利用籼粳交珍汕97/武育粳2号F1花培获得的190个双单倍体群体(doubled-haploid population,DH系)及其构建的179个SSR分子标记遗传图谱,通过对DH系群体苗期重复接虫试验和2个不同时期对褐飞虱危害程度进行动态调查,并应用Mapmaker/exp Ver...利用籼粳交珍汕97/武育粳2号F1花培获得的190个双单倍体群体(doubled-haploid population,DH系)及其构建的179个SSR分子标记遗传图谱,通过对DH系群体苗期重复接虫试验和2个不同时期对褐飞虱危害程度进行动态调查,并应用Mapmaker/exp Version 3.0和Windows QTL Cartographer V 2.0对水稻抗褐飞虱数量性状基因位点(quantativetraitlocus,QTL)进行动态检测和遗传效应分析.结果表明,在苗期对褐飞虱抗性的检测中,共检测到6个抗性QTL,分别位于第2、3、4、8和10染色体上,各QTL的LOD值分别为2.22～4.64,贡献率为5.04%～13.73%,第3染色体和第4染色体上各有1个QTL的加性效应为正值,表明来自于亲本武育粳2号的这2个位点的等位基因可以提高水稻对褐飞虱的抗性,其余4个QTL的加性效应均为负值,表明来自于亲本珍汕97的这些位点的等位基因可以提高水稻对褐飞虱的抗性.展开更多
A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown p...A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown planthopper ( BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two-dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 ( pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of lice plants to BPH infestation.展开更多
2009年底，中国科学家在水稻稻飞虱抗性研究方面取得了令人瞩目的突破。由武汉大学何光存教授领导的课题组和国内外多位科学家合作，经过十多年不懈持续的研究，先后在《PNAS》和《Proteomics》发表文章，宣布克隆了水稻抗褐飞虱基因Bp...2009年底，中国科学家在水稻稻飞虱抗性研究方面取得了令人瞩目的突破。由武汉大学何光存教授领导的课题组和国内外多位科学家合作，经过十多年不懈持续的研究，先后在《PNAS》和《Proteomics》发表文章，宣布克隆了水稻抗褐飞虱基因Bph14，初步阐述了其介导的抗性机理，并从蛋白质组学水平对稻飞虱抗性机理进行分析研究（Duetal．，2009；Wei et al．，2009）。这无疑是中国科学家在水稻抗虫研究领域的重大贡献。展开更多
文摘利用籼粳交珍汕97/武育粳2号F1花培获得的190个双单倍体群体(doubled-haploid population,DH系)及其构建的179个SSR分子标记遗传图谱,通过对DH系群体苗期重复接虫试验和2个不同时期对褐飞虱危害程度进行动态调查,并应用Mapmaker/exp Version 3.0和Windows QTL Cartographer V 2.0对水稻抗褐飞虱数量性状基因位点(quantativetraitlocus,QTL)进行动态检测和遗传效应分析.结果表明,在苗期对褐飞虱抗性的检测中,共检测到6个抗性QTL,分别位于第2、3、4、8和10染色体上,各QTL的LOD值分别为2.22～4.64,贡献率为5.04%～13.73%,第3染色体和第4染色体上各有1个QTL的加性效应为正值,表明来自于亲本武育粳2号的这2个位点的等位基因可以提高水稻对褐飞虱的抗性,其余4个QTL的加性效应均为负值,表明来自于亲本珍汕97的这些位点的等位基因可以提高水稻对褐飞虱的抗性.
文摘A recombinant inbred population (RI) was constructed from a cross between B5, an introgression. line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 ( O. sativa L.). The brown planthopper ( BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two-dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 ( pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of lice plants to BPH infestation.
文摘2009年底，中国科学家在水稻稻飞虱抗性研究方面取得了令人瞩目的突破。由武汉大学何光存教授领导的课题组和国内外多位科学家合作，经过十多年不懈持续的研究，先后在《PNAS》和《Proteomics》发表文章，宣布克隆了水稻抗褐飞虱基因Bph14，初步阐述了其介导的抗性机理，并从蛋白质组学水平对稻飞虱抗性机理进行分析研究（Duetal．，2009；Wei et al．，2009）。这无疑是中国科学家在水稻抗虫研究领域的重大贡献。
