目的通过膀胱灌注N蛳甲基亚硝基脲(M N U)诱导近交系大鼠膀胱肿瘤。方法选取F344大鼠20只,膀胱灌注M N U,每2周1次,共5次;于第14周处死动物,取膀胱肿瘤组织测M H C蛳I及病理观察。结果M N U组肿瘤发生率为100%;膀胱肿瘤的M H C蛳I表达较...目的通过膀胱灌注N蛳甲基亚硝基脲(M N U)诱导近交系大鼠膀胱肿瘤。方法选取F344大鼠20只,膀胱灌注M N U,每2周1次,共5次;于第14周处死动物,取膀胱肿瘤组织测M H C蛳I及病理观察。结果M N U组肿瘤发生率为100%;膀胱肿瘤的M H C蛳I表达较高(58.60%￣93.80%)。结论M N U可成功诱导F344大鼠膀胱肿瘤的形成,是肿瘤免疫治疗的理想模型。展开更多
Monitoring method of biochemical markers is inferred corresponding gene changed according to protein changed in laboratory animals, and have definite limitation in some extent. But DNA fingerprints and microsatellite ...Monitoring method of biochemical markers is inferred corresponding gene changed according to protein changed in laboratory animals, and have definite limitation in some extent. But DNA fingerprints and microsatellite DNA directly monitored DNA of genetic materials, more accurate、more reliable and direct than biochemical markers. In the experiments six domestic strains(SHR、SHR/sp、WKY、LEW、RCS、F344) of inbred rat were analyzed DNA polymorphism using DNA fingerprints and microsatellite DNA, and set up the exact、reliable、speedy、convenient method of in the heredity monitoring of inbred rats. The result indicated:(1)DNA fingerprints: ①There were 14-19 distinguishable bands on 4.0kb-23.1kb every DNA fingerprinting. The similarity coefficient(F) and the probability of band sharing(X) were more than 0.9 in different individual of the identical inbred rat strain. and indicated more high similarity in genetic materials of the various individual in same group rats. ②The similarity coefficient and the probability of band sharing of DNA fingerprinting were less than 0.07, probability of the identical DNA fingerprinting was less than 10 -22 among the different individuals of different inbred strain. and indicated that there was more different in genetic materials of various individual in different inbred rat groups. ③)DNA fingerprinting obtained by four times experiment from same individual genome are almost coincident DNA fingerprinting from various tissues are also coincident in the same individual of 6 inbred rat strains. ④DNA fingerprinting of filial generation which RCS and LEW hybridized is analyzed, whose bands are found in their parents bands and complied with Mendel heredity law. (2) Microsatellite DNA:①DNA polymorphism was studied using microsatellite DNA method in 6 inbred rat strains. There is much polymorphism among the various rat strains;It was coincidence among the same inbred strains rat groups. ②10 microsatellite loci are analyzed in 6 inbred rat strains group. and the similarity c展开更多
文摘目的通过膀胱灌注N蛳甲基亚硝基脲(M N U)诱导近交系大鼠膀胱肿瘤。方法选取F344大鼠20只,膀胱灌注M N U,每2周1次,共5次;于第14周处死动物,取膀胱肿瘤组织测M H C蛳I及病理观察。结果M N U组肿瘤发生率为100%;膀胱肿瘤的M H C蛳I表达较高(58.60%￣93.80%)。结论M N U可成功诱导F344大鼠膀胱肿瘤的形成,是肿瘤免疫治疗的理想模型。
文摘Monitoring method of biochemical markers is inferred corresponding gene changed according to protein changed in laboratory animals, and have definite limitation in some extent. But DNA fingerprints and microsatellite DNA directly monitored DNA of genetic materials, more accurate、more reliable and direct than biochemical markers. In the experiments six domestic strains(SHR、SHR/sp、WKY、LEW、RCS、F344) of inbred rat were analyzed DNA polymorphism using DNA fingerprints and microsatellite DNA, and set up the exact、reliable、speedy、convenient method of in the heredity monitoring of inbred rats. The result indicated:(1)DNA fingerprints: ①There were 14-19 distinguishable bands on 4.0kb-23.1kb every DNA fingerprinting. The similarity coefficient(F) and the probability of band sharing(X) were more than 0.9 in different individual of the identical inbred rat strain. and indicated more high similarity in genetic materials of the various individual in same group rats. ②The similarity coefficient and the probability of band sharing of DNA fingerprinting were less than 0.07, probability of the identical DNA fingerprinting was less than 10 -22 among the different individuals of different inbred strain. and indicated that there was more different in genetic materials of various individual in different inbred rat groups. ③)DNA fingerprinting obtained by four times experiment from same individual genome are almost coincident DNA fingerprinting from various tissues are also coincident in the same individual of 6 inbred rat strains. ④DNA fingerprinting of filial generation which RCS and LEW hybridized is analyzed, whose bands are found in their parents bands and complied with Mendel heredity law. (2) Microsatellite DNA:①DNA polymorphism was studied using microsatellite DNA method in 6 inbred rat strains. There is much polymorphism among the various rat strains;It was coincidence among the same inbred strains rat groups. ②10 microsatellite loci are analyzed in 6 inbred rat strains group. and the similarity c
