The effect of acupuncture and moxibustion on myelogenic osteoclastogenesis and IL-6 mRNA expression is investigated. The result turns to be that the number of myelogenic osteoclasts in the model group is obviously big...The effect of acupuncture and moxibustion on myelogenic osteoclastogenesis and IL-6 mRNA expression is investigated. The result turns to be that the number of myelogenic osteoclasts in the model group is obviously bigger than in the sham operation group (P<0.01), and that in the acupuncture group is markedly smaller than in the model group (P<0.01). The IL-6 mRNA expression in the marrow cells of the model group is significantly elevated, as compared with that in the sham operation group (P<0.01), while the its elevation is lower in the acupuncture group than in the model group (P<0.01). It is therefore concluded that 1) acupuncture effectively modulates the secretion of and then to reduces IL-6 mRNA expression and 2) acupuncture decreases the number of myelogenic osteoclasts.展开更多
This study examined the signaling events induced by shikonin that lead to the induction of apoptosis in Bcr/ Abl-positive chronic myelogenous leukemia （CML） cells （e.g., K562, LAMA84）. Treatment of K562 cells with...This study examined the signaling events induced by shikonin that lead to the induction of apoptosis in Bcr/ Abl-positive chronic myelogenous leukemia （CML） cells （e.g., K562, LAMA84）. Treatment of K562 cells with shikonin （e.g., 0.5 pM） resulted in profound induction of apoptosis accompanied by rapid generation of reactive oxygen species （ROS）, striking activation of c-Jun-N-terminal kinase （JNK） and p38, marked release of the mitochondrial proteins cytochrome c and Smac/DIABLO, activation of caspase-9 and -3, and cleavage of PARP. Scavenging of ROS completely blocked all of the above-mentioned events （i.e., JNK and p38 phosphorylation, cytochrome c and Smac/DIABLO release, caspase and PARP cleavage, as well as the induction of apoptosis） following shikonin treatment. Inhibition of JNK and knock-down of JNK1 significantly attenuated cytochrome c release, caspase cleavage and apoptosis, but did not affect shikonin-mediated ROS production. Additionally, inhibition of caspase activation completely blocked shikonin-induced apoptosis, but did not appreciably modify shikonin-mediated cytochrome c release or ROS generation. Altogether, these findings demonstrate that shikonin-induced oxidative injury operates at a proximal point in apoptotic signaling cascades, and subsequently activates the stress-related JNK pathway, triggers mitochondrial dysfunction, cytochrome c release, and caspase activation, and leads to apoptosis. Our data also suggest that shikonin may be a promising agent for the treatment of CML, as a generator of ROS.展开更多
Background. Granulocytic sarcoma of the cervix, leading to the diagnosis of acute myelogenous leukemia (AML), is a rare event. Case. A 33- year- old woman presented with vaginal bleeding, pain, and a large pelvic mass...Background. Granulocytic sarcoma of the cervix, leading to the diagnosis of acute myelogenous leukemia (AML), is a rare event. Case. A 33- year- old woman presented with vaginal bleeding, pain, and a large pelvic mass involving the cervix, extending bilaterally to the pelvic side walls. A biopsy of the cervix revealed granulocytic sarcoma, and a subsequent bone marrow biopsy confirmed the diagnosis of AML. Results. The majority of patients with cervical granulocytic sarcoma present with vaginal bleeding, sometimes with abdominal pain and other systemic symptoms. The overall 2- year survival rates for all patients with granulocytic sarcoma in the literature is 6% , and none of the patients lived 5 years. Conclusion. Granulocytic sarcoma of the cervix is rare. Awareness of this entity will allow earlier diagnosis and appropriate treatment.展开更多
文摘The effect of acupuncture and moxibustion on myelogenic osteoclastogenesis and IL-6 mRNA expression is investigated. The result turns to be that the number of myelogenic osteoclasts in the model group is obviously bigger than in the sham operation group (P<0.01), and that in the acupuncture group is markedly smaller than in the model group (P<0.01). The IL-6 mRNA expression in the marrow cells of the model group is significantly elevated, as compared with that in the sham operation group (P<0.01), while the its elevation is lower in the acupuncture group than in the model group (P<0.01). It is therefore concluded that 1) acupuncture effectively modulates the secretion of and then to reduces IL-6 mRNA expression and 2) acupuncture decreases the number of myelogenic osteoclasts.
基金Acknowledgments This work was supported by grants from the National High Technology Research and Development Program of China （863 Program） （2006AA02A306）, the National Natural Science Foundation of China （No. 39900082） and the PhD Program Foundation of Ministry of Education of China （No. 204090188）. We thank Dr Courtney M Heney （Massachusetts General Hospital, Harvard University） for critically reading the manuscript.
文摘This study examined the signaling events induced by shikonin that lead to the induction of apoptosis in Bcr/ Abl-positive chronic myelogenous leukemia （CML） cells （e.g., K562, LAMA84）. Treatment of K562 cells with shikonin （e.g., 0.5 pM） resulted in profound induction of apoptosis accompanied by rapid generation of reactive oxygen species （ROS）, striking activation of c-Jun-N-terminal kinase （JNK） and p38, marked release of the mitochondrial proteins cytochrome c and Smac/DIABLO, activation of caspase-9 and -3, and cleavage of PARP. Scavenging of ROS completely blocked all of the above-mentioned events （i.e., JNK and p38 phosphorylation, cytochrome c and Smac/DIABLO release, caspase and PARP cleavage, as well as the induction of apoptosis） following shikonin treatment. Inhibition of JNK and knock-down of JNK1 significantly attenuated cytochrome c release, caspase cleavage and apoptosis, but did not affect shikonin-mediated ROS production. Additionally, inhibition of caspase activation completely blocked shikonin-induced apoptosis, but did not appreciably modify shikonin-mediated cytochrome c release or ROS generation. Altogether, these findings demonstrate that shikonin-induced oxidative injury operates at a proximal point in apoptotic signaling cascades, and subsequently activates the stress-related JNK pathway, triggers mitochondrial dysfunction, cytochrome c release, and caspase activation, and leads to apoptosis. Our data also suggest that shikonin may be a promising agent for the treatment of CML, as a generator of ROS.
文摘Background. Granulocytic sarcoma of the cervix, leading to the diagnosis of acute myelogenous leukemia (AML), is a rare event. Case. A 33- year- old woman presented with vaginal bleeding, pain, and a large pelvic mass involving the cervix, extending bilaterally to the pelvic side walls. A biopsy of the cervix revealed granulocytic sarcoma, and a subsequent bone marrow biopsy confirmed the diagnosis of AML. Results. The majority of patients with cervical granulocytic sarcoma present with vaginal bleeding, sometimes with abdominal pain and other systemic symptoms. The overall 2- year survival rates for all patients with granulocytic sarcoma in the literature is 6% , and none of the patients lived 5 years. Conclusion. Granulocytic sarcoma of the cervix is rare. Awareness of this entity will allow earlier diagnosis and appropriate treatment.
