11.1 Pancreatitis2007240 Role of acinar cell apoptosis and expression of Bax and Caspase-8 in rats with acute pancreatitis. CHEN Hailong(陈海龙), et al. Dept Surg, 1st Affili Hosp, Dalian Med Univ, Dalian 116011. Wo...11.1 Pancreatitis2007240 Role of acinar cell apoptosis and expression of Bax and Caspase-8 in rats with acute pancreatitis. CHEN Hailong(陈海龙), et al. Dept Surg, 1st Affili Hosp, Dalian Med Univ, Dalian 116011. World Chin J Dig 2007;15(10):1067-1072. Objective To investigate the apoptosis of pancreatic acinar cells and the expression of apoptosis-regulated genes Bax and Caspase-8 in experimental acute pancreatitis and their correlations with the severity of the disease in rats. Methods The models of acute edematous pancreatitis (AEP) and acute necrotizing pancreatitis (ANP) were established by retrograde injection of different concentrations of sodium deoxycholate into the common biliary and pancreatic duct, and then the rats were randomly divided into 3 groups (10 for each): sham operated group (control), AEP group (treated with 7.5 g/L sodium deoxycholate) and ANP group (treated with 15 g/L sodium deoxycholat). The apoptosis of acinar cells was determined by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay. The amounts of Bax and Caspase-8 mRNA expression in the pancreas were analyzed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). The expression of Bax and Caspase-8 protein were detected by immunohistochemical method. The levels of serum amylase, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were also measured respectively. Results The levels of serum amylase (63 413±6118, 1 532 134±17 654 nkat/L vs 15 736±483 nkat/L, P【0.01), TNF-α (1.86±0.13, 2.97±0.14 μg/L vs 0.95±0.08 μg/L, P【0.01) and IL-6 (47.10 ±7.05, 170.10±7.59 ng/L vs 29.20±4.47 ng/L, P【0.01) in AEP and ANP group were significantly higher than those in control group, and there were also significant differences between ANP and AEP group (P【0.05). The apoptotic index (AI) in both AEP and ANP group was obviously higher than that in control group (22.09±3.71, 6.50±1.58 vs 0.13±0.05, P【0.01), and there was also marked difference between ANP an展开更多
文摘11.1 Pancreatitis2007240 Role of acinar cell apoptosis and expression of Bax and Caspase-8 in rats with acute pancreatitis. CHEN Hailong(陈海龙), et al. Dept Surg, 1st Affili Hosp, Dalian Med Univ, Dalian 116011. World Chin J Dig 2007;15(10):1067-1072. Objective To investigate the apoptosis of pancreatic acinar cells and the expression of apoptosis-regulated genes Bax and Caspase-8 in experimental acute pancreatitis and their correlations with the severity of the disease in rats. Methods The models of acute edematous pancreatitis (AEP) and acute necrotizing pancreatitis (ANP) were established by retrograde injection of different concentrations of sodium deoxycholate into the common biliary and pancreatic duct, and then the rats were randomly divided into 3 groups (10 for each): sham operated group (control), AEP group (treated with 7.5 g/L sodium deoxycholate) and ANP group (treated with 15 g/L sodium deoxycholat). The apoptosis of acinar cells was determined by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay. The amounts of Bax and Caspase-8 mRNA expression in the pancreas were analyzed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). The expression of Bax and Caspase-8 protein were detected by immunohistochemical method. The levels of serum amylase, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were also measured respectively. Results The levels of serum amylase (63 413±6118, 1 532 134±17 654 nkat/L vs 15 736±483 nkat/L, P【0.01), TNF-α (1.86±0.13, 2.97±0.14 μg/L vs 0.95±0.08 μg/L, P【0.01) and IL-6 (47.10 ±7.05, 170.10±7.59 ng/L vs 29.20±4.47 ng/L, P【0.01) in AEP and ANP group were significantly higher than those in control group, and there were also significant differences between ANP and AEP group (P【0.05). The apoptotic index (AI) in both AEP and ANP group was obviously higher than that in control group (22.09±3.71, 6.50±1.58 vs 0.13±0.05, P【0.01), and there was also marked difference between ANP an
