Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG A...Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG AT-3′),pCI2(+)(5′-GCX ACX AAX ATX ATX GAX AA-3′),pCI1(+)(5′-GTX GGX TCX GGX AAX TCX AC-3′)and pHC(+)(5′-TGY GAY AAY CAZ TTX GA-3′)(X=A,T,C or G:Y=T or C;Z=A or G;N=A or T;M=A,T or G)Using degenerated PCR and modified RACE methods,a protocol for determination of complete genome sequence of potyviruses was established and proved to be successful on five展开更多
Sugarcane mosaic disease, caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Maize dwarf mosaic virus (MDMV) or Johnsongrass mosaic virus (JGMV) in Potyvirus, is one of the most important viral dise...Sugarcane mosaic disease, caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Maize dwarf mosaic virus (MDMV) or Johnsongrass mosaic virus (JGMV) in Potyvirus, is one of the most important viral diseases of sugarcane. In the study, four primer pairs specific to SCMV, SrMV, MDMV and JGMV, respectively, were designed and used to detect 29 sugarcane leaf mosaic samples collected from 9 locations in Jiangxi province. The representative RT-PCR products were sequenced. The results showed that 22 samples were infected by SCMV, three by SrMV, and four were mix-infected by SCMV and SrMV. MDMV or JGMV were not identified in all samples. The result indicates that SCMV is the major pathogen of sugarcane mosaic disease in Jiangxi province, and SrMV is also a pathogen for the disease.展开更多
A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) method was developed for the simultaneous detection and discrimination of three sweet potato potyviruses: Sweet potato feathery mottle vir...A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) method was developed for the simultaneous detection and discrimination of three sweet potato potyviruses: Sweet potato feathery mottle virus(SPFMV),Sweet potato latent virus (SPLV) and Sweet potato virus G (SPVG). Three compatible sets of primers specific for each virus were designed in conserved regions of the coat protein (CP) gene for use in multiplex RT-PCR assay, and producing three distinct fragments 300, 420, and 600 bp, indicating the presence of SPFMV, SPLV and SPVG respectively. The individual RT-PCR assays and the multiplex assay were optimized for highest sensitivity and specificity. This study fulfilled the need for rapid and specific sweet potato potyvirus diagnostic tool and that also had the potential for investigating the epidemiology of sweet viral diseases.展开更多
【目的】马铃薯Y病毒属(Potyvirus)是世界上最大的两个植物病毒属之一,对多种农作物造成危害。利用文献数据库,客观地分析国内外Potyvirus研究的发展动态,为国内外Potyvirus科研工作者和决策者提供参考。【方法】基于Web of Science数据...【目的】马铃薯Y病毒属(Potyvirus)是世界上最大的两个植物病毒属之一,对多种农作物造成危害。利用文献数据库,客观地分析国内外Potyvirus研究的发展动态,为国内外Potyvirus科研工作者和决策者提供参考。【方法】基于Web of Science数据库,采用文献计量学方法,对全球发表于1985 2012年的Potyvirus文献的国家、作者、机构、载文期刊及研究内容进行了数量和质量分析。【结果】检索到全球60个国家发表的Potyvirus文献2 442篇,以及每篇文献的被引频次和每个国家、机构及作者的H指数。经分析得出:全球Potyvirus研究论文的产出量自1991年呈稳定上升趋势,文献的数量和质量在统计的国家中,以美国最好,其次是法国、西班牙、澳大利亚、英国;美国的高产活跃作者最多;Potyvirus研究的热点是基因沉默和分子生物学,并随着研究技术方法的革新将不断深入;研究方向更多倾向于寄主方面的研究(如基因沉默、寄主抗性、与寄主互作等)及交叉学科;而中国在Potyvirus基础研究上距离国际先进水平还有很大距离,研究力量较少,有影响力的论文较少。【结论】美国在该领域的研究处于领先水平,我国仍需加强Potyvirus基础研究力量投入,努力缩短差距。展开更多
The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was e...The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was expected to encode part C-terminus of NIb protein of 201 amino acids,complete capsid protein(CP)of 276 amino acids and followed by a 3’ untranslated region(UTR)of 168 nucleotides.The comparison of sequence diversity among BtMV-XJ and other three previously reported isolates revealed that it shared 98.3%,98.1%,91.4% nucleotide identity,and 99.79%,99.58%,97.69% deduced aa sequence identity with the corresponding 3’ terminal regions of BtMV-SL,BtMV-UK and BtMV-US,respectively,and that most nucleotide mutations were silent with no effect on aa sequence.In addition,a virus-specific and ra-pid RT-PCR detection method of BtMV was also developed based on the nucleotide sequence obtained.展开更多
文摘Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG AT-3′),pCI2(+)(5′-GCX ACX AAX ATX ATX GAX AA-3′),pCI1(+)(5′-GTX GGX TCX GGX AAX TCX AC-3′)and pHC(+)(5′-TGY GAY AAY CAZ TTX GA-3′)(X=A,T,C or G:Y=T or C;Z=A or G;N=A or T;M=A,T or G)Using degenerated PCR and modified RACE methods,a protocol for determination of complete genome sequence of potyviruses was established and proved to be successful on five
文摘Sugarcane mosaic disease, caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Maize dwarf mosaic virus (MDMV) or Johnsongrass mosaic virus (JGMV) in Potyvirus, is one of the most important viral diseases of sugarcane. In the study, four primer pairs specific to SCMV, SrMV, MDMV and JGMV, respectively, were designed and used to detect 29 sugarcane leaf mosaic samples collected from 9 locations in Jiangxi province. The representative RT-PCR products were sequenced. The results showed that 22 samples were infected by SCMV, three by SrMV, and four were mix-infected by SCMV and SrMV. MDMV or JGMV were not identified in all samples. The result indicates that SCMV is the major pathogen of sugarcane mosaic disease in Jiangxi province, and SrMV is also a pathogen for the disease.
文摘A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) method was developed for the simultaneous detection and discrimination of three sweet potato potyviruses: Sweet potato feathery mottle virus(SPFMV),Sweet potato latent virus (SPLV) and Sweet potato virus G (SPVG). Three compatible sets of primers specific for each virus were designed in conserved regions of the coat protein (CP) gene for use in multiplex RT-PCR assay, and producing three distinct fragments 300, 420, and 600 bp, indicating the presence of SPFMV, SPLV and SPVG respectively. The individual RT-PCR assays and the multiplex assay were optimized for highest sensitivity and specificity. This study fulfilled the need for rapid and specific sweet potato potyvirus diagnostic tool and that also had the potential for investigating the epidemiology of sweet viral diseases.
文摘【目的】马铃薯Y病毒属(Potyvirus)是世界上最大的两个植物病毒属之一,对多种农作物造成危害。利用文献数据库,客观地分析国内外Potyvirus研究的发展动态,为国内外Potyvirus科研工作者和决策者提供参考。【方法】基于Web of Science数据库,采用文献计量学方法,对全球发表于1985 2012年的Potyvirus文献的国家、作者、机构、载文期刊及研究内容进行了数量和质量分析。【结果】检索到全球60个国家发表的Potyvirus文献2 442篇,以及每篇文献的被引频次和每个国家、机构及作者的H指数。经分析得出:全球Potyvirus研究论文的产出量自1991年呈稳定上升趋势,文献的数量和质量在统计的国家中,以美国最好,其次是法国、西班牙、澳大利亚、英国;美国的高产活跃作者最多;Potyvirus研究的热点是基因沉默和分子生物学,并随着研究技术方法的革新将不断深入;研究方向更多倾向于寄主方面的研究(如基因沉默、寄主抗性、与寄主互作等)及交叉学科;而中国在Potyvirus基础研究上距离国际先进水平还有很大距离,研究力量较少,有影响力的论文较少。【结论】美国在该领域的研究处于领先水平,我国仍需加强Potyvirus基础研究力量投入,努力缩短差距。
文摘The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was expected to encode part C-terminus of NIb protein of 201 amino acids,complete capsid protein(CP)of 276 amino acids and followed by a 3’ untranslated region(UTR)of 168 nucleotides.The comparison of sequence diversity among BtMV-XJ and other three previously reported isolates revealed that it shared 98.3%,98.1%,91.4% nucleotide identity,and 99.79%,99.58%,97.69% deduced aa sequence identity with the corresponding 3’ terminal regions of BtMV-SL,BtMV-UK and BtMV-US,respectively,and that most nucleotide mutations were silent with no effect on aa sequence.In addition,a virus-specific and ra-pid RT-PCR detection method of BtMV was also developed based on the nucleotide sequence obtained.
