Production of lipase by Rhizopus arrhizus was studied in a rotating biological contactor(RBC)with polyurethane foam(PUF),as a porous biomass support,attached on both sides of the RBC disks.The effects of aeration rate...Production of lipase by Rhizopus arrhizus was studied in a rotating biological contactor(RBC)with polyurethane foam(PUF),as a porous biomass support,attached on both sides of the RBC disks.The effects of aeration rate,rotating rate of discs,volume of broth and the number of discs on the lipase activity were studied in the 6 L RBC,and the optimum fermentation condition was as follows:4 L·min-1 aeration rate,100 r·min-1 rotating rate,3.5 L medium with three discs in 6 L RBC.Repeated batch fermentation by immobilized mycelium was tested and it was found that immobilized cells showed high stability for repeated use.Five repeated batches could be carried out in 6 L RBC and the lipase productivity increased from 3125 U·h-1 in batch fermentation to 9512 U·h-1 in repeated batch fermentation,which was 3 times as high as that in batch fermentation.展开更多
最近，英国巴斯大学（University of Bath）的研究人员开发出一种新型的酶催化过程强化技术——旋转纺布盘反应器（SCDR）。这种技术是当前仅有的少数几个可以加快酶反应的技术之一。SCDR原理很简单，基于常规的旋转盘反应器（SDR）原...最近，英国巴斯大学（University of Bath）的研究人员开发出一种新型的酶催化过程强化技术——旋转纺布盘反应器（SCDR）。这种技术是当前仅有的少数几个可以加快酶反应的技术之一。SCDR原理很简单，基于常规的旋转盘反应器（SDR）原理，但又有所创新。SCDR利用离心力使薄膜在覆盖着固定了酶的纺布的旋转圆盘上伸展。展开更多
文摘Production of lipase by Rhizopus arrhizus was studied in a rotating biological contactor(RBC)with polyurethane foam(PUF),as a porous biomass support,attached on both sides of the RBC disks.The effects of aeration rate,rotating rate of discs,volume of broth and the number of discs on the lipase activity were studied in the 6 L RBC,and the optimum fermentation condition was as follows:4 L·min-1 aeration rate,100 r·min-1 rotating rate,3.5 L medium with three discs in 6 L RBC.Repeated batch fermentation by immobilized mycelium was tested and it was found that immobilized cells showed high stability for repeated use.Five repeated batches could be carried out in 6 L RBC and the lipase productivity increased from 3125 U·h-1 in batch fermentation to 9512 U·h-1 in repeated batch fermentation,which was 3 times as high as that in batch fermentation.
文摘最近，英国巴斯大学（University of Bath）的研究人员开发出一种新型的酶催化过程强化技术——旋转纺布盘反应器（SCDR）。这种技术是当前仅有的少数几个可以加快酶反应的技术之一。SCDR原理很简单，基于常规的旋转盘反应器（SDR）原理，但又有所创新。SCDR利用离心力使薄膜在覆盖着固定了酶的纺布的旋转圆盘上伸展。
