近年多把脑外伤区分为局部脑损伤和弥漫性脑损伤二大类。弥漫性轴突损伤(diffuse axonalinjury,DAI)属重度弥漫性脑损伤。DAI的概念最初来自Strich对5例弥漫性重度脑白质变性(diffuse severe degeneration of the white matter)的报告...近年多把脑外伤区分为局部脑损伤和弥漫性脑损伤二大类。弥漫性轴突损伤(diffuse axonalinjury,DAI)属重度弥漫性脑损伤。DAI的概念最初来自Strich对5例弥漫性重度脑白质变性(diffuse severe degeneration of the white matter)的报告中。同义词尚有剪力伤(shearing injury)、弥漫性脑损害(diffuse braia demage)、中央性大脑创伤(central cerebral trauma)等。Adams、Gennarelli等对此作了详细研究。展开更多
OBJECTIVE: To evaluate the effect of Tanreqing injection on axon myelin in the mouse brain of experimental autoimmune encephalomyelitis(EAE).METHODS: An EAE model was established by myelin oligodendrocyte glycoprotein...OBJECTIVE: To evaluate the effect of Tanreqing injection on axon myelin in the mouse brain of experimental autoimmune encephalomyelitis(EAE).METHODS: An EAE model was established by myelin oligodendrocyte glycoprotein(MOG)35-55 immunization in C57BL/6 mice. Mice were randomly divided into the following groups: normal, model,prednisone acetate(PA)(6 mg/kg), Tanreqing high dose(5.14 m L/kg), Tanreqing low dose(2.57 m L/kg). On the day of immunization, both Tanreqing groups were treated by intraperitoneal injection,with the PA group treated by intragastrical perfusion after T cell response, and the other groups treated with saline. Changes in body weight, neurological deficit score, incidence rate, mortality rate,and course of disease were observed for all mice.Brain tissue was isolated and stained with hematoxylin-eosin, and pathological investigations performed to evaluate axon myelin damage by transmission electron microscopy(TEM). Myelin basic protein and microtubule associated protein-2 were analyzed by immunohistochemistry.RESULTS: Tanreqing injection significantly prolonged EAE latency and decreased the neurological deficit score, alleviated infiltration of inflammatory cells in the focus area, up-regulated hippocampal MBP expression at the acute stage and the remission stage, and increased microtubule associated protein-2 expression in the EAE brain to varying degrees in the acute stage. TEM analysis indicated that Tanreqing injection alleviates myelin damage in the EAE mouse and maintains the integrity of circular layer structures and alleviates axon mitochondrial swelling.CONCLUSION: Tanreqing injection alleviates EAE symptoms.展开更多
目的：建立乳鼠皮层神经元体外培养模型，观察氧糖剥夺处理对神经元轴突损伤的影响。方法取出生24 h 内的 SD 大鼠大脑皮层神经元，采用逐渐降低培养液中血清浓度和添加阿糖胞苷的方法培养、纯化神经元，观察其形态并鉴定。建立氧糖剥...目的：建立乳鼠皮层神经元体外培养模型，观察氧糖剥夺处理对神经元轴突损伤的影响。方法取出生24 h 内的 SD 大鼠大脑皮层神经元，采用逐渐降低培养液中血清浓度和添加阿糖胞苷的方法培养、纯化神经元，观察其形态并鉴定。建立氧糖剥夺模型，随机分为正常对照组和氧糖剥夺处理组（2、4、6、8 h 各1组）。采用噻唑蓝比色法测定神经元活性，βⅢ-tubulin 免疫荧光染色观察轴突形态学变化。结果神经元培养3～5 d 后，胞体变得饱满并分出轴突和树突，突起之间逐渐交织成密集网络，神经元微管相关蛋白-2（MAP-2）标记阳性率＞95％。与正常对照组相比，随着氧糖剥夺时间的延长，轴突网络逐渐损伤、崩解，同时神经元活性呈梯度下降（P ＜0.01）。结论该研究成功建立了神经元体外原代培养及氧糖剥夺模型，并观察到在氧糖剥夺处理过程中神经元的轴突损伤及同时出现的神经元活性下降。展开更多
文摘近年多把脑外伤区分为局部脑损伤和弥漫性脑损伤二大类。弥漫性轴突损伤(diffuse axonalinjury,DAI)属重度弥漫性脑损伤。DAI的概念最初来自Strich对5例弥漫性重度脑白质变性(diffuse severe degeneration of the white matter)的报告中。同义词尚有剪力伤(shearing injury)、弥漫性脑损害(diffuse braia demage)、中央性大脑创伤(central cerebral trauma)等。Adams、Gennarelli等对此作了详细研究。
基金Supported by the Research on the Effect of Catalpol on the OPCs' Proliferation and Differentiation(No.81173237)Research on the Impact of OPCs and Remyelination via the Method of Bushenyisu with EAE mice(No.81072765)of National Natural Science Foundation
文摘OBJECTIVE: To evaluate the effect of Tanreqing injection on axon myelin in the mouse brain of experimental autoimmune encephalomyelitis(EAE).METHODS: An EAE model was established by myelin oligodendrocyte glycoprotein(MOG)35-55 immunization in C57BL/6 mice. Mice were randomly divided into the following groups: normal, model,prednisone acetate(PA)(6 mg/kg), Tanreqing high dose(5.14 m L/kg), Tanreqing low dose(2.57 m L/kg). On the day of immunization, both Tanreqing groups were treated by intraperitoneal injection,with the PA group treated by intragastrical perfusion after T cell response, and the other groups treated with saline. Changes in body weight, neurological deficit score, incidence rate, mortality rate,and course of disease were observed for all mice.Brain tissue was isolated and stained with hematoxylin-eosin, and pathological investigations performed to evaluate axon myelin damage by transmission electron microscopy(TEM). Myelin basic protein and microtubule associated protein-2 were analyzed by immunohistochemistry.RESULTS: Tanreqing injection significantly prolonged EAE latency and decreased the neurological deficit score, alleviated infiltration of inflammatory cells in the focus area, up-regulated hippocampal MBP expression at the acute stage and the remission stage, and increased microtubule associated protein-2 expression in the EAE brain to varying degrees in the acute stage. TEM analysis indicated that Tanreqing injection alleviates myelin damage in the EAE mouse and maintains the integrity of circular layer structures and alleviates axon mitochondrial swelling.CONCLUSION: Tanreqing injection alleviates EAE symptoms.
文摘目的：建立乳鼠皮层神经元体外培养模型，观察氧糖剥夺处理对神经元轴突损伤的影响。方法取出生24 h 内的 SD 大鼠大脑皮层神经元，采用逐渐降低培养液中血清浓度和添加阿糖胞苷的方法培养、纯化神经元，观察其形态并鉴定。建立氧糖剥夺模型，随机分为正常对照组和氧糖剥夺处理组（2、4、6、8 h 各1组）。采用噻唑蓝比色法测定神经元活性，βⅢ-tubulin 免疫荧光染色观察轴突形态学变化。结果神经元培养3～5 d 后，胞体变得饱满并分出轴突和树突，突起之间逐渐交织成密集网络，神经元微管相关蛋白-2（MAP-2）标记阳性率＞95％。与正常对照组相比，随着氧糖剥夺时间的延长，轴突网络逐渐损伤、崩解，同时神经元活性呈梯度下降（P ＜0.01）。结论该研究成功建立了神经元体外原代培养及氧糖剥夺模型，并观察到在氧糖剥夺处理过程中神经元的轴突损伤及同时出现的神经元活性下降。
