继“人类基因组计划”后生命科学领域最大的国际合作计划之一——“DNA元件百科全书”计划(Encyclopedia of DNAElements,ENCODE)日前发表了一系列重要文章报告了其示范期完成的1%人类基因组的解码情况.这些成果由来自11个国家的35个小...继“人类基因组计划”后生命科学领域最大的国际合作计划之一——“DNA元件百科全书”计划(Encyclopedia of DNAElements,ENCODE)日前发表了一系列重要文章报告了其示范期完成的1%人类基因组的解码情况.这些成果由来自11个国家的35个小组合作完成,挑战了人们对于人类基因组的传统认识,即人类基因组不是由孤立的基因和大量“无用DNA片段”组成的,基因组本身就是一个复杂的网络系统,有着多层次的精细调控规则.ENCODE计划示范期(1%人类基因组解码)的成果为进一步解译人类基因组这部“天书”开辟了道路,刷新了我们关于基因的概念,成为人类生物学研究史中又一座里程碑.同时,大量的“非编码RNA基因”仍旧是生命科学天空中的“一朵乌云”,其未知的功能尚待探索.展开更多
Whether or not non-coding RNA genes play a significant role in reproductive biology and evolution of sex determination systems is an important problem. We report identification of sex-related non-coding RNA (ncRNA) ge...Whether or not non-coding RNA genes play a significant role in reproductive biology and evolution of sex determination systems is an important problem. We report identification of sex-related non-coding RNA (ncRNA) genes and an analysis of ncRNAs expression patterns among Drosophila species. We detected 12 candidate ncRNAs that are expressed in the gonads of D. melanogaster by an integrative approach of RT-PCR and computational analysis of sequence conservation across species. We ex-perimentally analyzed these ncRNA gene transcripts in head, ovary and testis of closely related species D. simulans, D. yakuba,D. pseudoobscura and D. virilis. We observed that the occurrence and extent of expression of most ncRNA fragments among closely related species show significant divergence.展开更多
文摘继“人类基因组计划”后生命科学领域最大的国际合作计划之一——“DNA元件百科全书”计划(Encyclopedia of DNAElements,ENCODE)日前发表了一系列重要文章报告了其示范期完成的1%人类基因组的解码情况.这些成果由来自11个国家的35个小组合作完成,挑战了人们对于人类基因组的传统认识,即人类基因组不是由孤立的基因和大量“无用DNA片段”组成的,基因组本身就是一个复杂的网络系统,有着多层次的精细调控规则.ENCODE计划示范期(1%人类基因组解码)的成果为进一步解译人类基因组这部“天书”开辟了道路,刷新了我们关于基因的概念,成为人类生物学研究史中又一座里程碑.同时,大量的“非编码RNA基因”仍旧是生命科学天空中的“一朵乌云”,其未知的功能尚待探索.
基金Supported by the National Natural Science Foundation of China (Grant Nos. 30550004 and 30510403188)the Changjiang Scholars Foundation of Peking Uni-versity and China Ministry of Education Program of Introducing Talents of Disci-pline to Universities (Grant No. B06001)
文摘Whether or not non-coding RNA genes play a significant role in reproductive biology and evolution of sex determination systems is an important problem. We report identification of sex-related non-coding RNA (ncRNA) genes and an analysis of ncRNAs expression patterns among Drosophila species. We detected 12 candidate ncRNAs that are expressed in the gonads of D. melanogaster by an integrative approach of RT-PCR and computational analysis of sequence conservation across species. We ex-perimentally analyzed these ncRNA gene transcripts in head, ovary and testis of closely related species D. simulans, D. yakuba,D. pseudoobscura and D. virilis. We observed that the occurrence and extent of expression of most ncRNA fragments among closely related species show significant divergence.
