In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation,shoots of geranium were exposed at dosages of 0,10,15,30,and40...In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation,shoots of geranium were exposed at dosages of 0,10,15,30,and40 Gy,and one flower color mutant was obtained.The morphological characteristics,physiological aspects,and DNA polymorphisms between wild-type and flower color mutants were analyzed.The colors of petal,peduncle,pistil,and stamen of the mutant displayed significant differences compared to those of the wild-type.Compared to the original plants,the total anthocyanin content in the petals of the mutant significantly decreased,resulting in a light pink petal phenotype.DNA polymorphisms detected by random amplified polymorphic DNA analysis showed that the ratio of different bands between the wild-type and mutant reached up to 13.2%.The present study demonstrates that carbon ion beam irradiation is effective in inducing genomic variations,resulting in flower color geranium mutants within a relatively short period of time.Meanwhile,the developed flower-color mutants may be potentially used in future mutational research studies involving ornamental plants.展开更多
Eel family is a huge one, in which many kinds of eelsespecially some migratory eels, bear strong resemblance toeach other, and are therefOre difficult to be identified. Inthis study 29 random primers were used to make...Eel family is a huge one, in which many kinds of eelsespecially some migratory eels, bear strong resemblance toeach other, and are therefOre difficult to be identified. Inthis study 29 random primers were used to make RAPDana1ysis for Japanese ee1 (Angui11ajaponica). European eel(Anguilla anguilla) and Pike eel (AIllrae1lesox cinereus).And totally 299 fragments were counted. Shared or spe-cific fragments were counted alld ge11etic similarity or ge-netic distance were calculated. The genetic similarity be-tween Japanese eel alld Pike eel is 0.68 and the geneticdistance between them is 0.32; those between Europeaneel and Pike eel are 0.72 and 0.28 respectively, and be-tween Japanese eel and Ellropean eel are 0.74 and 0.25 re-spectively. The method has been shown to be suitable tomolecular identification of eels. It provides an a1ternativeapproach to determine the relatiollship between species.展开更多
Jujube witches＇ broom is a devastating disease of Ziziphusjujube that occurs in various jujube regions of China. Nucleic acid extracted from midribs of samples collected from three jujube varieties （＂Suanzao＂, ＂L...Jujube witches＇ broom is a devastating disease of Ziziphusjujube that occurs in various jujube regions of China. Nucleic acid extracted from midribs of samples collected from three jujube varieties （＂Suanzao＂, ＂Lajiaozao＂ and ＂Langzao＂） from symptomatic and asymptomatic shoots were tested by random amplified polymorphic DNA analyses. Using 13 different 10 and 11-bp random primers the amplification of jujube DNA was achieved from all the samples; AMI4 primer provided amplification of specific DNA fragments of about 400 bp, only from samples collected from symptomatic plants. No genetic variations in these varieties were identified using the other 11 arbitrary primers; only with primer AL07 it was possible to differentiate ＂Langzao＂ from the other two varieties tested. All the experiments were repeated 2 times and the results were consistent. Compared with PCR analyses with phytoplasma-specific primers, RAPD techniques resulted to be an alternative rapid and sensitive method for detecting jujube phytoplasmas presence in different jujube varieties.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.11205218,11275171,and 11405234)the Knowledge Innovation Project of the Chinese Academy of Sciences(CAS)(No.KJCX2-EW-N05)+1 种基金CAS‘‘Light of West China’’Program(No.29Y506020)the Youth Innovation Promotion Association of CAS(No.29Y506030)supported this study
文摘In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation,shoots of geranium were exposed at dosages of 0,10,15,30,and40 Gy,and one flower color mutant was obtained.The morphological characteristics,physiological aspects,and DNA polymorphisms between wild-type and flower color mutants were analyzed.The colors of petal,peduncle,pistil,and stamen of the mutant displayed significant differences compared to those of the wild-type.Compared to the original plants,the total anthocyanin content in the petals of the mutant significantly decreased,resulting in a light pink petal phenotype.DNA polymorphisms detected by random amplified polymorphic DNA analysis showed that the ratio of different bands between the wild-type and mutant reached up to 13.2%.The present study demonstrates that carbon ion beam irradiation is effective in inducing genomic variations,resulting in flower color geranium mutants within a relatively short period of time.Meanwhile,the developed flower-color mutants may be potentially used in future mutational research studies involving ornamental plants.
文摘Eel family is a huge one, in which many kinds of eelsespecially some migratory eels, bear strong resemblance toeach other, and are therefOre difficult to be identified. Inthis study 29 random primers were used to make RAPDana1ysis for Japanese ee1 (Angui11ajaponica). European eel(Anguilla anguilla) and Pike eel (AIllrae1lesox cinereus).And totally 299 fragments were counted. Shared or spe-cific fragments were counted alld ge11etic similarity or ge-netic distance were calculated. The genetic similarity be-tween Japanese eel alld Pike eel is 0.68 and the geneticdistance between them is 0.32; those between Europeaneel and Pike eel are 0.72 and 0.28 respectively, and be-tween Japanese eel and Ellropean eel are 0.74 and 0.25 re-spectively. The method has been shown to be suitable tomolecular identification of eels. It provides an a1ternativeapproach to determine the relatiollship between species.
文摘Jujube witches＇ broom is a devastating disease of Ziziphusjujube that occurs in various jujube regions of China. Nucleic acid extracted from midribs of samples collected from three jujube varieties （＂Suanzao＂, ＂Lajiaozao＂ and ＂Langzao＂） from symptomatic and asymptomatic shoots were tested by random amplified polymorphic DNA analyses. Using 13 different 10 and 11-bp random primers the amplification of jujube DNA was achieved from all the samples; AMI4 primer provided amplification of specific DNA fragments of about 400 bp, only from samples collected from symptomatic plants. No genetic variations in these varieties were identified using the other 11 arbitrary primers; only with primer AL07 it was possible to differentiate ＂Langzao＂ from the other two varieties tested. All the experiments were repeated 2 times and the results were consistent. Compared with PCR analyses with phytoplasma-specific primers, RAPD techniques resulted to be an alternative rapid and sensitive method for detecting jujube phytoplasmas presence in different jujube varieties.
