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RAPD analysis of alfalfa DNA mutation via N^+ implantation 被引量:3
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作者 LIYu-Feng HUANGQun-Ce +1 位作者 LIANGYun-Zhang YUZeng-Liang 《Nuclear Science and Techniques》 SCIE CAS CSCD 2003年第3期161-163,共3页
Germination capacity of alfalfa seeds under low energy N+ implantation manifests oscillations goingdown with dose strength. From analyzing alfalfa genome DNA under low energy N+ implantation by RAPD (RandomAmplified P... Germination capacity of alfalfa seeds under low energy N+ implantation manifests oscillations goingdown with dose strength. From analyzing alfalfa genome DNA under low energy N+ implantation by RAPD (RandomAmplified Polymorphous DNA), it is recommended that 30 polymorphic DNA fragments be amplified with 8 primersin total 100 primers, and fluorescence intensity of the identical DNA fragments amplified by RAPD is different be-tween CK and treatments. Number of different polymorphic DNA fragments between treatment and CK via N+ im-plantation manifests going up with dose strength. 展开更多
关键词 RAPD 紫花苜蓿 基因突变 氮离子注入 随机放大多晶dna
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