Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine.Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups（n=6 in each group）: groupⅠ received saline ...Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine.Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups（n=6 in each group）: groupⅠ received saline i.v.;group Ⅱ received glutamine（Gln） 0.15 g·kg-1 i.v.;group Ш received Gln 0.45 g·kg-1 i.v.;group Ⅳ received Gln 0.75 g·kg-1 i.v..All rats were killed after 4 h administration of Gln.HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.② Thirty healthy Sprague-Dawley rats were randomly divided into five groups（n=6 in each group）.They were killed at 1 h（group 1 h）、6 h（group 6 h）、12 h（group 12 h）、24 h（group 24 h） and 48 h（group 48 h） after administration of Gln(0.75 g·kg-1).HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results ① The expression of HSP70 mRNA in group Ⅱ、Ш and Ⅳ were higher than groupⅠ（P<0.05）,and the expression in groupⅡ、Ш and Ⅳ increased gradually.②The expression of HSP70 mRNA in group 1 h、 6 h and 12 h was higher than that in 24 h and 48h（P<0.05）.Conclusion Glutamine induces the expression of HSP70 mRNA in rats,and it occurs with the manner of dose and time dependent.展开更多
AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental rats.METHODS:All rats were randomly divided into normal control group,model control group t...AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental rats.METHODS:All rats were randomly divided into normal control group,model control group treated with CCl4 for 12 wk,CCl4+NAC group treated with CCl4+NAC(100 mg/kg,i.p.)for 12 wk,CCl4+MEL-1 group treated with CCl4+melatonin(2.5 mg/kg)for 12 wk,CCl4+MEL-2 group treated with CCl4+ melatonin(5.0 mg/kg)for 12 wk,and CCl4+MEL-3 group treated with CCl4+melatonin(10 mg/kg).Rats in the treatment groups were injected subcutaneously with sterile CCl4(3 mL/kg,body weight)in a ratio of 2:3 with olive oil twice a week.Rats in normal control group received hypodermic injection of olive oil at the same dose and frequency as those in treatment groups.At the end of experiment,rats in each group were anesthetized and sacrificed.Hematoxylin and eosin(HE)staining and Van Gieson staining were used to examine changes in liver pathology.Serum activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and protein concentration weremeasured with routine laboratory methods using an autoanalyzer.Hydroxyproline(HYP)content in liver and malondialdehyde(MDA)and glutathione peroxidase(GPx)levels in liver homogenates were assayed by spectrophotometry.Serum hyaluronic acid(HA),laminin(LN),and procollagenⅢN-terminal peptide(PⅢNP)were determined by radioimmunoassay.RESULTS:Pathologic grading showed that the fibrogenesis was much less severe in CCl4+MEL3 group than in model control group(u=2.172,P<0.05),indicating that melatonin(10 mg/kg)can significantly ameliorate CCl4-induced hepatic fibrotic changes.The serum levels of ALT and AST were markedly lower in CCl4+MEL treatment groups(5,10 mg/kg)than in model control group(ALT:286.23 ±121.91 U/L vs 201.15±101.16 U/L and 178.67 ±103.14 U/L,P=0.028,P=0.007;AST:431.00 ±166.35 U/L vs 321.23±162.48 U/L and 292.42 ±126.23 U/L,P=0.043,P=0.013).Similarly,the serum laminin(LN)and hyaluronic acid(HA)levels and hydroxyproline(HYP)contents in liver were signific展开更多
文摘Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine.Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups（n=6 in each group）: groupⅠ received saline i.v.;group Ⅱ received glutamine（Gln） 0.15 g·kg-1 i.v.;group Ш received Gln 0.45 g·kg-1 i.v.;group Ⅳ received Gln 0.75 g·kg-1 i.v..All rats were killed after 4 h administration of Gln.HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.② Thirty healthy Sprague-Dawley rats were randomly divided into five groups（n=6 in each group）.They were killed at 1 h（group 1 h）、6 h（group 6 h）、12 h（group 12 h）、24 h（group 24 h） and 48 h（group 48 h） after administration of Gln(0.75 g·kg-1).HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results ① The expression of HSP70 mRNA in group Ⅱ、Ш and Ⅳ were higher than groupⅠ（P<0.05）,and the expression in groupⅡ、Ш and Ⅳ increased gradually.②The expression of HSP70 mRNA in group 1 h、 6 h and 12 h was higher than that in 24 h and 48h（P<0.05）.Conclusion Glutamine induces the expression of HSP70 mRNA in rats,and it occurs with the manner of dose and time dependent.
基金Supported by The Natural Science Foundation of Anhui Province No.01043904the Natural Science Research Project of Colleges and Universities of Anhui Province No.KJ2007B146
文摘AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental rats.METHODS:All rats were randomly divided into normal control group,model control group treated with CCl4 for 12 wk,CCl4+NAC group treated with CCl4+NAC(100 mg/kg,i.p.)for 12 wk,CCl4+MEL-1 group treated with CCl4+melatonin(2.5 mg/kg)for 12 wk,CCl4+MEL-2 group treated with CCl4+ melatonin(5.0 mg/kg)for 12 wk,and CCl4+MEL-3 group treated with CCl4+melatonin(10 mg/kg).Rats in the treatment groups were injected subcutaneously with sterile CCl4(3 mL/kg,body weight)in a ratio of 2:3 with olive oil twice a week.Rats in normal control group received hypodermic injection of olive oil at the same dose and frequency as those in treatment groups.At the end of experiment,rats in each group were anesthetized and sacrificed.Hematoxylin and eosin(HE)staining and Van Gieson staining were used to examine changes in liver pathology.Serum activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and protein concentration weremeasured with routine laboratory methods using an autoanalyzer.Hydroxyproline(HYP)content in liver and malondialdehyde(MDA)and glutathione peroxidase(GPx)levels in liver homogenates were assayed by spectrophotometry.Serum hyaluronic acid(HA),laminin(LN),and procollagenⅢN-terminal peptide(PⅢNP)were determined by radioimmunoassay.RESULTS:Pathologic grading showed that the fibrogenesis was much less severe in CCl4+MEL3 group than in model control group(u=2.172,P<0.05),indicating that melatonin(10 mg/kg)can significantly ameliorate CCl4-induced hepatic fibrotic changes.The serum levels of ALT and AST were markedly lower in CCl4+MEL treatment groups(5,10 mg/kg)than in model control group(ALT:286.23 ±121.91 U/L vs 201.15±101.16 U/L and 178.67 ±103.14 U/L,P=0.028,P=0.007;AST:431.00 ±166.35 U/L vs 321.23±162.48 U/L and 292.42 ±126.23 U/L,P=0.043,P=0.013).Similarly,the serum laminin(LN)and hyaluronic acid(HA)levels and hydroxyproline(HYP)contents in liver were signific
