Several methods were tested for measurement of lysing cycle and burst size of the cyanophage infecting the filamentous cyanobacterium \%Plectonema boryanum\% IU594. The results indicated that the lysing curve of host ...Several methods were tested for measurement of lysing cycle and burst size of the cyanophage infecting the filamentous cyanobacterium \%Plectonema boryanum\% IU594. The results indicated that the lysing curve of host cells was consistent with the one step growth curve of cyanophage, thus it was more convenient to substitute measuring the lysing curve of host cells for the one step growth curve measurement of cyanophage. Besides,the burst size increased with the reducing cyanophage inoculation, reaching the maxium of 206PFU/Cell by 1PFU infection. It was suggested that more exact burst size of cyanophage could be obtained under 1PFU cyanophage inoculation.展开更多
Several studies have investigated the possible involvement of viral agents,and among them herpes viruses,in the development of cutaneous T- cell lymphoma. The aim of our study was to determine whether T- cells specifi...Several studies have investigated the possible involvement of viral agents,and among them herpes viruses,in the development of cutaneous T- cell lymphoma. The aim of our study was to determine whether T- cells specific to Epstein- Barr virus(EBV)- antigens were detectable among tumor- infiltrating lymphocytes infiltrating cutaneous lesions of a patient with Sé zary syndrome. To analyze responses to EBV, we used a transient SV- 40 origin- defective transformed simian cells transfection assay that permits an estimation of CD8 T- cell responses against a large number of HLA/viral protein combinations. This technique allowed the detection of EBV- specific T lymphocytes mainly directed against epitopes generated during the lytic cycle in the cutaneous lesions. This is, to our knowledge,the first descrip- tion of the presence of EBV- specific T lymphocytes among tumor- infiltrating lymphocytes infiltrating the lesional skin of a patient with Sé zary syndrome.展开更多
文摘Several methods were tested for measurement of lysing cycle and burst size of the cyanophage infecting the filamentous cyanobacterium \%Plectonema boryanum\% IU594. The results indicated that the lysing curve of host cells was consistent with the one step growth curve of cyanophage, thus it was more convenient to substitute measuring the lysing curve of host cells for the one step growth curve measurement of cyanophage. Besides,the burst size increased with the reducing cyanophage inoculation, reaching the maxium of 206PFU/Cell by 1PFU infection. It was suggested that more exact burst size of cyanophage could be obtained under 1PFU cyanophage inoculation.
文摘Several studies have investigated the possible involvement of viral agents,and among them herpes viruses,in the development of cutaneous T- cell lymphoma. The aim of our study was to determine whether T- cells specific to Epstein- Barr virus(EBV)- antigens were detectable among tumor- infiltrating lymphocytes infiltrating cutaneous lesions of a patient with Sé zary syndrome. To analyze responses to EBV, we used a transient SV- 40 origin- defective transformed simian cells transfection assay that permits an estimation of CD8 T- cell responses against a large number of HLA/viral protein combinations. This technique allowed the detection of EBV- specific T lymphocytes mainly directed against epitopes generated during the lytic cycle in the cutaneous lesions. This is, to our knowledge,the first descrip- tion of the presence of EBV- specific T lymphocytes among tumor- infiltrating lymphocytes infiltrating the lesional skin of a patient with Sé zary syndrome.
