弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)是恶性淋巴瘤REAL(revised European-American lymphoma)分类和WHO(world health organization)分类中最常见的非霍奇金淋巴瘤(non-hodgkin lym-phoma,NHL)类型,DLBCL是一种异...弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)是恶性淋巴瘤REAL(revised European-American lymphoma)分类和WHO(world health organization)分类中最常见的非霍奇金淋巴瘤(non-hodgkin lym-phoma,NHL)类型,DLBCL是一种异质性很强的病症,发病机制错综复杂,涉及染色体易位,其中bcl-6基因的3q27染色体易位较为常见,还涉及bcl-2基因t(14;18)(q32;q21)的易位,以及t(8;14)(q24;q32)与IgH基因融合所发生的病变;异常体细胞高频突变涉及pim-1、myc、RhoH/TTF和PAX5等原癌基因;p53基因失活与其他基因突变;p16基因的沉默表达;原癌基因rel、myc和bcl-2扩增等多个方面,从以上方面总结不同基因病变在DLBCL发病中的作用.展开更多
ObjectiveTo investigate BCL 6 gene mutations in Chinese populations with B cell non Hodgkin's lymphoma Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct D...ObjectiveTo investigate BCL 6 gene mutations in Chinese populations with B cell non Hodgkin's lymphoma Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5' noncoding region of the BCL 6 gene in a total of 40 cases of diffuse large cell lymphoma (DLCL) and follicular lymphoma (FL) Results Nine cases were found to have base substitutions The incidence of BCL 6 gene mutation and the frequency of single base changes were approximately 25 7% and (0 56-1 10)×10 2 /bp, respectively Conclusions The 5' regulatory region of the BCL 6 gene undergoes frequent somatic hypermutation during lymphomagenesis and the identification of BCL 6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B NHL展开更多
文摘弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)是恶性淋巴瘤REAL(revised European-American lymphoma)分类和WHO(world health organization)分类中最常见的非霍奇金淋巴瘤(non-hodgkin lym-phoma,NHL)类型,DLBCL是一种异质性很强的病症,发病机制错综复杂,涉及染色体易位,其中bcl-6基因的3q27染色体易位较为常见,还涉及bcl-2基因t(14;18)(q32;q21)的易位,以及t(8;14)(q24;q32)与IgH基因融合所发生的病变;异常体细胞高频突变涉及pim-1、myc、RhoH/TTF和PAX5等原癌基因;p53基因失活与其他基因突变;p16基因的沉默表达;原癌基因rel、myc和bcl-2扩增等多个方面,从以上方面总结不同基因病变在DLBCL发病中的作用.
基金ThisworkwassupportedbythegrantsfromtheNationalNaturalScienceFoundationofChina (No 3 9770 83 0 ) andDr ClydeFoundation
文摘ObjectiveTo investigate BCL 6 gene mutations in Chinese populations with B cell non Hodgkin's lymphoma Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5' noncoding region of the BCL 6 gene in a total of 40 cases of diffuse large cell lymphoma (DLCL) and follicular lymphoma (FL) Results Nine cases were found to have base substitutions The incidence of BCL 6 gene mutation and the frequency of single base changes were approximately 25 7% and (0 56-1 10)×10 2 /bp, respectively Conclusions The 5' regulatory region of the BCL 6 gene undergoes frequent somatic hypermutation during lymphomagenesis and the identification of BCL 6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B NHL
