AIM:To observe synthesis of CD14 protein and expressionof CD14 mRNA in hepatic tissue and hepatocytes of ratsduring endotoxemia.METHODS:The endotoxemia model of Wistar rat wasestablished by injection of a dose of lipo...AIM:To observe synthesis of CD14 protein and expressionof CD14 mRNA in hepatic tissue and hepatocytes of ratsduring endotoxemia.METHODS:The endotoxemia model of Wistar rat wasestablished by injection of a dose of lipopolysaccharide(LPS)(5mg·kg^(-1),Escherichia coil O111:B4)via the tailvein,then the rats were sacrificed after 3,6,12 and 24 h inbtaches.Hepatocytes were isolated from normal and LPS-injected rats by in situ collagenase perfusion technique andwere collected to measure the expression of CD14 mRNAand synthesis of CD14 protein by reverse transcription-polymerase chain reaction(RT-PCR)or Western blotanalysis.The binding of fluorescein isothiecyanate(FITC)-CD14 polyclonal antibody to isolated hepatocytes was alsoassessed by flow cytometric analysis(FCM).RESULTS:In the rats with endotoxemia,the expressions ofCD14 mRNA in hepatic tissue and isolated hepatocytes werestronger at 3,6,and 12 h than that in control rats(3.48±0.15,5.89±0.62,4.33±0.18,vs 1.35±0.14 in hepatictissue,P<0.01;4.12±0.17,6.24±0.64,4.35±0.18,vs1.87±0.15 in hepatecytss,P<0.01).The synthesis of CD14protein in hepatic tissue and isolated hepatoeytes increasesalso obviously in 6 and 12 h when compared to that incontrol rats(13.27±1.27,17.32±1.35,11.42±1.20,vs 7.34±0.72 in hepatic tissue,P<0.01;14.68±_+1.30,17.95±1.34,11.65±1.19,vs 7.91±0.70 in hepatocytoes,P<0.01).FCM showed that mean fluorescence intensity(MFI)andnumbers of FITC-CD14 positive cells in the rats withendotoxemia increased obviously at 3,6,12 and 24h whencompared with normal control group(43.4%,70.2%,91.4%,32.6% vs4.5%,P<0.01).CONCLUSION:LPS can markedly promote the synthesis ofCD]4 protein and up-regulate the expression of CD14 mRNAin isolated hepatocytes and hepatic tissue.Liver might be amain source for soluble CD14 production duringendotoxemia.展开更多
We report a new strategy to prepare gold nanoflowers （AuNFs） using a two-step seed-mediated method. The as-prepared AuNFs were employed as surface-enhance Raman scattering （SERS） substrates, showing strong signal ...We report a new strategy to prepare gold nanoflowers （AuNFs） using a two-step seed-mediated method. The as-prepared AuNFs were employed as surface-enhance Raman scattering （SERS） substrates, showing strong signal enhancement. We further found that iodide ions （I^-） could selectively induce the morphological transformation of AuNFs to spheres, resulting in a blue-shift of the localized surface plasmon resonance （LSPR） bands, a color change of the AuNFs solution from blue to red, and decreased SERS activity. This behavior allows the AuNFs to be used in the determination of I^-.展开更多
基金the National Natural Science Foundation of China(No.39970719)
文摘AIM:To observe synthesis of CD14 protein and expressionof CD14 mRNA in hepatic tissue and hepatocytes of ratsduring endotoxemia.METHODS:The endotoxemia model of Wistar rat wasestablished by injection of a dose of lipopolysaccharide(LPS)(5mg·kg^(-1),Escherichia coil O111:B4)via the tailvein,then the rats were sacrificed after 3,6,12 and 24 h inbtaches.Hepatocytes were isolated from normal and LPS-injected rats by in situ collagenase perfusion technique andwere collected to measure the expression of CD14 mRNAand synthesis of CD14 protein by reverse transcription-polymerase chain reaction(RT-PCR)or Western blotanalysis.The binding of fluorescein isothiecyanate(FITC)-CD14 polyclonal antibody to isolated hepatocytes was alsoassessed by flow cytometric analysis(FCM).RESULTS:In the rats with endotoxemia,the expressions ofCD14 mRNA in hepatic tissue and isolated hepatocytes werestronger at 3,6,and 12 h than that in control rats(3.48±0.15,5.89±0.62,4.33±0.18,vs 1.35±0.14 in hepatictissue,P<0.01;4.12±0.17,6.24±0.64,4.35±0.18,vs1.87±0.15 in hepatecytss,P<0.01).The synthesis of CD14protein in hepatic tissue and isolated hepatoeytes increasesalso obviously in 6 and 12 h when compared to that incontrol rats(13.27±1.27,17.32±1.35,11.42±1.20,vs 7.34±0.72 in hepatic tissue,P<0.01;14.68±_+1.30,17.95±1.34,11.65±1.19,vs 7.91±0.70 in hepatocytoes,P<0.01).FCM showed that mean fluorescence intensity(MFI)andnumbers of FITC-CD14 positive cells in the rats withendotoxemia increased obviously at 3,6,12 and 24h whencompared with normal control group(43.4%,70.2%,91.4%,32.6% vs4.5%,P<0.01).CONCLUSION:LPS can markedly promote the synthesis ofCD]4 protein and up-regulate the expression of CD14 mRNAin isolated hepatocytes and hepatic tissue.Liver might be amain source for soluble CD14 production duringendotoxemia.
基金supported by the National Natural Science Foundation of China (21305113)the Chongqing Fundamental and Advanced Research Project (cstc2013jcyjA50008)+2 种基金the Fundamental Research Funds for the Central Universities (XDJK2015B029)the fund of State Key Laboratory of Electroanalytical Chemistry (Changchun Institute of Applied Chemistry, Chinese Academy of Sciences) (SKLEAC201312)the Research Fund for the Doctor Program of Southwest University (swu111077)
文摘We report a new strategy to prepare gold nanoflowers （AuNFs） using a two-step seed-mediated method. The as-prepared AuNFs were employed as surface-enhance Raman scattering （SERS） substrates, showing strong signal enhancement. We further found that iodide ions （I^-） could selectively induce the morphological transformation of AuNFs to spheres, resulting in a blue-shift of the localized surface plasmon resonance （LSPR） bands, a color change of the AuNFs solution from blue to red, and decreased SERS activity. This behavior allows the AuNFs to be used in the determination of I^-.
