目的:评估以胶原、羟基磷灰石、硫酸软骨素等3种天然骨骼基本成分构建成的三维多孔骨修复材料的理化性能和体内生物学性能,观察其作为骨形态发生蛋白(bone morphogenetic protein 2,BMP-2)载体的效果。方法:以胶原、羟基磷灰石、硫酸软...目的:评估以胶原、羟基磷灰石、硫酸软骨素等3种天然骨骼基本成分构建成的三维多孔骨修复材料的理化性能和体内生物学性能,观察其作为骨形态发生蛋白(bone morphogenetic protein 2,BMP-2)载体的效果。方法:以胶原、羟基磷灰石、硫酸软骨素及BMP-2为原料,通过化学交联和冷冻干燥的方法构建具有一定三维结构的骨修复材料。通过HE染色、扫描电镜观察材料的结构性能;通过表面能谱、X线衍射观察材料的理化性能;将骨髓基质干细胞(marrow stromal cells,MSCs)种植在材料表面,观察MSCs在材料表面的粘附、增生和分化;将该复合材料种植在大鼠体内,观察材料在体内的降解和异位成骨情况。结果:骨修复材料在植入局部保持完整的支架结构,具有利于细胞粘附和增殖的多孔结构。通过肌肉埋植,在异位诱导形成了骨组织,并且随着骨组织的形成,支架逐渐降解吸收。结论:胶原-羟基磷灰石-硫酸软骨素-骨形态发生蛋白是具有良好的生物相容性和骨诱导特性的骨修复材料。展开更多
Purpose:To investigate the mechanism of the termination of ocular dominance column plasticity by electrophysiologic analysis and 2-dimensional electrophoresis-mass spectrography(2-DE/MS).Methods:The changes in ocular ...Purpose:To investigate the mechanism of the termination of ocular dominance column plasticity by electrophysiologic analysis and 2-dimensional electrophoresis-mass spectrography(2-DE/MS).Methods:The changes in ocular dominance columns following monocular deprivation were electrophysiologically detected in 22-day-old,100-day-old and chondroitinase-perfused 100-day-old rats.Total protein of grey matter of the primary visual cortex was extracted and studied by 2-DE/MS from the three groups of rats.Results:Monocular deprivation may lead to shifts in ocular dominance columns in 22-day-old and chondroitinase-perfused 100-day-old rats,but not in 100-day-old rats.Four protein spots present in grey matter of the primary visual cortex in 100-day-old,but not in that of 22-day-old and chondroitinase-perfused rats,and mass spectrography identified two of these proteins.Conclusions:The electrophysiologic results show that ocular dominance column plasticity presents in 22-day-old rats,ends up in 100-day-old rats and restored in chondroitinase-perfused 100-day-old rats.2-DE/MS results show that phosphatidylethanolamine binding protein and glial fibrillary acidic protein delta may be associated with the termination of ocular dominance column plasticity in the rat,but need more evidence to confirm it.展开更多
文摘Purpose:To investigate the mechanism of the termination of ocular dominance column plasticity by electrophysiologic analysis and 2-dimensional electrophoresis-mass spectrography(2-DE/MS).Methods:The changes in ocular dominance columns following monocular deprivation were electrophysiologically detected in 22-day-old,100-day-old and chondroitinase-perfused 100-day-old rats.Total protein of grey matter of the primary visual cortex was extracted and studied by 2-DE/MS from the three groups of rats.Results:Monocular deprivation may lead to shifts in ocular dominance columns in 22-day-old and chondroitinase-perfused 100-day-old rats,but not in 100-day-old rats.Four protein spots present in grey matter of the primary visual cortex in 100-day-old,but not in that of 22-day-old and chondroitinase-perfused rats,and mass spectrography identified two of these proteins.Conclusions:The electrophysiologic results show that ocular dominance column plasticity presents in 22-day-old rats,ends up in 100-day-old rats and restored in chondroitinase-perfused 100-day-old rats.2-DE/MS results show that phosphatidylethanolamine binding protein and glial fibrillary acidic protein delta may be associated with the termination of ocular dominance column plasticity in the rat,but need more evidence to confirm it.
