To gain wholesale information about antineoplastic mechanism of parvovirus, a complementary DNA microarray, representing 12800 different genes, was used to compare the gene expression variance between a H1 sensitive h...To gain wholesale information about antineoplastic mechanism of parvovirus, a complementary DNA microarray, representing 12800 different genes, was used to compare the gene expression variance between a H1 sensitive human liver cell line, L02, and a resistant derivative clone, L02R, which survived the infection of 10 m.o.i, parvovirus H1. The prepared cDNAs of L02 and L02R were labeledwith fluorescent dyes, Cy5 and Cy3 respectively, with which the mixed sample gave a red and a green image after hybridization. The two images were merged into one for further analyses.展开更多
文摘To gain wholesale information about antineoplastic mechanism of parvovirus, a complementary DNA microarray, representing 12800 different genes, was used to compare the gene expression variance between a H1 sensitive human liver cell line, L02, and a resistant derivative clone, L02R, which survived the infection of 10 m.o.i, parvovirus H1. The prepared cDNAs of L02 and L02R were labeledwith fluorescent dyes, Cy5 and Cy3 respectively, with which the mixed sample gave a red and a green image after hybridization. The two images were merged into one for further analyses.
