A new approach for separation of intact proteins with high resolution, high speed and high sample loading ("three highs") by non-porous packings in high performance liquid chromatography is presented.A chrom...A new approach for separation of intact proteins with high resolution, high speed and high sample loading ("three highs") by non-porous packings in high performance liquid chromatography is presented.A chromatographic cake having larger diameter than its thickness is firstly employed to completely separate 1.0 and 40 μg of the mixture of 7 standard proteins within 1 min un-der conventional chromatographic conditions.Also, 0.5 mg of the mixture was almost completely separated within 1 min, accom-plishing the "three high" purpose.This research expands the application utilized for separation of intact proteins with non-porous packings.A smaller geometric size of chromatographic cake packed with much smaller particles of non-porous packings may improve results.展开更多
0003816用 FPGA 实现多路信号跟踪[刊]/朱丽娟//舰船电子对抗.—1999,(6).—5～8(D)0003817200Msps 通用信号采集 PC 卡的研制[刊]/王永钢//数据采集与处理.—1999,14(4),—513～515(D)介绍一种通用高速信号采集 PC 机插卡,它应用了高...0003816用 FPGA 实现多路信号跟踪[刊]/朱丽娟//舰船电子对抗.—1999,(6).—5～8(D)0003817200Msps 通用信号采集 PC 卡的研制[刊]/王永钢//数据采集与处理.—1999,14(4),—513～515(D)介绍一种通用高速信号采集 PC 机插卡,它应用了高速模数变换、可编程逻辑设计,锁相环时钟等新技术。采样分辨率8bit,最高采样率200Msps,卡上存储器长度512KB,具有灵活的触发记录和数据传输方式。配套工作软件在 Windows95平台上实现。该采集卡及软件支持多通道同时使用。展开更多
基金supported by the Modern Separation Science in Shaanxi Provincial Key Laboratory Foundation (09JS092)the Shaanxi Provin-cial Construction of Key Disciplines of Analytical Chemistry Foundation (05JS60)
文摘A new approach for separation of intact proteins with high resolution, high speed and high sample loading ("three highs") by non-porous packings in high performance liquid chromatography is presented.A chromatographic cake having larger diameter than its thickness is firstly employed to completely separate 1.0 and 40 μg of the mixture of 7 standard proteins within 1 min un-der conventional chromatographic conditions.Also, 0.5 mg of the mixture was almost completely separated within 1 min, accom-plishing the "three high" purpose.This research expands the application utilized for separation of intact proteins with non-porous packings.A smaller geometric size of chromatographic cake packed with much smaller particles of non-porous packings may improve results.
文摘0003816用 FPGA 实现多路信号跟踪[刊]/朱丽娟//舰船电子对抗.—1999,(6).—5～8(D)0003817200Msps 通用信号采集 PC 卡的研制[刊]/王永钢//数据采集与处理.—1999,14(4),—513～515(D)介绍一种通用高速信号采集 PC 机插卡,它应用了高速模数变换、可编程逻辑设计,锁相环时钟等新技术。采样分辨率8bit,最高采样率200Msps,卡上存储器长度512KB,具有灵活的触发记录和数据传输方式。配套工作软件在 Windows95平台上实现。该采集卡及软件支持多通道同时使用。
