植物钙依赖的蛋白激酶(Calcium-dependent protein kinases,CDPKs)是细胞Ca2+信号的受体,同时具有Ca2+受体蛋白和Ser/Thr蛋白激酶的功能。许多植物CDPKs基因受环境胁迫刺激发生表达水平的改变,这些基因在植物逆境胁迫的Ca2+信号转导中...植物钙依赖的蛋白激酶(Calcium-dependent protein kinases,CDPKs)是细胞Ca2+信号的受体,同时具有Ca2+受体蛋白和Ser/Thr蛋白激酶的功能。许多植物CDPKs基因受环境胁迫刺激发生表达水平的改变,这些基因在植物逆境胁迫的Ca2+信号转导中起着十分重要的作用,为植物CDPKs抗逆功能的研究和植物的抗逆遗传改良提供了理论基础和基因资源。展开更多
As calcium sensors, plant calcium-dependent protein kinases(CDPKs) play important roles in plants' responses to various abiotic stresses. Here, we report the functional characterization of CPK28, a member of the C...As calcium sensors, plant calcium-dependent protein kinases(CDPKs) play important roles in plants' responses to various abiotic stresses. Here, we report the functional characterization of CPK28, a member of the CDPK family in Arabidopsis, in response to osmotic stress.The cpk28 mutant, a loss-of-function mutant, exhibited an NaCl- and mannitol-sensitive phenotype in green cotyledons, while CPK28-overexpressing plants displayed stronger tolerance to NaCl and mannitol stresses than wildtype plants. Reverse transcriptase-polymerase chain reaction and beta-glucuronidase staining assays showed that NaCl and mannitol stresses induced CPK28. CPK28-overexpressing lines accumulated significantly more proline relative to wild-type plants and mutant plants under NaCl and mannitol stresses. Transient expression of CPK28-GFP in mesophyll cell protoplasts, as well as stable transgenic lines expressing CPK28-GFP, showed that CPK28 was localized in the plasma membrane. Expression levels of known stress-responsive genes were not significantly altered in the null mutant and overexpression lines,suggesting that CPK28 possibly mediated the stress response via the regulation of target proteins rather than via regulation at the level of transcription. Meanwhile, CPK28could autophosphorylate. Taken together, these data demonstrated that CPK28, a potential positive regulator, is involved in the response to osmotic stress in Arabidopsis.展开更多
文摘植物钙依赖的蛋白激酶(Calcium-dependent protein kinases,CDPKs)是细胞Ca2+信号的受体,同时具有Ca2+受体蛋白和Ser/Thr蛋白激酶的功能。许多植物CDPKs基因受环境胁迫刺激发生表达水平的改变,这些基因在植物逆境胁迫的Ca2+信号转导中起着十分重要的作用,为植物CDPKs抗逆功能的研究和植物的抗逆遗传改良提供了理论基础和基因资源。
基金supported by the National Genetically Modified Organisms Breeding Major Projects(2011ZX08009-002)
文摘As calcium sensors, plant calcium-dependent protein kinases(CDPKs) play important roles in plants' responses to various abiotic stresses. Here, we report the functional characterization of CPK28, a member of the CDPK family in Arabidopsis, in response to osmotic stress.The cpk28 mutant, a loss-of-function mutant, exhibited an NaCl- and mannitol-sensitive phenotype in green cotyledons, while CPK28-overexpressing plants displayed stronger tolerance to NaCl and mannitol stresses than wildtype plants. Reverse transcriptase-polymerase chain reaction and beta-glucuronidase staining assays showed that NaCl and mannitol stresses induced CPK28. CPK28-overexpressing lines accumulated significantly more proline relative to wild-type plants and mutant plants under NaCl and mannitol stresses. Transient expression of CPK28-GFP in mesophyll cell protoplasts, as well as stable transgenic lines expressing CPK28-GFP, showed that CPK28 was localized in the plasma membrane. Expression levels of known stress-responsive genes were not significantly altered in the null mutant and overexpression lines,suggesting that CPK28 possibly mediated the stress response via the regulation of target proteins rather than via regulation at the level of transcription. Meanwhile, CPK28could autophosphorylate. Taken together, these data demonstrated that CPK28, a potential positive regulator, is involved in the response to osmotic stress in Arabidopsis.
