Objective： The aim of the study was to investigate whether enhanced anti-tumor effects of endostar （ES） on radiation involved hypoxia inducible factor-la （HIF-la） and basic ~broblast growth factor （bFGF）. Meth...Objective： The aim of the study was to investigate whether enhanced anti-tumor effects of endostar （ES） on radiation involved hypoxia inducible factor-la （HIF-la） and basic ~broblast growth factor （bFGF）. Methods： A549 cells were divided into four groups： control group; endostar （ES） group; radiation （RT） group; endostar plus radiation （ES ＋ RT） group. The inhibition of proliferation rates ofA549 cells was measured by cell counting kit-8 （CCK-8）. HIF-la and bFGF expression levels were checked by enzyme linked immunosorbent assay （ELISA） and reverse transcription-polymerase chain reaction （RT-PCR）. Results： The proliferation inhibition rate in the ES ＋ RT group was higher than that in other groups. On the other hand, the expressions of HIF-1α and bFGF in the ES ＋ RT group were significantly reduced compared to other groups. HIF-1α and bFGF levels were positively correlated （r = 0.80, P 〈 0.01）. Conclusion： Our results suggest that endostar could enhance anti-tumor effect of radiation by reducing expressions of HIF-1α and bFGF.展开更多
文摘Objective： The aim of the study was to investigate whether enhanced anti-tumor effects of endostar （ES） on radiation involved hypoxia inducible factor-la （HIF-la） and basic ~broblast growth factor （bFGF）. Methods： A549 cells were divided into four groups： control group; endostar （ES） group; radiation （RT） group; endostar plus radiation （ES ＋ RT） group. The inhibition of proliferation rates ofA549 cells was measured by cell counting kit-8 （CCK-8）. HIF-la and bFGF expression levels were checked by enzyme linked immunosorbent assay （ELISA） and reverse transcription-polymerase chain reaction （RT-PCR）. Results： The proliferation inhibition rate in the ES ＋ RT group was higher than that in other groups. On the other hand, the expressions of HIF-1α and bFGF in the ES ＋ RT group were significantly reduced compared to other groups. HIF-1α and bFGF levels were positively correlated （r = 0.80, P 〈 0.01）. Conclusion： Our results suggest that endostar could enhance anti-tumor effect of radiation by reducing expressions of HIF-1α and bFGF.
