为快速定量大麦贮藏蛋白基因B-hordein差异表达,利用SYBR Green I染料法测定转基因大麦和对照花后10 d籽粒B-hordein表达量,通过RT-PCR扩增B-hordein和内参基因actin片段,对PCR退火温度、引物浓度等进行优化,结合扩增曲线和熔解曲线分...为快速定量大麦贮藏蛋白基因B-hordein差异表达,利用SYBR Green I染料法测定转基因大麦和对照花后10 d籽粒B-hordein表达量,通过RT-PCR扩增B-hordein和内参基因actin片段,对PCR退火温度、引物浓度等进行优化,结合扩增曲线和熔解曲线分析。结果表明,转基因大麦B-hordein基因表达量较对照品种Golden Promise显著降低(P<0.05),B-hordein和actin扩增片段分别在(84.51±0.01)℃和(80±0.01)℃处显示特异性单峰,可成功建立转基因大麦B-hordein SYBR Green I实时定量PCR法。展开更多
美国欧塞奇生物能源公司（Osage Bio Energy）用大麦乙醇生产的副产物生产大麦蛋白粉（BPM）作为一种新的富含蛋白质的动物饲料。BPM低脂肪，高蛋白，赖氨酸含量与其它谷物饲料相当。此外，这种产品源于非转基因大麦，为全球的客户提供...美国欧塞奇生物能源公司（Osage Bio Energy）用大麦乙醇生产的副产物生产大麦蛋白粉（BPM）作为一种新的富含蛋白质的动物饲料。BPM低脂肪，高蛋白，赖氨酸含量与其它谷物饲料相当。此外，这种产品源于非转基因大麦，为全球的客户提供了一种新的非转基因动物饲料来源。托克斯生物能源公司（Appomattox Bio Energy）每年BPM的生产能力可以达到25万吨。欧塞奇生物能源公司设计了创新的生产工艺流程，通过特殊的碾磨技术除去大麦壳减少纤维，使蛋白质和淀粉浓缩，除去的大麦壳制粒后可作为再生能源。展开更多
A transgenic barley line(LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene(PTrx) was employed to measure the growth,protein oxidation,cell viability,and antioxidase activity in barley roots during ...A transgenic barley line(LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene(PTrx) was employed to measure the growth,protein oxidation,cell viability,and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl3 on filter paper.The results show that(1) compared with the non-transgenic barley,LSY-11-1-1 had enhanced root growth,although both were seriously inhibited after AlCl3 treatment;(2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley,as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1;(3) activities of catalase(CAT) ,glutathione peroxidase(GPX) ,ascorbate peroxidase(APX) ,and glu-tathione reductase(GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips,although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress.These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity,thereby quenching ROS caused by AlCl3 during germination.These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.展开更多
文摘为快速定量大麦贮藏蛋白基因B-hordein差异表达,利用SYBR Green I染料法测定转基因大麦和对照花后10 d籽粒B-hordein表达量,通过RT-PCR扩增B-hordein和内参基因actin片段,对PCR退火温度、引物浓度等进行优化,结合扩增曲线和熔解曲线分析。结果表明,转基因大麦B-hordein基因表达量较对照品种Golden Promise显著降低(P<0.05),B-hordein和actin扩增片段分别在(84.51±0.01)℃和(80±0.01)℃处显示特异性单峰,可成功建立转基因大麦B-hordein SYBR Green I实时定量PCR法。
文摘美国欧塞奇生物能源公司（Osage Bio Energy）用大麦乙醇生产的副产物生产大麦蛋白粉（BPM）作为一种新的富含蛋白质的动物饲料。BPM低脂肪，高蛋白，赖氨酸含量与其它谷物饲料相当。此外，这种产品源于非转基因大麦，为全球的客户提供了一种新的非转基因动物饲料来源。托克斯生物能源公司（Appomattox Bio Energy）每年BPM的生产能力可以达到25万吨。欧塞奇生物能源公司设计了创新的生产工艺流程，通过特殊的碾磨技术除去大麦壳减少纤维，使蛋白质和淀粉浓缩，除去的大麦壳制粒后可作为再生能源。
基金Project supported by the National Natural Science Foundation of China(No.30871530)the Science and Technology Transformation Plan of Henan Province(No.0636000005)+3 种基金One Hundred-Talent Plan of Chinese Academy of Sciences(CAS)CAS-local Government Cooperative Projectthe CAS/SAFEA International Partnership Program for Creative Research TeamsCAS Young Scientists Fellowship(No.2009Y2B211),China
文摘A transgenic barley line(LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene(PTrx) was employed to measure the growth,protein oxidation,cell viability,and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl3 on filter paper.The results show that(1) compared with the non-transgenic barley,LSY-11-1-1 had enhanced root growth,although both were seriously inhibited after AlCl3 treatment;(2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley,as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1;(3) activities of catalase(CAT) ,glutathione peroxidase(GPX) ,ascorbate peroxidase(APX) ,and glu-tathione reductase(GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips,although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress.These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity,thereby quenching ROS caused by AlCl3 during germination.These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.
