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骨髓间充质干细胞研究进展 被引量:25
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作者 王庆贤 张英泽 +1 位作者 马哲 韩长玲 《中华实验外科杂志》 CAS CSCD 北大核心 2003年第6期575-576,共2页
关键词 骨髓干细胞 研究进展 生理功能 免疫调节
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Intravenous injection of mesenchymal stem cells is effective in treating liver fibrosis 被引量:32
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作者 Wei Zhao Jun-Jie Li +6 位作者 Da-Yong Cao Xiao Li Lin-Ying Zhang Yong He Shu-Qiang Yue De-Sheng Wang Ke-Feng Dou 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第10期1048-1058,共11页
AIM: To compare the influence of different transplant sites in bone marrow mesenchymal stem cell (MSC)-based therapy for liver fibrosis. METHODS: MSCs isolated from Sprague Dawley (SD) rats were induced into hepatocyt... AIM: To compare the influence of different transplant sites in bone marrow mesenchymal stem cell (MSC)-based therapy for liver fibrosis. METHODS: MSCs isolated from Sprague Dawley (SD) rats were induced into hepatocyte-like cells. Liver fibrosis in SD rats was induced with carbon tetrachloride. Following hepatocyte induction in vitro, 4',6-diamidino- 2-phenylindole (DAPI)-labeled MSCs were transplanted by intravenous, intrahepatic, and intraperitoneal injection. Histopathological staining, immunohistochemistry, and biochemical analysis were used to compare the morphological and functional liver regeneration among different MSC injection modalities. The expression differences of interleukins, growth factor, extracellular matrix, matrix metalloproteinases, and tissue inhibitor of metalloproteinase were examined by real-time reverse transcription-polymerase chain reaction (RT-PCR) andenzyme linked immunosorbent assay (ELISA). RESULTS: Four days after exposure to hepatocyte differentiation medium, MSCs that did not express hepatocyte markers could express α-fetoprotein, albumin, and cytokeratin 18. The results of histopathological staining, immunohistochemistry, and biochemical analysis indicated that intravenous injection is more effective at rescuing liver failure than other injection modalities. DAPI-labeled cells were found around liver lobules in all three injection site groups, but the intravenous group had the highest number of cells. PCR and ELISA analysis indicated that interleukin-10 (IL-10) was highest in the intravenous group, whereas il1β, il6, tnfα and tgfβ, which can be regulated by IL10 and are promoters of liver fibrosis, were significantly lower than in the other groups. CONCLUSION: MSC administration is able to protect against liver fibrosis. Intravenous injection is the most favorable treatment modality through promotion of IL10 expression. 展开更多
关键词 Mesenchymal stem cells Hepatocyte differentiation Intravenous injection Liver fibrosis INTERLEUKIN-10
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Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction 被引量:11
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作者 WANG Jian-an LUO Rong-hua +5 位作者 ZHANG Xing XIE Xiao-jie HU Xin-yang HE Ai-na CHEN Jie LI Jia-hui 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2006年第8期641-647,共7页
Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme i... Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors (ACEIs) perindopril into ischemic myocardium after acute myocardial infarction. Methods: Forty rats were divided into four groups: control, MSC, ACEI, MSC+ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC+ACEI group. Phosphate-buffered saline (PBS) was injected into control group. Perindopril was administered p.o. to ACEI group and MSC+ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson’s trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase (MMP)-2, matrix metalloproteinase (MMP)-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 in infarction area was detected by reverse transcriptase PCR (RT-PCR) analysis. Results: The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group, ACEI group, and MSC+ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4 groups. Conclusion: Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism. 展开更多
关键词 REMODELLING Acute myocardial infarction PERINDOPRIL Bone marrow mesenchymal stem cell
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骨髓间充质干细胞与成骨 被引量:9
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作者 叶成 史鹏 《中国矫形外科杂志》 CAS CSCD 北大核心 2008年第5期356-358,共3页
关键词 成骨细胞表型 骨髓干细胞 骨种子细胞 骨组织工程学 原始细胞 机体损伤 体外培养 自我更新
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Role of bone marrow-derived mesenchymal stem cells in a rat model of severe acute pancreatitis 被引量:13
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作者 Xiao-Huang Tu Jing-Xiang Song +5 位作者 Xiao-Jun Xue Xian-Wei Guo Yun-Xia Ma Zhi-Yao Chen Zhong-Dong Zou Lie Wang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第18期2270-2279,共10页
AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into thr... AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into three groups:nonsodium deoxycholate(SDOC) group(non-SODC group),SDOC group,and a MSCs intervention group(i.e.,a co-culture system of MSCs and pancreatic acinar cells + SDOC).The cell survival rate,the concentration of malonaldehyde(MDA),the density of superoxide dismutase(SOD),serum amylase(AMS) secretion rate and lactate dehydrogenase(LDH) leakage rate were detected at various time points.In a separate study,Sprague Dawley rats were randomly divided into either an SAP group or an SAP + MSCs group.Serum AMS,MDA and SOD,interleukin(IL)-6,IL-10,and tumor necrosis factor(TNF)-α levels,intestinal mucosa injury scores and proliferating cells of small intestinal mucosa were measured at various time points after injecting either MSCs or saline into rats.In both studies,the protective effect of MSCs was evaluated.RESULTS:In vitro,The cell survival rate of pancreatic acinar cells and the density of SOD were significantly reduced,and the concentration of MDA,AMS secretion rate and LDH leakage rate were significantly increased in the SDOC group compared with the MSCs intervention group and the Non-SDOC group at each time point.In vivo,Serum AMS,IL-6,TNF-α and MAD level in the SAP + MSCs group were lower than the SAP group;however serum IL-10 level was higher than the SAP group.Serum SOD level was higher than the SAP group at each time point,whereas a significant betweengroup difference in SOD level was only noted after 24 h.Intestinal mucosa injury scores was significantly reduced and the proliferating cells of small intestinal mucosa became obvious after injecting MSCs.CONCLUSION:MSCs can effectively relieve injury to pancreatic acinar cells and small intestinal epithelium,promote the proliferation of enteric epithelium and repair of the mucosa,attenuate systemic inflammation in rats with SAP. 展开更多
关键词 Bone marrow mesenchymal stem cells Severe acute pancreatitis Intestinal barricade function Pancreatic acinar cells
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Ectopic bone formation of human bone morphogenetic protein-2 gene transfected goat bone marrow-derived mesenchymal stem cells in nude mice 被引量:12
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作者 汤亭亭 徐小良 +3 位作者 戴尅戎 郁朝锋 岳冰 楼觉人 《Chinese Journal of Traumatology》 CAS 2005年第1期3-7,共5页
Objective: To evaluate the osteogenic potential of bone morphogenetic protein (BMP)-2 gene transfected goat bone marrow-derived mesenchymal stem cells (MSCs). Methods: Goat bone marrow- derived MSCs were transfected b... Objective: To evaluate the osteogenic potential of bone morphogenetic protein (BMP)-2 gene transfected goat bone marrow-derived mesenchymal stem cells (MSCs). Methods: Goat bone marrow- derived MSCs were transfected by Adv-human bone morphogenetic protein (hBMP)-2 gene(Group 1), Adv-beta gal transfected MSCs (Group 2)and uninfected MSCs(Group 3). Western blot analysis, alkaline phosphatase staining, Von Kossa staining and transmission electron microscopy were adopted to determine the phenotype of MSCs. Then the cells were injected into thigh muscles of the nude mice. Radiographical and histological evaluations were performed at different intervals. Results: Only Adv-hBMP-2 transfected MSCs produced hBMP-2. These cells were positive for alkaline phosphatase staining at the 12th day and were positive for Von Kossa staining at the 16th day after gene transfer. Electron microscopic observation showed that there were more rough endoplasmic reticulum, mitochondria and lysosomes in Adv-hBMP-2 transfected MSCs compared to MSCs of other two groups. At the 3rd and 6th weeks after cell injection, ectopic bones were observed in muscles of nude mice of Group 1. Only fibrous tissue or a little bone was found in other two groups. Conclusions: BMP-2 gene transfected MSCs can differentiate into osteoblasts in vitro and induce bone formation in vivo. 展开更多
关键词 Bone morphogenetic proteins Gene therapy Mesenchymal stem cells Mice nude
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Effect of kidney-reinforcing,blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesenchymal stem cells from chronic aplastic anemia patients 被引量:12
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作者 Baodong Ye Dan Chen +6 位作者 Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qinhong Yu 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2012年第4期596-603,共8页
OBJECTIVE: To explore the effect of kidney-rein- forcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone mar- row mesenchymal stem cells (MSCs) from patients with chronic aplas... OBJECTIVE: To explore the effect of kidney-rein- forcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone mar- row mesenchymal stem cells (MSCs) from patients with chronic aplastic anemia (CAA). METHODS: We used three Traditional Chinese Medicine recipes, namely a kidney-reinforcing recipe (KRR), blood-activating and stasis-removing recipe (BASRR), and kidney-reinforcing, blood-activating and stasis-removing recipe (KRBASFIR), and a nor- mal saline control to prepare herbal medicine se- rum in Sprague Dawley rats. Thirty CAA patients were enrolled in the experimental group, including 17 kidney-Yang deficient patients and 13 kidney-Yin deficient patients. Ten healthy individuals were included in the control group. MSCs were isolated from bone marrow samples, and the cell density was observed to measure their proliferation ability by microscopy on days 2, 7, and 14 after isolation. In addition, the expression of adhesion molecules of bone marrow MSCs (CD106, CD49d, CD31 and CD44) were detected by flow cytometry after 48 h of treatment with the four different herbal medi- cine serums. RESULTS: The proliferation of MSCs from kid- ney-Yang deficient and kidney-Yin deficient pa- tients was weaker than that of MSCs from the con- trol group. The expression of all adhesion mole- cules of bone marrow MSCs from CAA patients was obviously lower than that in the control group (P〈 0.01). The expression of CD49d and CD31 in MSCs from patients with a kidney-Yin deficiency was low- er than in those with a kidney-yang deficiency (P〈 0.05 and P〈O.01, respectively). For kidney-Yang defi- cient patients, CD31 expression in the KRBASRR group was significantly higher than that in the BASRR group (P〈O.01), while CD44 in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈O.01). For kidney-Yin defi- cient patients, CD106 and CD49d expression in the KRBASRR group was obviously higher than that in the KRR group (P 展开更多
关键词 Anemia Aplastic Bone marrow Mesen-chymal stem cells Cell adhesion molecules Rein-forcing kidney Blood-activating stasis-removing
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Effects of mesenchymal stem cells transfected with human hepatocyte growth factor gene on healing of burn wounds 被引量:9
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作者 哈小琴 吕同德 +1 位作者 惠玲 董芳 《Chinese Journal of Traumatology》 CAS 2010年第6期349-355,共7页
Objective: To explore the effects of bone marrow-derived mesenchymal stem cells (BMSCs)transfected with adenoviral vector carrying hepatocyte growth factor (HGF, Ad-HGF) on burn wound healing.Methods: BMSCs from... Objective: To explore the effects of bone marrow-derived mesenchymal stem cells (BMSCs)transfected with adenoviral vector carrying hepatocyte growth factor (HGF, Ad-HGF) on burn wound healing.Methods: BMSCs from male Wistar rats were separated and purified with Percoll separating medium by density gradient centrifugation and cultured with DMEM containing 20% fetal bovine serum (FBS). Then BMSCs were transfected with Ad-HGF at the optimal gene transduction efficiency of 100 multiplicity of infection (MOI). The efficiency of transfection and the expression of HGF in the suspension were detected by flow cytometry and enzyme linked immunosorbent assay (ELISA) respectively. Thirtytwo female rats were subjected to 90℃ water for 12 seconds to induce a partial thickness skin burn. The animals were randomly divided into mesenchymal stem cells (MSCs) treatment group (Group A), Ad-HGF treatment group (Group B),Ad-HGF-modified MSCs treatment group (Group C) and saline control group (Group D). On days 3, 5, 7, 14 and 21 postburn, HE and Sirius red stain were performed to observe the burn wound healing and collagen content. The content of hydroxyproline in wounds was also detected.Transplanted cells and the expression of(sex-determining region Y) SRY gene were detected by in situ hybridization and polymerase chain reaction (PCR), while the expression of HGF in wound tissues was detected by ELISA.Results: The result of flow cytometry showed that the transfection efficiency was 86.41% at 100 MOI. Compared with the control group, the content of HGF in the supernatant after transfection increased time-dependently and peaked at 48 h, showing significant differences at 24 h, 48 h,72 h and 96 h (P<0.01 ). Results of HE stain revealed that the range of re-epidermidalization in Group C was significantly larger than that in other groups in the first week. Three weeks postburn, the epidermis was significantly thicker in Group C than in other groups and the nails of dermis inse 展开更多
关键词 HGF protein human Mesenchymal stem cells TRANSFECTION BURNS Wound healing
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Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging 被引量:9
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作者 REN ZhenHua WANG JiaYin +2 位作者 ZOU ChunLin GUAN YunQian ZHANG Yu Alex 《Science China(Life Sciences)》 SCIE CAS 2011年第11期981-987,共7页
Recently,transplantation of allogeneic and autologous cells has been used for regenerative medicine.A critical issue is monitoring migration and homing of transplanted cells,as well as engraftment efficiency and funct... Recently,transplantation of allogeneic and autologous cells has been used for regenerative medicine.A critical issue is monitoring migration and homing of transplanted cells,as well as engraftment efficiency and functional capability in vivo.Monitoring of superparamagnetic iron oxide(SPIO) particles by magnetic resonance imaging(MRI) has been used in animal models and clinical settings to track labeled cells.A major limitation of MRI is that the signals do not show biological characteristics of transplanted cells in vivo.Bone marrow mesenchymal stem cells(MSCs) have been extensively investigated for their various therapeutic properties,and exhibit the potential to differentiate into cells of diverse lineages.In this study,cynomolgus monkey MSCs(cMSCs) were labeled with Molday ION Rhodamine-BTM(MIRB),a new SPIO agent,to investigate and characterize the biophysical and MRI properties of labeled cMSCs in vitro and in vivo.The results indicate that MIRB is biocompatible and useful for cMSCs labeling and cell tracking by multimodality imaging.Our method is helpful for detection of transplanted stem cells in vivo,which is required for understanding mechanisms of cell therapy. 展开更多
关键词 bone marrow mesenchymal stem cells MRI Molday ION Rhodamine-BTM TRANSPLANTATION nonhuman primate
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Comparison of human amniotic fluid-derived and umbilical cord Wharton's Jelly-derived mesenchymal stromal cells: Characterization and myocardial differentiation capacity 被引量:9
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作者 Jing Bai Yuan Hu +4 位作者 Yi-Ru Wang Li-Feng Liu Jie Chen Shao-Ping Su Yu Wang 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2012年第2期166-171,共6页
Objective To compare the characterization and myocardial differentiation capacity of arnniotic fluid-derived mesenchymal stromal cells (AF MSCs) and umbilical cord Wharton's Jelly-derived mesenchymal stromal cells ... Objective To compare the characterization and myocardial differentiation capacity of arnniotic fluid-derived mesenchymal stromal cells (AF MSCs) and umbilical cord Wharton's Jelly-derived mesenchymal stromal cells (WJ MSCs). Methods The human AF MSCs were cultured from amniotic fluid samples obtained by amniocentesis. The umbilical cord WJ MSCs were obtained from Wharton's Jelly of umbilical cords of infants delivered full-term by normal labor. The morphology, growth curves, and analyses by flow cytometry of cell surface markers were compared between the two types of cells. Myocardial genes (GATA-4, c-TnT, a-actin, and Cx43) were detected by real-time PCR and the corresponding protein expressions were detected by Western blot analysis after myocardial induced in AF MSCs and WJ MSCs. Results Our findings revealed AF MSCs and WJ MSCs shared similar morphological characteristics of the fibroblastoid shape. The AF MSCs were easily obtained than the WJ MSCs and had a shorter time to reach adherence of 2.7 ± 1.6 days to WJ MSCs of 6.5 ± 1.8 days. The growth curves by MTT cytotoxic assay showed the AF MSCs had a similar proliferative capacity at passage 5 and passage 10. However, the proliferative capacities ofWJ MSCs were decreased at 5 passage relative to 10 passage. Both AF stem cells and WJ stem cells had the characteristics of mesenchymal stromal cells with some characteristics of embryonic stem cells. They express CD29 and CD105, but not CD34. They were positive for Class I major histocompatibility (MHC I) antigens (HLA-ABC), and were negative, or mildly positive, for MHC Class II (HLA-DR) antigen. Oct-4 was positive in all the two cells types. Both AF MSCs and WJ MSCs could differentiate along myocardium. The differentiation capacities were detected by the expression of GATA-4, c-TnT, a-actin, Cx43 after myocardial induction. Conclusions Both AF MSCs and WJ MSCs have the potential clinical application for myogenesis in cardiac regenerative therapy. 展开更多
关键词 Human amniotic fluid-derived ceUs Wharton's Jelly-derived cells Myocardial differentiation Mesenchymal stromal cells
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Increased leptin by hypoxic-preconditioning promotes autophagy of mesenchymal stem cells and protects them from apoptosis 被引量:8
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作者 WANG LiHan HU XinYang +4 位作者 ZHU Wei JIANG Zhi ZHOU Yu CHEN PanPan WANG JianAn 《Science China(Life Sciences)》 SCIE CAS 2014年第2期171-180,共10页
Autophagy is the basic catabolic progress involved in cell degradation of unnecessary or dysfunctional cellular components.It has been proven that autophagy could be utilized for cell survival under stresses.Hypoxic-p... Autophagy is the basic catabolic progress involved in cell degradation of unnecessary or dysfunctional cellular components.It has been proven that autophagy could be utilized for cell survival under stresses.Hypoxic-preconditioning(HPC)could reduce apoptosis induced by ischemia and hypoxia/serum deprivation(H/SD)in bone marrow-derived mesenchymal stem cells(BMSCs).Previous studies have shown that both leptin signaling and autophagy activation were involved in the protection against apoptosis induced by various stress,including ischemia-reperfusion.However,it has never been fully understood how leptin was involved in the protective effects conferred by autophagy.In the present study,we demonstrated that HPC can induce autophagy in BMSCs by increased LC3-II/LC3-I ratio and autophagosome formation.Interestingly,similar effects were also observed when BMSCs were pretreated with rapamycin.The beneficial effects offered by HPC were absent when BMSCs were incubated with autophagy inhibitor,3-methyladenine(3-MA).In addition,down-regulated leptin expression by leptin-shRNA also attenuated HPC-induced autophagy in BMSCs,which in turn was associated with increased apoptosis after exposed to sustained H/SD.Furthermore,increased AMP-activated protein kinase phosphorylation and decreased mammalian target of rapamycin phosphorylation that were observed in HPC-treated BMSCs can also be attenuated by down-regulation of leptin expression.Our data suggests that leptin has impact on HPC-induced autophagy in BMSCs which confers protection against apoptosis under H/SD,possibly through modulating both AMPK and mTOR pathway. 展开更多
关键词 BMSCs AUTOPHAGY hypoxic-preconditioning LEPTIN APOPTOSIS
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Combinatorial effects of NaomaiYihao Capsules(脑脉一号胶囊) and vascular endothelial growth factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in cerebral ischemic tis sues in rats 被引量:8
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作者 郭建文 陈朝 +1 位作者 黄燕 黎奔 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2012年第1期87-92,共6页
OBJECTIVE:To investigate the combinatorial effects of Naomai Yihao(NMYH) Capsules(脑脉一号胶囊) and vascular endothelial growth factor(VEGF) gene-transfected bone marrow mesenchymal stem cells(BMSCs) on angiogenesis i... OBJECTIVE:To investigate the combinatorial effects of Naomai Yihao(NMYH) Capsules(脑脉一号胶囊) and vascular endothelial growth factor(VEGF) gene-transfected bone marrow mesenchymal stem cells(BMSCs) on angiogenesis in cerebral ischemic tissues in rats and the mechanism.METHOD:BMSCs were isolated and cultured from bone marrow by an adherence method.Then,BMSCs were transfected with the eukaryotic expression plasmid pEGFP-VEGF 165 by positive ionic liposome transfection.A rat model of middle cerebral artery occlusion(MCAO) was established.Rats were allocated to six groups:model,BMSC,VEGF gene-transfected BMSC transplantation(BMSC/VEGF),NMYH,combined NMYH and BMSC/VEGF(combined treatment group) and sham operation groups.The behavioral rating score(BRS) of rat and the expression of CD34 and VEGF in brain tis sue were measured by immunohistochemistry on days 7,14 and 21 after reperfusion.Angiogenesi was observed and evaluated with laser scanning confocal microscopy.RESULTS:The BRS of rats in NMYH,BMSC transplan tation and combined treatment groups was significantly lower than that of the model group(P< 0.001),with no significant difference between NMYH and transplantation groups(P=0.619).The expression of CD34 andVEGF in NMYH,transplanta tion and combined treatment groups increased(P< 0.001),with a significant difference between NMYH and transplantation groups(P<0.001).The blood vessel area in NMYH,transplantation and com bined treatment groups was significantly increased(P<0.05),without a significant difference between NMYH and transplantation groups(P=0.873).CONCLUSIONS:VEGF gene-transfected BMSCs im prove angiogenesis in the cerebral ischemic area NMYH Capsules promote angiogenesis in MCAO rats treated with BMSC transplantation,which show an improved BRS.The mechanism of angio genesis may be related to up-regulation ofVEGF ex pression. 展开更多
关键词 Bone marrow mesenchymal stem cells naomai yihao capsule Vascular endothelial growth factor Eukaryotic expression plasmid Transplantation Cerebral ischemia ANGIOGENESIS
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大鼠骨髓间充质干细胞向肝细胞诱导分化研究 被引量:7
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作者 王宾 程云霄 +2 位作者 吕云霄 沈来根 刘震杰 《中华实验外科杂志》 CAS CSCD 北大核心 2013年第1期169-170,共2页
我们以干细胞生长因子(HGF)和α-成纤维细胞生长因子(α-FGF)诱导大鼠骨髓间充质干细胞(BMSCs)特定向肝细胞分化,为进一步通过干细胞移植治疗肝脏疾病提供依据。
关键词 骨髓干细胞 细胞分化 诱导大鼠 细胞诱导分化 成纤维细胞生长因子 干细胞生长因子 肝脏疾病 移植治疗
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Timing of transplantation of autologous bone marrow derived mesenchymal stem cells for treating myocardial infarction 被引量:7
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作者 CHEN YiHuan TENG XiaoMei +4 位作者 CHEN WeiQian YANG JunJie YANG ZiYing YU YunSheng SHEN ZhenYa 《Science China(Life Sciences)》 SCIE CAS 2014年第2期195-200,共6页
It is still unclear whether the timing of intracoronary stem cell therapy affects the therapeutic response in patients with myocardial infarction.The natural course of healing the infarction and the presence of putati... It is still unclear whether the timing of intracoronary stem cell therapy affects the therapeutic response in patients with myocardial infarction.The natural course of healing the infarction and the presence of putative homing signals within the damaged myocardium appear to favor cell engraftment during the transendothelial passage in the early days after reperfusion.However,the adverse inflammatory environment,with its high oxidative stress,might be deleterious if cells are administered too early after reperfusion.Here we highlight several aspects of the timing of intracoronary stem cell therapy.Our results showed that transplantation of bone marrow mesenchymal stem cells at 2 4 weeks after myocardial infarction is more favorable for reduction of the scar area,inhibition of left ventricular remodeling,and recovery of heart function.Coronary injection of autologous bone marrow mesenchymal stem cells at 2 4 weeks after acute myocardial infarction is safe and does not increase the incidence of complications. 展开更多
关键词 bone marrow mesenchymal stem cells(BMSCs) cell transplantation myocardial infarction
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中药单味或单体诱导骨髓间充质干细胞向成骨细胞分化研究概况 被引量:7
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作者 谢兴文 徐世红 李宁 《中国中医骨伤科杂志》 CAS 2010年第10期65-67,共3页
关键词 中药 骨髓干细胞 成骨细胞 分化
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骨髓间充质干细胞移植促进糖尿病大鼠β细胞再生的机制 被引量:5
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作者 程愈 申晶 +4 位作者 郝好杰 李冰 郭恩凯 韩为东 母义明 《解放军医学院学报》 CAS 2013年第6期610-613,共4页
目的探索骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)移植促进糖尿病大鼠模型中β细胞再生的机制。方法正常SD大鼠给予单次大剂量链脲佐菌素(streptozotocin,STZ)(65 mg/kg)注射,3 d后开始监测血糖,连续3 d随机血糖>... 目的探索骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)移植促进糖尿病大鼠模型中β细胞再生的机制。方法正常SD大鼠给予单次大剂量链脲佐菌素(streptozotocin,STZ)(65 mg/kg)注射,3 d后开始监测血糖,连续3 d随机血糖>28 mmol/L者选为实验对象。所有实验对象随机分为对照组(n=15),BMSC移植组(n=15),BMSC培养上清移植组(n=15)以及BMSC示踪组(以Dil染料标记BMSCs,n=5),分别接受PBS、MSC、MSC培养上清及Dil-BMSC的移植。结果 BMSC移植后30 d左右血糖水平有明显改善[(20.4±0.98)mmol/L,n=15],胰岛素水平有明显提高[(0.99±0.23)ng/ml,n=15],β细胞质量明显增长。富含BMSC分泌因子的BMSC上清输注后,糖尿病大鼠血糖有一定程度的降低[(26.2±1.53)mmol/L,n=15],胰岛素水平有所恢复[(0.341±0.08)ng/ml,n=15],β细胞质量较对照组有所增加。在胰腺仅检测到小部分BMSC,这部分归巢于胰腺的BMSC中检测不到与胰岛素的共标。结论 BMSC的分泌效应为BMSC促进糖尿病动物模型中β细胞再生的机制。 展开更多
关键词 骨髓干细胞 骨髓干细胞 骨髓干细胞培养上清 糖尿病 β细胞再生
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骨髓间充质干细胞分离培养和应用 被引量:4
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作者 周德存 严中亚 《安徽医学》 2003年第4期78-79,共2页
关键词 骨髓干细胞 分离 培养 临床应用 免疫学基础
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microRNA-21a-5p/PDCD4 axis regulates mesenchymal stem cell-induced neuroprotection in acute glaucoma 被引量:6
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作者 Wenru Su Zuohong Li +6 位作者 Yu Jia Yingting Zhu Wenjia Cai Peixing Wan Yingying Zhang Song Guo Zheng Yehong Zhuo 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2017年第4期289-301,共13页
Mesenchymal stem ceils (MSCs) have been demonstrated to have promising therapeutic benefits for a variety of neurological dis- eases; however, the underlying mechanisms are poorly understood. Here, we showed that in... Mesenchymal stem ceils (MSCs) have been demonstrated to have promising therapeutic benefits for a variety of neurological dis- eases; however, the underlying mechanisms are poorly understood. Here, we showed that intravitreal infusion of MSCs promoted retinal ganglion cell (RGC) survival in a mouse model of acute glaucoma, with significant inhibition of microglial activation, production of TNF-α, IL-1β, and reactive oxygen species, as well as caspase-8 and caspase-3 activation. In vitro, MSCs inhibited both caspase-8-mediated RGC apoptosis and microgUal activation, partly via the action of stanniocalcin 1 (STCl). Furthermore, we found that microRNA-21a-Sp (miR-21) and its target, PDCD4, were essential for STC1 production and the neuroprotective property of MSCs in vitro and in vivo. Importantly, miR-21 overexpression or PDCD4 knockdown augmented MSC-mediated neuroprotective effects on acute glaucoma. These data highlight a previously unrecognized neuroprotective mechanism by which the miR-21/ PDCD4 axis induces MSCs to secrete STC1 and other factors that exert neuroprotective effects. Therefore, modulating the miR- 21/PDCD4 axis might be a promising strategy for clinical treatment of acute glaucoma and other neurological diseases. 展开更多
关键词 mesenchymal stem ceils microRNA NEUROPROTECTION GLAUCOMA neurological diseases
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髓芯减压术联合自体骨髓间充质干细胞移植治疗早期股骨头坏死11例报道 被引量:2
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作者 诸葛福煜 《菏泽医学专科学校学报》 2023年第2期79-79,91,共2页
2016年1月—2019年12月我院采用髓芯减压术联合自体骨髓间充质干细胞移植治疗早期股骨头坏死患者11例,男7例,女4例;年龄43~75岁,平均年龄62.27岁;左侧6例,右侧5例;Ficat and Arlet分期Ⅰ期4例、Ⅱ期7例。治疗方法:全身麻醉或硬膜外麻醉... 2016年1月—2019年12月我院采用髓芯减压术联合自体骨髓间充质干细胞移植治疗早期股骨头坏死患者11例,男7例,女4例;年龄43~75岁,平均年龄62.27岁;左侧6例,右侧5例;Ficat and Arlet分期Ⅰ期4例、Ⅱ期7例。治疗方法:全身麻醉或硬膜外麻醉,患者仰卧位,常规术区皮肤消毒铺无菌巾单。 展开更多
关键词 股骨头坏死 髓芯减压术 骨髓干细胞
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骨髓间充质干细胞与软骨修复研究进展 被引量:3
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作者 周斌 樊粤光 《中国中医骨伤科杂志》 CAS 2007年第10期67-69,共3页
关键词 骨髓干细胞 软骨修复 研究 进展
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