Seedlings of the gymnosperm, Pinus edulis Engelm., have a distinctive pattern of starch accumulation following germination; however, the enzymes involved in starch synthesis have not been studied in gymnosperm sp...Seedlings of the gymnosperm, Pinus edulis Engelm., have a distinctive pattern of starch accumulation following germination; however, the enzymes involved in starch synthesis have not been studied in gymnosperm species. In this study, enzymes and starch were extracted from P. edulis seedlings germinated in the dark at room temperature. Granule_bound proteins of 58 kD and 91 kD were recognized by a pea SS Ⅱ antiserum. The 58 kD granule_bound protein was purified and identified as granule_bound starch synthase Ⅰ by alignment of the N_terminal sequence with that of granule_bound starch synthase Ⅰ from several angiosperms. Elution of soluble starch synthase activity from a DEAE_Sepharose column showed two starch synthase activity peaks, indicating at least two isoforms of soluble starch synthases. Primer affinities of soluble starch synthases were investigated. Glycogen from rabbit was the best primer for soluble starch synthase. The enzymological properties of Pinus starch synthases appear to be similar to those reported for angiosperms.展开更多
美国可食松P inus edu lis是自美国近两年引进的一种常绿针叶灌木树种.对其1年生播种苗进行了全年的生长观测、生物量测定以及形态特点的研究,并以本地树种油松P inus tabu laef orm is 1年生播种苗作为对比,将可食松1年生苗的生长划分...美国可食松P inus edu lis是自美国近两年引进的一种常绿针叶灌木树种.对其1年生播种苗进行了全年的生长观测、生物量测定以及形态特点的研究,并以本地树种油松P inus tabu laef orm is 1年生播种苗作为对比,将可食松1年生苗的生长划分为4个时期(出苗期,幼嫩期,速生期和苗木硬化期).结果表明:可食松1年生苗高生长速生期全年仅1次,地径速生期则出现2次;可食松苗地上部分粗壮矮小、初生叶繁多,地下部分具深根性特点.同时对该树种抗逆性进行了初步分析.展开更多
文摘Seedlings of the gymnosperm, Pinus edulis Engelm., have a distinctive pattern of starch accumulation following germination; however, the enzymes involved in starch synthesis have not been studied in gymnosperm species. In this study, enzymes and starch were extracted from P. edulis seedlings germinated in the dark at room temperature. Granule_bound proteins of 58 kD and 91 kD were recognized by a pea SS Ⅱ antiserum. The 58 kD granule_bound protein was purified and identified as granule_bound starch synthase Ⅰ by alignment of the N_terminal sequence with that of granule_bound starch synthase Ⅰ from several angiosperms. Elution of soluble starch synthase activity from a DEAE_Sepharose column showed two starch synthase activity peaks, indicating at least two isoforms of soluble starch synthases. Primer affinities of soluble starch synthases were investigated. Glycogen from rabbit was the best primer for soluble starch synthase. The enzymological properties of Pinus starch synthases appear to be similar to those reported for angiosperms.
文摘美国可食松P inus edu lis是自美国近两年引进的一种常绿针叶灌木树种.对其1年生播种苗进行了全年的生长观测、生物量测定以及形态特点的研究,并以本地树种油松P inus tabu laef orm is 1年生播种苗作为对比,将可食松1年生苗的生长划分为4个时期(出苗期,幼嫩期,速生期和苗木硬化期).结果表明:可食松1年生苗高生长速生期全年仅1次,地径速生期则出现2次;可食松苗地上部分粗壮矮小、初生叶繁多,地下部分具深根性特点.同时对该树种抗逆性进行了初步分析.
