The impacts of different factors (including light, temperature, nitrogen, phosphorous, iron, carbon, zinc, boron, cobalt, copper, molybdenum, manganess, etc) on cell growth of Nannochloropsis salina, the sole marine g...The impacts of different factors (including light, temperature, nitrogen, phosphorous, iron, carbon, zinc, boron, cobalt, copper, molybdenum, manganess, etc) on cell growth of Nannochloropsis salina, the sole marine genus of the Eustigmatophyceae, were carried out in Qingdao and Sigapore with nature seawater. Based on the results obtained, a optimum model good for culturing this unicellular alga was suggested as follows: 120mg/L urea, 6mg/L KH2PO4, 1.5 mg/L Ferric citrate, 0.05 mg/L ZnSO4, 0.01 mg/L CuSO4, 0.1 mg/L NaMoO3, 0.02 mg/L CoCl2, 0.5 mg/L HBO3, 1.0 mg/L MnSO4,25‰～30‰NaCl, continuous illumination indoor or suitable strong light intensity as well as a contant temperature between 25～30℃, or varied temperature between 25～35℃, specific growth rate at 10% ～15%.3.6×1012 to 1.2×1011 cells /L of dense culture of Nannochloropsis salina were achieved by using 10L of tubular coiled photobioreactor,60L, 200L, 500L and 1600 L of flat glass photoreactors, respectively, which was several hundred-fold higher than the cell density of traditional microalgal culture in aquaculture. Specific growth rate, negative correlation with cell density, affected the biomass output in Nannochloropsis salina culture system. 10%～15% of specific growth rate was suggested in application for maximal production.展开更多
文摘The impacts of different factors (including light, temperature, nitrogen, phosphorous, iron, carbon, zinc, boron, cobalt, copper, molybdenum, manganess, etc) on cell growth of Nannochloropsis salina, the sole marine genus of the Eustigmatophyceae, were carried out in Qingdao and Sigapore with nature seawater. Based on the results obtained, a optimum model good for culturing this unicellular alga was suggested as follows: 120mg/L urea, 6mg/L KH2PO4, 1.5 mg/L Ferric citrate, 0.05 mg/L ZnSO4, 0.01 mg/L CuSO4, 0.1 mg/L NaMoO3, 0.02 mg/L CoCl2, 0.5 mg/L HBO3, 1.0 mg/L MnSO4,25‰～30‰NaCl, continuous illumination indoor or suitable strong light intensity as well as a contant temperature between 25～30℃, or varied temperature between 25～35℃, specific growth rate at 10% ～15%.3.6×1012 to 1.2×1011 cells /L of dense culture of Nannochloropsis salina were achieved by using 10L of tubular coiled photobioreactor,60L, 200L, 500L and 1600 L of flat glass photoreactors, respectively, which was several hundred-fold higher than the cell density of traditional microalgal culture in aquaculture. Specific growth rate, negative correlation with cell density, affected the biomass output in Nannochloropsis salina culture system. 10%～15% of specific growth rate was suggested in application for maximal production.
