【目的】筛选获得印度洋海水解有机磷细菌,研究其解有机磷作用机理,初步了解该地区可培养有机磷细菌的系统发育多样性。【方法】采用卵磷脂培养基从分离自印度洋的细菌中筛选解有机磷细菌,根据16S r RNA基因序列确定获得的有机磷细菌的...【目的】筛选获得印度洋海水解有机磷细菌,研究其解有机磷作用机理,初步了解该地区可培养有机磷细菌的系统发育多样性。【方法】采用卵磷脂培养基从分离自印度洋的细菌中筛选解有机磷细菌,根据16S r RNA基因序列确定获得的有机磷细菌的分类地位;同时挑选解磷效果显著的3株细菌利用液体发酵进行产酶和解磷特性分析。【结果】自916株细菌中得到99株具有解有机磷活性的细菌,分属于16个属。在以卵磷脂为其有机磷来源时,菌株India-BSP-1(Cobetia sp.)、India-BSP-21(Pelagibaca sp.)、India-BSP-23(Pelagibacterium sp.)的培养液中磷酸盐(DIP)浓度曲线为N型,并且碱性磷酸酶的检测活性滞后于DIP的生成。【结论】印度洋海水中解有机磷细菌种类多样性丰富,疑似有新种;解有机磷细菌的解磷特性受DIP和碱性磷酸酶相互作用的影响。展开更多
In order to obtain high-efficiency organophosphorus solubilizing bacteria, 21 strains of organophosphate solubilizing bacteria were isolated from the rhizosphere soil of Camellia oleifera plants, and the transparent c...In order to obtain high-efficiency organophosphorus solubilizing bacteria, 21 strains of organophosphate solubilizing bacteria were isolated from the rhizosphere soil of Camellia oleifera plants, and the transparent circle method was used for rescreening. Only 4 strains of bacteria could form transparent circle on organophosphorus medium. The D/d value of 4 strains of bacteria was between 1.62 and 2.71, among which the D/d value of strain Y6 was the highest(2.71). The available phosphorus content of the fermentation supernatant was 8.50~14.79 mg/L, which was 7.88~14.17 mg/L higher than that of CK. The strain Y6 had the highest soluble phosphorus content of 14.79 mg/L in the fermentation supernatant, which was 14.17 mg/L higher than that of CK. According to the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis of strain Y6, it is preliminarily determined that strain Y6 is Pseudomonas. Strain Y6 is beneficial to improve the supply of phosphorus in rhizosphere soil of Camellia oleifera and promote the growth of Camellia oleifera. It has great potential in the development of bio-organic fertilizer.展开更多
基金湖南省科技厅重点研发计划(2017N K 2144)长沙市科技局重大专项(kq1804016)+1 种基金湖南农业科技创新联盟项目(2017LM0305)重金属污染耕地安全高效利用湖南省工程研究中心开放研究基金(TGOP-001)。
文摘In order to obtain high-efficiency organophosphorus solubilizing bacteria, 21 strains of organophosphate solubilizing bacteria were isolated from the rhizosphere soil of Camellia oleifera plants, and the transparent circle method was used for rescreening. Only 4 strains of bacteria could form transparent circle on organophosphorus medium. The D/d value of 4 strains of bacteria was between 1.62 and 2.71, among which the D/d value of strain Y6 was the highest(2.71). The available phosphorus content of the fermentation supernatant was 8.50~14.79 mg/L, which was 7.88~14.17 mg/L higher than that of CK. The strain Y6 had the highest soluble phosphorus content of 14.79 mg/L in the fermentation supernatant, which was 14.17 mg/L higher than that of CK. According to the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis of strain Y6, it is preliminarily determined that strain Y6 is Pseudomonas. Strain Y6 is beneficial to improve the supply of phosphorus in rhizosphere soil of Camellia oleifera and promote the growth of Camellia oleifera. It has great potential in the development of bio-organic fertilizer.
