrast to common luminogens,AIE emitters do not suffer from aggregation-caused quenching of the emission in the solid state.In this review,we summarize the recent development in the field of AIE-active liquid crystals and show first model devices,which already prove the application potential of these materials.Currently,three different approaches are followed,to get access to luminescent liquid crystals––namely the synthetic approach yielding luminescent mesogens,the doping approach,and the supramolecular approach,which will be described and discussed in detail in this review.
基金supported by the National Natural Science Foundation of China(Grant Nos.21076078 and 20676036).
文摘Two donor-σ-acceptor molecular systems incorporating tetrathiafulvalene(TTF)and tetraphenylpor-phyrin(TPP)units,TTF-TPP(dyad 1)and TTF-TPP-TTF(triad 2),were synthesized.Both dyad 1 and triad 2 and their synthetic intermediates have been characterized by 1H nuclear magnetic resonance(1H NMR)and mass spectro-graphy(MS).Their ultraviolet and visible spectroscopy(UV-Vis)and cyclic voltammetry(CV)showed negligible intramolecular charge transfer interaction in their ground states.Theirfluorescence intensity was strongly quenched compared with TPP,which implied the photoinduced electron transfer occurred from the TTF unit to the TPP unit in the excited state.On the other hand,theirfluorescence intensity could be modulated by sequential oxidation of the TTF unit using chemical methods,which exhibited their potential application influorescence molecular switch.
基金funded by the NSFC(32371669)the Science and Technology Talent Project for Distinguished Young Scholars of Jilin Province(20240602009RC)+1 种基金the NSF of Jilin Province(20240101207JC)the Scientific Research Project of the Department of Education,Jilin Province(JJKH20230687KJ).
文摘Understanding how summer warming influences the parent and daughter shoot production in a perennial clonal grass is vital for comprehending the response of grassland productivity to global warming.Here,we conducted a simulated experiment using potted Leymus chinensis,to study the relationship between the photosynthetic activ-ity of parent shoots and the production of daughter shoots under a whole(90 days)summer warming scenario(+3°C).The results showed that the biomass of parents and buds decreased by 25.52%and 33.45%,respectively,under warming conditions.The reduction in parent shoot biomass due to warming directly resulted from decreased leaf area(18.03%),chlorophyll a(18.27%),chlorophyll b(29.21%)content,as well as a reduction in net photosynthetic rate(7.32%)and the maximum quantum efficiency of photosystem II(PSII)photochemistry(4.29%).The decline in daughter shoot biomass was linked to a decrease in daughter shoot number(33.33%)by warming.However,the number of belowground buds increased by 46.43%.The results indicated that long-term summer warming reduces biomass accumulation in parent shoot by increasing both limitation of stoma and non-stoma.Consequently,the parent shoot allocates relatively more biomass to the belowground organs to maintain the survival and growth of buds.Overall,buds,as a potential aboveground population,could remedy for the cur-rent loss of parent shoot density by increasing the number of future daughter shoots if summer warming subsides.
